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SKU:PA1664
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-NFAT1 Antibody
Description Rabbit IgG polyclonal antibody for Nuclear factor of activated T-cells, cytoplasmic 2(NFATC2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-NFAT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1664)
Replacement Item This antibody may replace the following items: sc-13034|sc-7295|sc-23815|sc-7296|sc-136206|sc-1151|sc-13034-X|sc-23815-X|sc-514929-X|sc-514929|sc-7296-X|sc-7295-X|sc-1151-X from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Bovine

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human NFAT1(895-913aa KQEQNLDQTYLDDVNEIIR), identical to the related rat and mouse sequences.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name NFATC2
Protein Name Nuclear factor of activated T-cells, cytoplasmic 2(NF-ATc2/NFATc2)
Molecular Weight 100146 MW
Protein Function Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway. .
Tissue Specificity Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Isoform 1 is highly expressed in the small intestine, heart, testis, prostate, thymus, placenta and thyroid. Isoform 3 is highly expressed in stomach, uterus, placenta, trachea and thyroid. .
Sequence Similarities Contains 1 RHD (Rel-like) domain.
Subcellular Localization Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
Uniprot ID Q13469
Alternative Names Nuclear factor of activated T-cells, cytoplasmic 2;NF-ATc2;NFATc2;NFAT pre-existing subunit;NF-ATp;T-cell transcription factor NFAT1;NFATC2;NFAT1, NFATP;
Research Areas |signal transduction|signaling pathway|nuclear signaling|nfats| immunology|innate immunity|cytokines| epigenetics and nuclear signaling|nuclear signaling pathways|adaptive immunity|regulatory t cells|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Nuclear factor of activated T-cells, cytoplasmic 2(NF-ATc2/NFATc2)

NFATC2(Nuclear factor of activated T-cells, cytoplasmic 2),also known as NFATP or the "preexisting component" of NFAT, is present in the cytosolic fraction of unstimulated T cells, which is also a member of the nuclear factor of activated T cells(NFAT) family. The NFATC2 gene is mapped on 20q13.2. NFATC2 is highly homologous to NFATC1 over a limited domain which shows similarity to the Dorsal/Rel family but has a wider tissue distribution. Ectopic expression of NFATC2 inhibited the basal activity of the human CDK4 promoter. Additionally, both Calna-/- and Nfatc2 -/- mice had elevated protein levels of Cdk4, confirming a negative regulatory role for the calcineurin/NFAT pathway. NFATC2 controls myoblast fusion at a specific stage of myogenesis after the initial formation of a myotube and is necessary for further cell growth. Overexpression of NFATC2 promoted differentiation of osteoclast precursor cells into tartrate-resistant acid phosphatase-positive(TRAP-positive) multinucleated osteoclast-like cells even in the absence of RANKL.

Anti-NFAT1 Antibody Images

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Anti-NFAT1 Antibody
Anti-NFAT1 antibody, PA1664, Western blotting
All lanes: Anti NFAT1 (PA1664) at 0.5ug/ml
Lane 1: PANC Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: U87 Whole Cell Lysate at 40ug
Predicted bind size: 87KD
Observed bind size: 87KD
Anti-NFAT1 Antibody
Anti-NFAT1 antibody, PA1664, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Publications

Bai, Y., Li, Z.X., Wang, H.L., Lian, G.C., & Wang, Y. (2017). The protective effects of PCPA against monocrotaline-induced pulmonary arterial hypertension are mediated through the downregulation of NFAT-1 and NF-κB. International Journal of Molecular Medicine, 40(1), 155-163. Advance online publication. doi: 10.3892/ijmm.2017.3001

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to nfat1 antibody, nfatc2 antibody