Anti-Nicastrin/NCSTN Antibody

Nicastrin antibody

Boster Bio Anti-Nicastrin/NCSTN Antibody catalog # PA2250. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PA2250
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-Nicastrin/NCSTN Antibody

View all Nicastrin Antibodies

SKU/Catalog Number

PA2250

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Nicastrin/NCSTN Antibody catalog # PA2250. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Nicastrin/NCSTN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2250)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Nicastrin, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2250 is reactive to NCSTN in Human

Applications

PA2250 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

120-130 kDa

Calculated molecular weight

78.411kDa

Background of Nicastrin

Nicastrin, also known as NCSTN, is a protein that is a part of the gamma secretase protein complex. It is mapped to 1q23.2. This gene is not catalytically active, but instead promotes the maturation and proper trafficking of the other proteins in the gamma secretase protein complex, all of which undergo significant post-translational modification before becoming active in the cell. NCSTN encodes a Type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease. Nicastrin has also been identified as a regulator of neprilysin, an enzyme involved in the degradation of amyloid beta fragment.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For NCSTN (Source: Uniprot.org, NCBI)

Gene Name

NCSTN

Full Name

Nicastrin

Weight

78.411kDa

Superfamily

nicastrin family

Alternative Names

anterior pharynx-defective 2; APH2; ATAG1874; KIAA0253APH2; NCSTN; Nicastrin NCSTN ATAG1874 nicastrin nicastrin|anterior pharynx-defective 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NCSTN, check out the NCSTN Infographic

NCSTN infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NCSTN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-Nicastrin/NCSTN Antibody

Question

We are currently using anti-Nicastrin/NCSTN antibody PA2250 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?

J. Zhao

Verified customer

Asked: 2016-06-07

Answer

The anti-Nicastrin/NCSTN antibody (PA2250) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-06-07

Order DetailsPrice
PA2250

100μg

$370
PA2250-10ug

10μg sample (liquid)

$99
PA2250-Biotin

100 μg Biotin conjugated

$570
PA2250-Cy3

100 μg Cy3 conjugated

$570
PA2250-Dylight488

100 μg Dylight488 conjugated

$570
PA2250-Dylight550

100 μg Dylight550 conjugated

$570
PA2250-Dylight594

100 μg Dylight594 conjugated

$570
PA2250-FITC

100 μg FITC conjugated

$570
PA2250-HRP

100 μg HRP conjugated

$570
PA2250-APC

100 μg APC conjugated

$670
PA2250-PE

100 μg PE conjugated

$670
PA2250-carrier-free

Carrier Free

$370

More conjugates/formats

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PA2250
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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