Data & Images
|Product Name||Anti-Nebulin Antibody (Monoclonal, NB2)|
|Description||Mouse IgG monoclonal antibody for Nebulin, nebulin (NEB) detection. Tested with WB in chicken. No cross reactivity with other proteins.|
|Cite This Product||Anti-Nebulin Antibody (Monoclonal, NB2) NB2 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1069)|
|Replacement Item||This antibody may replace the following items: sc-28286|sc-8082|sc-8083 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Chicken breast nebulin.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||772914 MW|
|Protein Function||This giant muscle protein may be involved in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. Binds and stabilize F-actin.|
|Tissue Specificity||Muscle specific. Located in the thin filament of striated muscle.|
|Sequence Similarities||Contains 178 nebulin repeats.|
|Subcellular Localization||Cytoplasm, myofibril, sarcomere. Cytoplasm, cytoskeleton.|
|Research Areas|||signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|actin etc|actin binding proteins||
Background for Nebulin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Nebulin Antibody (Monoclonal, NB2) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 1-2 μg/ml, Chicken|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Nebulin Antibody (Monoclonal, NB2) Images
Click the images to enlarge.
All lanes: Anti Nebulin (MA1069) at 0.5ug/ml
WB: Mouse Skeletal Muscle Tissue Lysate at 50ug
Predicted bind size: 773KD
Observed bind size: 773KD