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Product Info Summary
| SKU: | A00729 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-NCAM-L1 L1CAM Antibody
SKU/Catalog Number
A00729
Size
100μl
Form
Liquid
Description
Boster Bio Anti-NCAM-L1 L1CAM Antibody catalog # A00729. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NCAM-L1 L1CAM Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00729)
Host
Rabbit
Contents
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Isotype
IgG
Immunogen
Synthetic peptide from human protein at AA range: 151-200.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00729 is reactive to L1CAM in Human, Mouse, Rat
Applications
A00729 is guaranteed for IHC Boster Guarantee
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
IHC-P, 1:50-300
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human-stomach-cancer, antibody was diluted at 1:200.
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Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.
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Immunohistochemical analysis of paraffin-embedded human-stomach-cancer, antibody was diluted at 1:200.
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Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200.
Protein Target Info & Infographic
Gene/Protein Information For L1CAM (Source: Uniprot.Org, NCBI)
Uniprot ID
P32004
Gene ID
3897
Gene Name
L1CAM
Full Name
Neural cell adhesion molecule L1
Weight
140.003kDa
Superfamily
immunoglobulin superfamily
Alternative Names
antigen identified by monoclonal R1; CAML1; CAML1N-CAML1; CD171 antigen; CD171; HSAS; HSAS1; L1 cell adhesion molecule; L1CAM; MASA; MIC5; N-CAM-L1; NCAM-L1; neural cell adhesion molecule L1; S10; SPG1 L1CAM CAML1, CD171, HSAS, HSAS1, MASA, MIC5, N-CAM-L1, N-CAML1, NCAM-L1, S10, SPG1 L1 cell adhesion molecule neural cell adhesion molecule L1|antigen identified by monoclonal antibody R1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on L1CAM, check out the L1CAM Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for L1CAM: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Anti-NCAM-L1 L1CAM Antibody (A00729)
No publications found for A00729
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Recommended Resources
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- Boster's Pathways Library
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-NCAM-L1 L1CAM Antibody
Question
Our lab were satisfied with the WB result of your anti-NCAM-L1 antibody. However we have seen positive staining in right hemisphere of cerebellum cell membrane using this antibody. Is that expected? Could you tell me where is L1CAM supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
From literature, right hemisphere of cerebellum does express L1CAM. Generally L1CAM expresses in cell membrane. Regarding which tissues have L1CAM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231, 23186163
Fetal brain, Pubmed ID: 1769655, 1923824, 1932117
Liver, Pubmed ID: 19159218
Pancreas, Pubmed ID: 15489334
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2020-04-20
Question
I was wanting to use your anti-NCAM-L1 antibody for IHC for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
As indicated on the product datasheet, A00729 anti-NCAM-L1 antibody has been tested for IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-09
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-NCAM-L1 antibody A00729. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-02
Question
Is a blocking peptide available for product anti-NCAM-L1 antibody (A00729)?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
We do provide the blocking peptide for product anti-NCAM-L1 antibody (A00729). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-10
Question
I have attached the WB image, lot number and protocol we used for cervix carcinoma using anti-NCAM-L1 antibody A00729. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-27
Question
you antibody to test anti-NCAM-L1 antibody A00729 on human cervix carcinoma for research purposes, then I may be interested in using anti-NCAM-L1 antibody A00729 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
The products we sell, including anti-NCAM-L1 antibody A00729, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-21
Question
We have been able to see staining in mouse cervix carcinoma. Do you have any suggestions? Is anti-NCAM-L1 antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
From literature cervix carcinoma does express L1CAM. From Uniprot.org, L1CAM is expressed in right hemisphere of cerebellum, fetal brain, pancreas, plasma, cervix carcinoma, liver, among other tissues. Regarding which tissues have L1CAM expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231, 23186163
Fetal brain, Pubmed ID: 1769655, 1923824, 1932117
Liver, Pubmed ID: 19159218
Pancreas, Pubmed ID: 15489334
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2019-08-12
Question
Do you have a BSA free version of anti-NCAM-L1 antibody A00729 available?
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-NCAM-L1 antibody A00729 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-02
Question
We are currently using anti-NCAM-L1 antibody A00729 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-04
Answer
The anti-NCAM-L1 antibody (A00729) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-04
Question
Does A00729 anti-NCAM-L1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-01-21
Answer
As indicated on the product datasheet, A00729 anti-NCAM-L1 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-21
Question
Is this A00729 anti-NCAM-L1 antibody reactive to the isotypes of L1CAM?
A. Yang
Verified customer
Asked: 2019-01-08
Answer
The immunogen of A00729 anti-NCAM-L1 antibody is Synthetic peptide from human protein at AA range: 151-200. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-08
Question
I see that the anti-NCAM-L1 antibody A00729 works with IHC, what is the protocol used to produce the result images on the product page?
E. Bhatt
Verified customer
Asked: 2017-03-29
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-03-29
Question
Will anti-NCAM-L1 antibody A00729 work for IHC with cervix carcinoma?
A. Jackson
Verified customer
Asked: 2013-08-29
Answer
According to the expression profile of cervix carcinoma, L1CAM is highly expressed in cervix carcinoma. So, it is likely that anti-NCAM-L1 antibody A00729 will work for IHC with cervix carcinoma.
Boster Scientific Support
Answered: 2013-08-29
Question
My question regarding product A00729, anti-NCAM-L1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Jones
Verified customer
Asked: 2013-04-16
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00729 anti-NCAM-L1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-04-16
