|Application:||IHC-P, IHC-F, ICC, WB|
Data & Images
|Product Name||Anti-NDUFA1 Antibody|
|Description||Rabbit IgG polyclonal antibody for NADH dehydrogenase[ubiquinone] 1 alpha subcomplex subunit 1(NDUFA1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-NDUFA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1661)|
|Replacement Item||This antibody may replace the following items: sc-131623|sc-131625 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
|Application||IHC-P, IHC-F, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human NDUFA1(54-70aa ISGVDRYYVSKGLENID), different from the related rat and mouse sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||NADH dehydrogenase[ubiquinone] 1 alpha subcomplex subunit 1|
|Molecular Weight||8072 MW|
|Protein Function||Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.|
|Tissue Specificity||Primarily expressed in heart and skeletal muscle.|
|Subcellular Localization||Mitochondrion inner membrane; Single-pass membrane protein; Matrix side.|
|Alternative Names||NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1;Complex I-MWFE;CI-MWFE;NADH-ubiquinone oxidoreductase MWFE subunit;NDUFA1;|
|Research Areas|||tags & cell markers|subcellular markers|organelles|mitochondria| signal transduction|metabolism|mitochondrial| cancer|cancer metabolism|metabolic signaling pathway|integration of energy metabolism| metabolism|pathways and processes|mitochondrial metabolism|mitochondrial markers|metabolic signaling pathways|energy transfer pathways|integration of energy|oxidative phosphorylation|complex i||
Background for NADH dehydrogenase[ubiquinone] 1 alpha subcomplex subunit 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-NDUFA1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-NDUFA1 Antibody Images
Click the images to enlarge.
Lane 1: Rat Cardiac Muscle Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: Rat Kidney Tissue Lysate
Lane 4: Rat Brain Tissue Lysate
Lane 5: HT1080 Cell Lysate
IHC(P): Rat Cardiac Muscle Tissue
IHC(F): Rat Cardiac Muscle Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,