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SKU:MA1069
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:NB2
Application:WB
Price: $200.00
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Overview

Product Name Anti-Nebulin Antibody (Monoclonal, NB2)
SKU/Catalog Number MA1069
Description Mouse IgG monoclonal antibody for Nebulin, nebulin (NEB) detection. Tested with WB in chicken. No cross reactivity with other proteins.
Cite This Product Anti-Nebulin Antibody (Monoclonal, NB2) NB2 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1069)
Replacement Item This antibody may replace the following items: sc-28286|sc-8082|sc-8083 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Chicken
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Chicken breast nebulin.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number NB2
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name NEB
Protein Name Nebulin
Molecular Weight 772914 MW
Protein Function This giant muscle protein may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. Binds and stabilize F-actin.
Tissue Specificity Muscle specific. Located in the thin filament of striated muscle.
Sequence Similarities Contains 178 nebulin repeats.
Subcellular Localization Cytoplasm, myofibril, sarcomere. Cytoplasm, cytoskeleton.
Uniprot ID P20929
Alternative Names Nebulin;NEB;
Research Areas |signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|actin etc|actin binding proteins|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Nebulin

Nebulin is a giant protein component of the cytoskeletal matrix that coexists with the thick and thin filaments within the sarcomeres of skeletal muscle. In most vertebrates, nebulin accounts for 3 to 4% of the total myofibrillar protein and its size varies from 600 to 800 kD in a manner that is tissue-, species-, and developmental stage-specific. The nebulin gene contains 183 exons in a 249-kb genomic region. Nebulin is mapped to chromosome 2. Nebulin is a giant filamentous protein specific for vertebrate skeletal muscles. Mutations in the nebulin gene associated with autosomal recessive nemaline myopathy.

Anti-Nebulin Antibody (Monoclonal, NB2) Images

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Anti-Nebulin Antibody (Monoclonal, NB2)
Anti- Nebulin antibody, MA1069, Western blotting
All lanes: Anti Nebulin (MA1069) at 0.5ug/ml
WB: Mouse Skeletal Muscle Tissue Lysate at 50ug
Predicted bind size: 773KD
Observed bind size: 773KD
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Publications

Li X, Ni M, Zhang C, Ma W, Zhang Y. Rna. 2014 Feb;20(2):252-9. Doi: 10.1261/Rna.040220.113. Epub 2013 Dec 17. A Convenient System For Highly Specific And Sensitive Detection Of Mirna Expression.

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.