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SKU:PA1328
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-Cadherin-2/N-Cadherin Antibody
SKU/Catalog Number PA1328
Description Rabbit IgG polyclonal antibody for Cadherin-2(CDH2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-Cadherin-2/N-Cadherin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1328)
Replacement Item This antibody may replace the following items: sc-11743|sc-366034|sc-11746|sc-390336|sc-390147 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Bovine

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human N Cadherin(701-714aa CQCDSNGDCTDVDR), identical to the related rat and mouse sequences.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name CDH2
Protein Name Cadherin-2
Molecular Weight 99809 MW
Protein Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity). .
Subcellular Localization Cell membrane; Single-pass type I membrane protein.
Uniprot ID P19022
Alternative Names Cadherin-2;CDw325;Neural cadherin;N-cadherin;CD325;CDH2;CDHN, NCAD;
Research Areas |signal transduction|cytoskeleton / ecm|cell adhesion|cadherins|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Cadherin-2

N-cadherin(NCAD) is a member of the cadherin cell-cell adhesion receptor family that includes P-, E-, and R-cadherin and liver cell adhesion molecule(L-CAM). N-Cadherin,, also known as Cadherin-2, encodes a 907-amino acid protein that includes a 159-amino acid signal sequence. Human and mouse nucleotide sequences are 96% identical. Mouse Ncad gene consists of 16 exons dispersed over more than 200 kb of genomic DNA. Human N-cadherin gene contains 16 exons and its sequence is highly similar to both the mouse NCAD gene(including the large first and second introns) and other cadherin genes. N-cadherin is mapped to 18q11.2. Cadherin regulates dendritic spine morphogenesis.

Anti-Cadherin-2/N-Cadherin Antibody Images

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Anti-Cadherin-2/N-Cadherin Antibody
Anti-N Cadherin antibody, PA1328, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Anti-Cadherin-2/N-Cadherin Antibody
Anti-N Cadherin antibody, PA1328, IHC(P)
IHC(P): Human Ovary Cancer Tissue
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Publications

Yang N, Hui L, Wang Y, Yang H, Jiang X. Tumour Biol. 2014 Aug;35(8):7965-73. Doi: 10.1007/S13277-014-2045-3. Epub 2014 May 16. Overexpression Of Sox2 Promotes Migration, Invasion, And Epithelial-Mesenchymal Transition Through The Wnt/??-Catenin Pat...
Tong W, Sun D, Wang Q, Suo J. Cell Biochem Biophys. 2015 Jan 8. [Epub Ahead Of Print] Sorcin Enhances Metastasis And Promotes Epithelial-To-Mesenchymal Transition Of Colorectal Cancer.
Liu H, Liu Z, Jiang B, Peng R, Ma Z, Lu J. Cell Biochem Biophys. 2015 Feb 26. [Epub Ahead Of Print] Sox9 Overexpression Promotes Glioma Metastasis Via Wnt/??-Catenin Signaling.
Kudo-Sakamoto Y, Akazawa H, Ito K, Takano J, Yano M, Yabumoto C, Naito At, Oka T, Lee Jk, Sakata Y, Suzuki J, Saido Tc, Komuro I. J Biol Chem. 2014 Jul 11;289(28):19408-19. Doi: 10.1074/Jbc.M114.567206. Epub 2014 Jun 2. Calpain-Dependent Cleavage ...
Qiao B, Johnson Nw, Gao J. Int J Oncol. 2010 Sep;37(3):663-8. Epithelial-Mesenchymal Transition In Oral Squamous Cell Carcinoma Triggered By Transforming Growth Factor-Beta1 Is Snail Family-Dependent And Correlates With Matrix Metalloproteinase-2 ...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is n-cadherin antibody