Product Info Summary
SKU: | M03915 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-MYO7A Monoclonal Antibody
View all Myosin VIIa Antibodies
SKU/Catalog Number
M03915
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MYO7A Monoclonal Antibody catalog # M03915. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MYO7A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03915)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFCH-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Myosin VIIa Myosins
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M03915 is reactive to MYO7A in Human, Mouse, Rat
Applications
M03915 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
13 kDa
Calculated molecular weight
254.39kDa
Background of Myosin VIIa
Plays a major role in tight junction-specific obliteration of the intercellular space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Myosin VIIa expression in Y79 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For MYO7A (Source: Uniprot.org, NCBI)
Gene Name
MYO7A
Full Name
Unconventional myosin-VIIa
Weight
254.39kDa
Superfamily
TRAFAC class myosin-kinesin ATPase superfamily
Alternative Names
deafness, autosomal dominant 11; deafness, autosomal recessive 2; DFNA11; DFNB2; myosin VIIA; myosin-VIIa; MYOVIIA; MYU7A; USH1Bsevere)) MYO7A DFNA11, DFNB2, MYOVIIA, MYU7A, NSRD2, USH1B myosin VIIA unconventional myosin-VIIa|myosin VIIA (Usher syndrome 1B (autosomal recessive, severe))
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MYO7A, check out the MYO7A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MYO7A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MYO7A Monoclonal Antibody (M03915)
Hello CJ!
No publications found for M03915
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
6 Customer Q&As for Anti-MYO7A Monoclonal Antibody
Question
Is this M03915 anti-MYO7A Monoclonal antibody reactive to the isotypes of MYO7A?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
The immunogen of M03915 anti-MYO7A Monoclonal antibody is A synthesized peptide derived from human Myosin VIIa Myosins. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-05-04
Question
Is a blocking peptide available for product anti-MYO7A Monoclonal antibody (M03915)?
N. Anderson
Verified customer
Asked: 2019-12-04
Answer
We do provide the blocking peptide for product anti-MYO7A Monoclonal antibody (M03915). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-04
Question
I have attached the WB image, lot number and protocol we used for retina using anti-MYO7A Monoclonal antibody M03915. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-21
Question
We are currently using anti-MYO7A Monoclonal antibody M03915 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
The anti-MYO7A Monoclonal antibody (M03915) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-02
Question
I was wanting to use your anti-MYO7A Monoclonal antibody for WB for human retina on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human retina identification?
Verified Customer
Verified customer
Asked: 2018-01-08
Answer
As indicated on the product datasheet, M03915 anti-MYO7A Monoclonal antibody has been tested for Flow Cytometry, IF, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human retina in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-08
Question
Do you have a BSA free version of anti-MYO7A Monoclonal antibody M03915 available?
T. Kulkarni
Verified customer
Asked: 2014-11-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-MYO7A Monoclonal antibody M03915 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-11-10