Anti-Neuropilin 1/NRP1 Antibody Picoband™

Neuropilin-1 antibody

Boster Bio Anti-Neuropilin 1/NRP1 Antibody Picoband™ catalog # PB9300. Tested in IHC, WB applications. This antibody reacts with Human, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: PB9300
Size: 100 μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Neuropilin 1/NRP1 Antibody Picoband™

View all Neuropilin-1 Antibodies

SKU/Catalog Number

PB9300

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Neuropilin 1/NRP1 Antibody Picoband™ catalog # PB9300. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Neuropilin 1/NRP1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9300)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Neuropilin 1 recombinant protein (Position: K504-T827). Human Neuropilin 1 shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Neuropilin 1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9300 is reactive to NRP1 in Human, Rat

Applications

PB9300 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

103 kDa

Calculated molecular weight

103.134kDa

Background of Neuropilin-1

This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contain a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors; they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat

Validation Images & Assay Conditions

Gene/Protein Information For NRP1 (Source: Uniprot.org, NCBI)

Gene Name

NRP1

Full Name

Neuropilin-1

Weight

103.134kDa

Superfamily

neuropilin family

Alternative Names

BDCA4; BDCA-4; CD304 antigen; CD304; DKFZp686A03134; DKFZp781F1414; neuropilin 1; Neuropilin1; Neuropilin-1; NRP1; NRPNP1; transmembrane receptor; Vascular endothelial cell growth factor 165 receptor; VEGF165RCD304 NRP1 BDCA4, CD304, NP1, NRP, VEGF165R neuropilin 1 neuropilin-1|transmembrane receptor|vascular endothelial cell growth factor 165 receptor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NRP1, check out the NRP1 Infographic

NRP1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NRP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9300 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Dual Receptor Recognizing Cell Penetrating Peptide for Selective Targeting, Efficient Intratumoral Diffusion and Synthesized Anti-Glioma Therapy

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16 Customer Q&As for Anti-Neuropilin 1/NRP1 Antibody Picoband™

Question

We are interested in using your anti-Neuropilin 1/NRP1 antibody for semaphorin-plexin signaling pathway involved in neuron projection guidance studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-03-25

Answer

We appreciate your inquiry. This PB9300 anti-Neuropilin 1/NRP1 antibody is tested on u87 whole cell lysate, a549 whole cell lysate, human placenta tissue, tissue lysate, rat cardiac muscle tissue lysate. It is guaranteed to work for IHC, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-03-25

Question

Do you have a BSA free version of anti-Neuropilin 1/NRP1 antibody PB9300 available?

Verified Customer

Verified customer

Asked: 2020-03-04

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Neuropilin 1/NRP1 antibody PB9300 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-03-04

Question

We ordered your anti-Neuropilin 1/NRP1 antibody for WB on prostatic adenocarcinoma a few months ago. I am using rat, and We want to use the antibody for IHC next. you antibody examining prostatic adenocarcinoma as well as caput epididymis in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

I took a look at the website and datasheets of our anti-Neuropilin 1/NRP1 antibody and it seems that PB9300 has been validated on rat in both WB and IHC. Thus PB9300 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-02-28

Question

Would PB9300 anti-Neuropilin 1/NRP1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-11-13

Answer

You can see on the product datasheet, PB9300 anti-Neuropilin 1/NRP1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-11-13

Question

I was wanting to use your anti-Neuropilin 1/NRP1 antibody for WB for human caput epididymis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human caput epididymis identification?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

You can see on the product datasheet, PB9300 anti-Neuropilin 1/NRP1 antibody has been validated for IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human caput epididymis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-08-12

Question

Our team were satisfied with the WB result of your anti-Neuropilin 1/NRP1 antibody. However we have observed positive staining in plasma cell membrane using this antibody. Is that expected? Could you tell me where is NRP1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

From literature, plasma does express NRP1. Generally NRP1 expresses in cell membrane. Regarding which tissues have NRP1 expression, here are a few articles citing expression in various tissues:
Kidney, and Muscle, Pubmed ID: 15489334
Liver, Pubmed ID: 19159218, 24275569
Mammary gland, Pubmed ID: 9529250
Plasma, Pubmed ID: 16335952
Prostatic adenocarcinoma, Pubmed ID: 10688880
Skeletal muscle, Pubmed ID: 17974005

Boster Scientific Support

Answered: 2019-08-09

Question

Would anti-Neuropilin 1/NRP1 antibody PB9300 work for WB with caput epididymis?

Verified Customer

Verified customer

Asked: 2019-08-08

Answer

According to the expression profile of caput epididymis, NRP1 is highly expressed in caput epididymis. So, it is likely that anti-Neuropilin 1/NRP1 antibody PB9300 will work for WB with caput epididymis.

Boster Scientific Support

Answered: 2019-08-08

Question

We have observed staining in human kidney muscle. Do you have any suggestions? Is anti-Neuropilin 1/NRP1 antibody supposed to stain kidney muscle positively?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

From what I have seen in literature kidney muscle does express NRP1. From what I have seen in Uniprot.org, NRP1 is expressed in caput epididymis, mammary gland, prostatic adenocarcinoma, skeletal muscle, kidney muscle, plasma, liver, among other tissues. Regarding which tissues have NRP1 expression, here are a few articles citing expression in various tissues:
Kidney, and Muscle, Pubmed ID: 15489334
Liver, Pubmed ID: 19159218, 24275569
Mammary gland, Pubmed ID: 9529250
Plasma, Pubmed ID: 16335952
Prostatic adenocarcinoma, Pubmed ID: 10688880
Skeletal muscle, Pubmed ID: 17974005

Boster Scientific Support

Answered: 2019-06-11

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for caput epididymis using anti-Neuropilin 1/NRP1 antibody PB9300. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-03-15

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-03-15

Question

My lab would like to test anti-Neuropilin 1/NRP1 antibody PB9300 on human caput epididymis for research purposes, then I may be interested in using anti-Neuropilin 1/NRP1 antibody PB9300 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-03-15

Answer

The products we sell, including anti-Neuropilin 1/NRP1 antibody PB9300, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-03-15

Question

Please see the WB image, lot number and protocol we used for caput epididymis using anti-Neuropilin 1/NRP1 antibody PB9300. Please let me know if you require anything else.

A. Miller

Verified customer

Asked: 2018-01-16

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-01-16

Question

Is a blocking peptide available for product anti-Neuropilin 1/NRP1 antibody (PB9300)?

K. Dhar

Verified customer

Asked: 2017-12-14

Answer

We do provide the blocking peptide for product anti-Neuropilin 1/NRP1 antibody (PB9300). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-12-14

Question

We are currently using anti-Neuropilin 1/NRP1 antibody PB9300 for rat tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on goat tissues as well?

P. Brown

Verified customer

Asked: 2017-11-27

Answer

The anti-Neuropilin 1/NRP1 antibody (PB9300) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-11-27

Question

I see that the anti-Neuropilin 1/NRP1 antibody PB9300 works with WB, what is the protocol used to produce the result images on the product page?

B. Rodriguez

Verified customer

Asked: 2017-08-24

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-08-24

Question

Can you help my question with product PB9300, anti-Neuropilin 1/NRP1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-07-26

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9300 anti-Neuropilin 1/NRP1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-07-26

Question

Is this PB9300 anti-Neuropilin 1/NRP1 antibody reactive to the isotypes of NRP1?

D. Jones

Verified customer

Asked: 2017-04-11

Answer

The immunogen of PB9300 anti-Neuropilin 1/NRP1 antibody is E.coli-derived human Neuropilin 1 recombinant protein (Position: K504-T827). Human Neuropilin 1 shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Neuropilin 1, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-04-11

Order DetailsPrice
PB9300

100μg

$370
PB9300-10ug

10μg sample (liquid)

$99
PB9300-Biotin

100 μg Biotin conjugated

$570
PB9300-Cy3

100 μg Cy3 conjugated

$570
PB9300-Dylight488

100 μg Dylight488 conjugated

$570
PB9300-Dylight550

100 μg Dylight550 conjugated

$570
PB9300-Dylight594

100 μg Dylight594 conjugated

$570
PB9300-FITC

100 μg FITC conjugated

$570
PB9300-HRP

100 μg HRP conjugated

$570
PB9300-APC

100 μg APC conjugated

$670
PB9300-PE

100 μg PE conjugated

$670
PB9300-carrier-free

Carrier Free

$370

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