Product Info Summary
SKU: | A01919 |
---|---|
Size: | 100μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Neuromedin-U NMU Antibody
View all Neuromedin-U Antibodies
SKU/Catalog Number
A01919
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Neuromedin-U NMU Antibody catalog # A01919. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Neuromedin-U NMU Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01919)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human NMU at amino acid range 125-174
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01919 is reactive to NMU in Human
Applications
A01919 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
19.741kDa
Background of Neuromedin-U
CaM Kinase IV (also known as CAM kinase-GR and CaMK IV) is a calcium/ calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. This kinase may be involved in the transcriptional regulation of microtubule dynamics. In vitro, CaMK IV phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. CaMK IV may also be involved in spermatogenesis and may play a role in the consolidation/ retention of hippocampus-dependent long-term memory. CaMK IV must be phosphorylated to be maximally active and is phosphorylated by CAMKK1 or CAMKK2. In addition autophosphorylation of the N-terminus is required for full activation. Autophosphorylation of Ser-336 allows the kinase to switch to a Ca(2+)/calmodulin-independent state. Most likely the kinase is inactivated by the serine/ threonine protein phosphatase 2A. CaMK IV is a monomer that is located within the cytoplasm and nucleus and substantial localization occurs in certain neuronal nuclei. In spermatids CaMK IV is associated with chromatin and the nuclear matrix. CaMK IV is also specifically expressed in epithelial ovarian cancer tissue.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of various cells using Neuromedin-U Polyclonal Antibody diluted at 1:500
Protein Target Info & Infographic
Gene/Protein Information For NMU (Source: Uniprot.org, NCBI)
Gene Name
NMU
Full Name
Neuromedin-U
Weight
19.741kDa
Superfamily
NmU family
Alternative Names
neuromedin U; neuromedin-U NMU neuromedin U neuromedin-U|neuromedin U precursor-related peptide/neuromedin U preproprotein|prepro-NMU
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NMU, check out the NMU Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NMU: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Neuromedin-U NMU Antibody (A01919)
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No publications found for A01919
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Neuromedin-U NMU Antibody
Question
I see that the anti-Neuromedin-U antibody A01919 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-12-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-12-19
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pituitary using anti-Neuromedin-U antibody A01919. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-13
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-13
Question
Is this A01919 anti-Neuromedin-U antibody reactive to the isotypes of NMU?
Verified Customer
Verified customer
Asked: 2017-11-29
Answer
The immunogen of A01919 anti-Neuromedin-U antibody is Synthesized peptide derived from the C-terminal region of human Neuromedin-U. at AA rangle: 120-200. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-29
Question
Does anti-Neuromedin-U antibody A01919 work for WB with pituitary?
Verified Customer
Verified customer
Asked: 2017-07-06
Answer
According to the expression profile of pituitary, NMU is highly expressed in pituitary. So, it is likely that anti-Neuromedin-U antibody A01919 will work for WB with pituitary.
Boster Scientific Support
Answered: 2017-07-06
Question
My lab would like to test anti-Neuromedin-U antibody A01919 on human pituitary for research purposes, then I may be interested in using anti-Neuromedin-U antibody A01919 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-06-19
Answer
The products we sell, including anti-Neuromedin-U antibody A01919, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-06-19
Question
Is there a BSA free version of anti-Neuromedin-U antibody A01919 available?
R. Parker
Verified customer
Asked: 2015-06-12
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Neuromedin-U antibody A01919 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-06-12
Question
Is a blocking peptide available for product anti-Neuromedin-U antibody (A01919)?
K. Huang
Verified customer
Asked: 2013-06-10
Answer
We do provide the blocking peptide for product anti-Neuromedin-U antibody (A01919). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-06-10