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SKU:MA1067
Clonality:Monoclonal
Clone Number:GC-4
Application:IHC-F, WB
Price: $200.00
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Overview

Product Name Anti-Cadherin-2/N-Cadherin Antibody
SKU/Catalog Number MA1067
Description Mouse IgG monoclonal antibody for N-Cadherin, cadherin 2, type 1, N-cadherin (neuronal) (CDH2 ) detection. Tested with WB, IHC-F in Human;mouse;rat;rabbit;chicken. No cross reactivity with other proteins.
Cite This Product Anti-Cadherin-2/N-Cadherin Antibody GC-4 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1067)
Replacement Item This antibody may replace the following items: sc-11743|sc-366034|sc-11746|sc-390336|sc-390147 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Chicken, Human, Mouse, Rabbit, Rat
Predicted Species Monkey

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Affinity purified chicken heart A-CAM.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number GC-4
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Cdh2
Protein Name Cadherin-2
Molecular Weight 99686 MW
Protein Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity). .
Tissue Specificity In testis, expressed in Sertoli and germ cells. .
Subcellular Localization Cell membrane ; Single-pass type I membrane protein .
Uniprot ID Q9Z1Y3
Alternative Names Cadherin-2;Neural cadherin;N-cadherin;CD325;Cdh2;
Research Areas |signal transduction|cytoskeleton / ecm|cell adhesion|cadherins|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Cadherin-2

N-cadherin(NCAD) is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule(L-CAM). N-Cadherin,, also known as Cadherin-2, encodes a 907-amino acid protein that includes a 159-amino acid signal sequence. Human and mouse nucleotide sequences are 96% identical. Mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. Human N-cadherin gene contains 16 exons and its sequence is highly similar to both the mouse NCAD gene(including the large first and second introns) and other cadherin genes. N-cadherin is mapped to 18q11.2. Cadherin regulates dendritic spine morphogenesis.

Anti-Cadherin-2/N-Cadherin Antibody Images

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Anti-Cadherin-2/N-Cadherin Antibody
Anti-N-Cadherin antibody (monoclonal), MA1067, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Medulla Oblongata Tissue Lysate
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Publications

Yang N, Hui L, Wang Y, Yang H, Jiang X. Tumour Biol. 2014 Aug;35(8):7965-73. Doi: 10.1007/S13277-014-2045-3. Epub 2014 May 16. Overexpression Of Sox2 Promotes Migration, Invasion, And Epithelial-Mesenchymal Transition Through The Wnt/??-Catenin Pat...
Tong W, Sun D, Wang Q, Suo J. Cell Biochem Biophys. 2015 Jan 8. [Epub Ahead Of Print] Sorcin Enhances Metastasis And Promotes Epithelial-To-Mesenchymal Transition Of Colorectal Cancer.
Liu H, Liu Z, Jiang B, Peng R, Ma Z, Lu J. Cell Biochem Biophys. 2015 Feb 26. [Epub Ahead Of Print] Sox9 Overexpression Promotes Glioma Metastasis Via Wnt/??-Catenin Signaling.
Kudo-Sakamoto Y, Akazawa H, Ito K, Takano J, Yano M, Yabumoto C, Naito At, Oka T, Lee Jk, Sakata Y, Suzuki J, Saido Tc, Komuro I. J Biol Chem. 2014 Jul 11;289(28):19408-19. Doi: 10.1074/Jbc.M114.567206. Epub 2014 Jun 2. Calpain-Dependent Cleavage ...
Qiao B, Johnson Nw, Gao J. Int J Oncol. 2010 Sep;37(3):663-8. Epithelial-Mesenchymal Transition In Oral Squamous Cell Carcinoma Triggered By Transforming Growth Factor-Beta1 Is Snail Family-Dependent And Correlates With Matrix Metalloproteinase-2 ...

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.