Anti-NEB2 PPP1R9B Antibody

PPP1R9B/Spinophilin antibody

Boster Bio Anti-NEB2 PPP1R9B Antibody catalog # A07437. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A07437
Size: 100ul
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, WB

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Product Name

Anti-NEB2 PPP1R9B Antibody

View all PPP1R9B/Spinophilin Antibodies

SKU/Catalog Number

A07437

Size

100ul

Form

Liquid

Description

Boster Bio Anti-NEB2 PPP1R9B Antibody catalog # A07437. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NEB2 PPP1R9B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07437)

Host

Rabbit

Contents

Liquid form in PBS containing 50% glycerol,and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human NEB2 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A07437 is reactive to Ppp1r9b in Human, Mouse, Rat

Applications

A07437 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

89.646kDa

Background of PPP1R9B/Spinophilin

Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5'-end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure.

Izaurralde E., Cell 78:657-668(1994).
Kataoka N., Nucleic Acids Res. 22:3861-3865(1994).
Humphray S.J., Nature 429:369-374(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-2000 ELISA 1:5000-20000

Validation Images & Assay Conditions

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Gene/Protein Information For Ppp1r9b (Source: Uniprot.org, NCBI)

Gene Name

Ppp1r9b

Full Name

Neurabin-2

Weight

89.646kDa

Alternative Names

FLJ30345; neurabin II; Neurabin-2; neurabin-II; PPP1R6; PPP1R9; PPP1R9B; Protein phosphatase 1 regulatory subunit 9B; protein phosphatase 1, regulatory (inhibitor) subunit 9B; protein phosphatase 1, regulatory subunit 9B, spinophilin; SPINO; Spinophilin; Spn Ppp1r9b|Neb2|protein phosphatase 1, regulatory subunit 9B|neurabin-2|PP1bp134|neurabin-II|neural tissue-specific F-actin-binding protein II|nuerabin 2|p130|spinophilin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Ppp1r9b, check out the Ppp1r9b Infographic

Ppp1r9b infographic

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In this infographic, you will see the following information for Ppp1r9b: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A07437-100ul

100ul

$380

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