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SKU:PB9150
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, WB
Price: $240.00
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Overview

Product Name Anti-NFkB p100/p52 Picoband™ Antibody
SKU/Catalog Number PB9150
Description Rabbit IgG polyclonal antibody for Nuclear factor NF-kappa-B p100 subunit(NFKB2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-NFkB p100/p52 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9150)
Replacement Item This antibody may replace the following items: sc-20231|sc-20233|sc-514564|sc-20231|sc-20232|sc-20233|sc-398820|sc-398820-X|sc-398821|sc-398821-X|sc-48800|sc-48800-X|sc-514564 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Bovine

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human NFkB p100/p52 recombinant protein (Position: M1-R340). Human NFkB p100/p52 shares 96% amino acid (aa) sequence identity with mouse NFkB p100/p52.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name MYD88
Protein Name Nuclear factor NF-kappa-B p100 subunit
Molecular Weight 33233 MW
Protein Function Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine. .
Tissue Specificity Ubiquitous. .
Sequence Similarities Contains 7 ANK repeats.
Subcellular Localization Cytoplasm .
Uniprot ID Q99836
Alternative Names Myeloid differentiation primary response protein MyD88;MYD88;
Research Areas NFKB2|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Nuclear factor NF-kappa-B p100 subunit

NFKB2, also known as nuclear factor NF-kappa-B p100 subunit, is a protein that in humans is encoded by the NFKB2 gene. It is mapped to 10q24.32. This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFKB). NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner.

Anti-NFkB p100/p52 Picoband™ Antibody Images

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Anti-NFkB p100/p52 Picoband™ Antibody
Anti-NFkB p100 Picoband antibody, PB9150-1.jpg
All lanes: Anti NFKBP100 (PB9150) at 0.5ug/ml
WB: Recombinant Human NFKBP100 Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
Anti-NFkB p100/p52 Picoband™ Antibody
Anti-NFkB p100 Picoband antibody, PB9150-2.jpg
All lanes: Anti NFKBP100 (PB9150) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: A549 Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 100KD
Observed bind size: 100KD
Anti-NFkB p100/p52 Picoband™ Antibody
Anti-NFkB p100 Picoband antibody, PB9150-3.JPG
IHC(P): Human Lung Cancer Tissue
Anti-NFkB p100/p52 Picoband™ Antibody
Anti-NFkB p100 Picoband antibody, PB9150-4.JPG
IHC(P): Human Mammary Cancer Tissue
Anti-NFkB p100/p52 Picoband™ Antibody
Anti-NFkB p100 Picoband antibody, PB9150-5.JPG
IHC(P): Mouse Intestine Tissue
Anti-NFkB p100/p52 Picoband™ Antibody
Anti-NFkB p100 Picoband antibody, PB9150-6.JPG
IHC(P): Rat Intestine Tissue
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Publications

Zhao Yj, Wang H, Liu X, Sun M, Kazuhiro H. Mol Med Rep. 2012 Oct;6(4):739-44. Doi: 10.3892/Mmr.2012.1007. Epub 2012 Jul 26. Protective Effects Of Glutamine In A Rat Model Of Endotoxemia.
Li Y, Chen Y, Zhang J, Zhu Jf, Liu Zj, Liang Sy, Sun K, Liao Wy, Gong Jp. Am J Surg. 2010 Jan;199(1):35-42. Doi: 10.1016/J.Amjsurg.2008.11.039. Protective Effect Of Glutamine-Enriched Early Enteral Nutrition On Intestinal Mucosal Barrier Injury Af...
Kan S, Zhou H, Jin C, Yang H. Int J Clin Exp Med. 2015 Mar 15;8(3):3258-70. Ecollection 2015. Effects Of Pdtc On Nf-??b Expression And Apoptosis In Rats With Severe Acute Pancreatitis-Associated Lung Injury.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is nfkb2 antibody