Data & Images
|Product Name||Anti-CD56/NCAM-1 Antibody|
|Description||Mouse IgG monoclonal antibody for NCAM, neural cell adhesion molecule 1 (NCAM1) detection. Tested with WB, ICC in Human;mouse;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-CD56/NCAM-1 Antibody NCAM-0B11 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1068)|
|Replacement Item||This antibody may replace the following items: sc-106|sc-10735|sc-1507|sc-31035|sc-374032|sc-374289|sc-51742|sc-53007|sc-59862|sc-59864|sc-65547|sc-71647|sc-7326|sc-73555 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Growth cone enriched plasma membrane fraction from E17 rat forebrain.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Neural cell adhesion molecule 1|
|Molecular Weight||94658 MW|
|Protein Function||This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.|
|Sequence Similarities||Contains 2 fibronectin type-III domains.|
|Subcellular Localization||Cell membrane; Single-pass type I membrane protein.|
|Alternative Names||Neural cell adhesion molecule 1;N-CAM-1;NCAM-1;CD56;Ncam1;Ncam;|
|Research Areas|||neuroscience|cell adhesion proteins|membrane proteins| neuroscience|neurology process|growth and development|axonal guidance proteins| developmental biology|organogenesis|nervous system development| kits/ lysates/ other|elisa kits|cd markers elisa kits|neuroscience target elisa kits||
Background for Neural cell adhesion molecule 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CD56/NCAM-1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -|
Western blot, 4μg/ml, Human, mouse, rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CD56/NCAM-1 Antibody Images
Click the images to enlarge.
ICC: A549 Cell
ICC: NEURO-2?? Cell
ICC: SMMC-7721 Cell