|Application:||IHC-P, IHC-F, WB|
Data & Images
|Product Name||Anti-NEFH Antibody (Monoclonal, N52)|
|Description||Mouse IgG monoclonal antibody for NEFH, neurofilament, heavy polypeptide (NEFH) detection. Tested with WB, IHC-P, IHC-F in Human;mouse;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-NEFH Antibody (Monoclonal, N52) N52 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1071)|
|Replacement Item||This antibody may replace the following items: sc-133165|sc-133237|sc-137009|sc-20014|sc-20015|sc-20112|sc-22909|sc-32273|sc-32728|sc-32729|sc-32730|sc-51682|sc-52350|sc-53798|sc-56575|sc-58553 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
|Application||IHC-P, IHC-F, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||C-terminal segment of enzymatically dephosphorylated pig Neurofilament 200.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Neurofilament heavy polypeptide|
|Molecular Weight||115378 MW|
|Protein Function||Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.|
|Sequence Similarities||Belongs to the intermediate filament family.|
|Alternative Names||Neurofilament heavy polypeptide;NF-H;200 kDa neurofilament protein;Neurofilament triplet H protein;Nefh;Nfh;|
|Research Areas|||neuroscience|neurology process|neurodegenerative disease|alzheimer's disease| neuroscience|cell adhesion proteins|cytoskeletal proteins|intermediate filaments| signal transduction|cytoskeleton / ecm|cytoskeleton|class iv|neurofilaments|cell type marker|neuron marker|axon marker||
Background for Neurofilament heavy polypeptide
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-NEFH Antibody (Monoclonal, N52) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, mouse, rat, By Heat
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, mouse, rat, -
Western blot, 0.5ml, Human, mouse, rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-NEFH Antibody (Monoclonal, N52) Images
Click the images to enlarge.
IHC(P): Rat Brain Tissue