Product Info Summary
SKU: | M06821 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-NEFM/Nf M Rabbit Monoclonal Antibody
SKU/Catalog Number
M06821
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NEFM/Nf M Rabbit Monoclonal Antibody catalog # M06821. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NEFM/Nf M Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06821)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAGI-14
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NEFM
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M06821 is reactive to NEFM in Human
Applications
M06821 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
94 kDa
Calculated molecular weight
102.472kDa
Background of NF-M
Putative transcription factor involved in pancreas development and function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:20
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:3W dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of NEFM expression in 293T cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For NEFM (Source: Uniprot.org, NCBI)
Gene Name
NEFM
Full Name
Neurofilament medium polypeptide
Weight
102.472kDa
Superfamily
intermediate filament family
Alternative Names
NEF3; NEF3neurofilament medium polypeptide; NEFM; Neurofilament 3; neurofilament, medium polypeptide 150kDa; neurofilament, medium polypeptide; neurofilament-3 (150 kD medium); NFM; NF-M; NF-M160 kDa neurofilament protein; NFMNeurofilament triplet M protein NEFM NEF3, NF-M, NFM neurofilament medium neurofilament medium polypeptide|160 kDa neurofilament protein|neurofilament 3|neurofilament triplet M protein|neurofilament, medium polypeptide 150kDa|neurofilament-3 (150 kD medium)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NEFM, check out the NEFM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NEFM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NEFM/Nf M Rabbit Monoclonal Antibody (M06821)
Hello CJ!
No publications found for M06821
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-NEFM/Nf M Rabbit Monoclonal Antibody
Question
Do you have the sequence and uniprot number for M06821?
Verified customer
Asked: 2022-11-29
Answer
The sequence is CVEEKGK-pS-PVSKSPV (Uniprot#P07197).
Boster Scientific Support
Answered: 2022-11-29