Anti-NFkB/NFKB1 p105/p50 Antibody Picoband™

NFKB1 antibody

Boster Bio Anti-NFkB/NFKB1 p105/p50 Antibody Picoband™ catalog # PB9149. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. Cited in 10 publication(s).

Product Info Summary

SKU: PB9149
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, ICC, WB

Product Name

Anti-NFkB/NFKB1 p105/p50 Antibody Picoband™

View all NFKB1 Antibodies

SKU/Catalog Number

PB9149

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NFkB/NFKB1 p105/p50 Antibody Picoband™ catalog # PB9149. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NFkB/NFKB1 p105/p50 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9149)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human NFkB/NFKB1 p105/p50 recombinant protein (Position: M1-Q360). Human NFkB p105/P50 shares 93% amino acid (aa) sequence identity with mouse NFkB p105/P50.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9149 is reactive to NFKB1 in Human

Applications

PB9149 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

50 kDa, 120 kDa

Calculated molecular weight

105.356kDa

Background of NFKB1

Nuclear factor NF-kappa-B p105 subunit, also called EBP-1 is a protein that in humans is encoded by the NFKB1 gene. By fluorescence in situ hybridization, the gene was assigned to human chromosome 4q24. NFKB1 is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NFKB1 proteins by p105 and generation of p50 by a cotranslational processing.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For NFKB1 (Source: Uniprot.org, NCBI)

Gene Name

NFKB1

Full Name

Nuclear factor NF-kappa-B p105 subunit

Weight

105.356kDa

Alternative Names

DKFZp686C01211; DNA binding factor KBF1; DNA-binding factor KBF1; EBP-1; KBF1; NF-kappaB; NF-kappa-B; NF-kappabeta; NFKB-p105; NFKB-p50; nuclear factor kappa-B DNA binding subunit; nuclear factor NF-kappa-B p105 subunit; nuclear factor NF-kappa-B p50 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 1MGC54151; p105; p50 NFKB1 CVID12, EBP-1, KBF1, NF-kB, NF-kB1, NF-kappa-B1, NF-kappaB, NF-kappabeta, NFKB-p105, NFKB-p50, NFkappaB nuclear factor kappa B subunit 1 nuclear factor NF-kappa-B p105 subunit|DNA-binding factor KBF1|nuclear factor NF-kappa-B p50 subunit|nuclear factor kappa-B DNA binding subunit|nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NFKB1, check out the NFKB1 Infographic

NFKB1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NFKB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9149 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Prophylactic effects of triptolide on colon cancer development in azoxymethane/dextran sulfate sodiuminduced mouse model

Upregulation of Toll‑like receptor 2 expression in colorectal cancer infected by human cytomegalovirus

MiR199b Suppresses Expression of Hypoxia-Inducible Factor 1α (HIF-1α) in Prostate Cancer Cells

Curcumin Derivative Cur20 Attenuated Cerebral Ischemic Injury by Antioxidant Effect and HIF-1α/VEGF/TFEB-Activated Angiogenesis

Effect of ammonium pyrrolidine dithiocarbamate (PDTC) on NF-κB activation and CYP2E1 content of rats with immunological liver injury

Breast cancer pathogenesis is linked to the intra-tumoral estrogen sulfotransferase (hSULT1E1) expressions regulated by cellular redox dependent Nrf-2/NF κ β interplay

Hydrogen Sulfide Mitigates Kidney Injury in High Fat Diet-Induced Obese Mice

HMGB1 is a key factor for tamoxifen resistance and has the potential to predict the efficacy of CDK4/6 inhibitors in breast cancer

The oncogenic role of NS5A of hepatitis C virus is mediated by up‐regulation of survivin gene expression in the hepatocellular cell through p53 and NF‐κB pathways

HMGB3 modulates ROS production via activating TLR cascade in Apostichopus japonicus

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1 Customer Q&As for Anti-NFkB/NFKB1 p105/p50 Antibody Picoband™

Question

We are currently using anti-NFkB p105/p50/NFKB1 antibody PB9149 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on primate tissues as well?

R. Thomas

Verified customer

Asked: 2017-07-27

Answer

The anti-NFkB p105/p50/NFKB1 antibody (PB9149) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-07-27

Order DetailsPrice
PB9149

100μg

$370
PB9149-10ug

10μg sample (liquid)

$99
PB9149-Biotin

100 μg Biotin conjugated

$570
PB9149-Cy3

100 μg Cy3 conjugated

$570
PB9149-Dylight488

100 μg Dylight488 conjugated

$570
PB9149-Dylight550

100 μg Dylight550 conjugated

$570
PB9149-Dylight594

100 μg Dylight594 conjugated

$570
PB9149-FITC

100 μg FITC conjugated

$570
PB9149-HRP

100 μg HRP conjugated

$570
PB9149-APC

100 μg APC conjugated

$670
PB9149-PE

100 μg PE conjugated

$670
PB9149-carrier-free

Carrier Free

$370

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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