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SKU:MA1070
Clonality:Monoclonal
Clone Number:NR4
Application:IHC-P, IHC-F, WB
Price: $200.00
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Overview

Product Name Anti-NF68 Antibody (Monoclonal, NR4)
Description Mouse IgG monoclonal antibody for NF68, neurofilament, light polypeptide (NEFL ) detection. Tested with WB, IHC-P, IHC-F in Human;pig;rat. No cross reactivity with other proteins.
Cite This Product Anti-NF68 Antibody (Monoclonal, NR4) NR4 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1070)
Replacement Item This antibody may replace the following items: sc-12965|sc-12965-R|sc-12966|sc-12980|sc-16667|sc-16667-R|sc-20012|sc-25652|sc-390732|sc-56575|sc-58559|sc-58560|sc-71678 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human, Pig, Rat
Application IHC-P, IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Pig spinal cord.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number NR4
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Nefl
Protein Name Neurofilament light polypeptide
Molecular Weight 61335 MW
Protein Function Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Sequence Similarities Belongs to the intermediate filament family.
Uniprot ID P19527
Alternative Names Neurofilament light polypeptide;NF-L;68 kDa neurofilament protein;Neurofilament triplet L protein;Nefl;Nf68, Nfl;
Research Areas |neuroscience|neurology process|neurodegenerative disease|alzheimer's disease| neuroscience|cell adhesion proteins|cytoskeletal proteins|intermediate filaments| signal transduction|cytoskeleton / ecm|cytoskeleton|class iv|neurofilaments| stem cells|lineage markers|ectoderm|cell type marker|neuron marker|axon marker| developmental biology|lineage specification|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Neurofilament light polypeptide

Neurofilaments are composed of 3 neuron-specific proteins with apparent molecular masses of 68 kD(NFL), 125 kD(NFM) and 200 kD(NFH) on SDS-gel electrophoresis. And they have a role in the maturation of regenerating myelinated axons. Neurofilament 68(NF68), also called Neurofilament Protein, Light Chain(NFL). It is one of the most abundant cytoskeletal components of the neuron. Mutations in this gene were reported as a cause for autosomal dominant Charcot-Marie-Tooth type 2E(CMT2E) linked to chromosome 8p21. NFL was identified repeatedly in both screenings and found to interact with Myotubularin-related 2 gene, MTMR2 in both Schwann cells and neurons.
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FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.