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SKU:PB9149
Clonality:Polyclonal
Application:IHC-P, WB
Price: $240.00
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Overview

Product Name Anti-NFkB p105/P50 Picoband™ Antibody
Description Rabbit IgG polyclonal antibody for Nuclear factor NF-kappa-B p105 subunit(NFKB1) detection. Tested with WB, IHC-P in Human;Rat.
Cite This Product Anti-NFkB p105/P50 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9149)
Replacement Item This antibody may replace the following items: sc-23639|sc-134427|sc-20228|sc-20230 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Rat
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human NFkB p105/P50 recombinant protein (Position: M1-Q360). Human NFkB p105/P50 shares 93% amino acid (aa) sequence identity with mouse NFkB p105/P50.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name NFKB1
Protein Name Nuclear factor NF-kappa-B p105 subunit
Molecular Weight 105356 MW
Protein Function NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105. .
Sequence Similarities Contains 7 ANK repeats.
Subcellular Localization Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).
Uniprot ID P19838
Alternative Names Nuclear factor NF-kappa-B p105 subunit;DNA-binding factor KBF1;EBP-1;Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1;Nuclear factor NF-kappa-B p50 subunit;NFKB1;
Research Areas NFKB1|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Nuclear factor NF-kappa-B p105 subunit

Nuclear factor NF-kappa-B p105 subunit, also called EBP-1 is a protein that in humans is encoded by the NFKB1 gene. By fluorescence in situ hybridization, the gene was assigned to human chromosome 4q24. NFKB1 is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NFKB1 proteins by p105 and generation of p50 by a cotranslational processing.

Anti-NFkB p105/P50 Picoband™ Antibody Images

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Anti-NFkB p105/P50 Picoband™ Antibody
Anti-NFkB p105/P50 Picoband antibody, PB9149-1.jpg
All lanes: Anti NFKBP105 (PB9149) at 0.5ug/ml
WB: Recombinant Human NFKBP105 Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
Anti-NFkB p105/P50 Picoband™ Antibody
Anti-NFkB p105/P50 Picoband antibody, PB9149-2.jpg
All lanes: Anti NFKBP105 (PB9149) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: PC-12 Whole Cell Lysate at 40ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: A431 Whole Cell Lysate at 40ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Lane 6: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 50KD
Observed bind size: 50KD
Anti-NFkB p105/P50 Picoband™ Antibody
Anti-NFkB p105/P50 Picoband antibody, PB9149-3.JPG
IHC(P): Human Lung Cancer Tissue
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Publications

Leng Am, Liu T, Yang J, Cui Jf, Li Xh, Zhu Yn, Xiong T, Zhang G, Chen Y. Cell Biol Int. 2012 Oct 1;36(10):893-9. Doi: 10.1042/Cbi20110473. The Apoptotic Effect And Associated Signalling Of Hsp90 Inhibitor 17-Dmag In Hepatocellular Carcinoma Cells.
Zhao Yj, Wang H, Liu X, Sun M, Kazuhiro H. Mol Med Rep. 2012 Oct;6(4):739-44. Doi: 10.3892/Mmr.2012.1007. Epub 2012 Jul 26. Protective Effects Of Glutamine In A Rat Model Of Endotoxemia.
Li Y, Chen Y, Zhang J, Zhu Jf, Liu Zj, Liang Sy, Sun K, Liao Wy, Gong Jp. Am J Surg. 2010 Jan;199(1):35-42. Doi: 10.1016/J.Amjsurg.2008.11.039. Protective Effect Of Glutamine-Enriched Early Enteral Nutrition On Intestinal Mucosal Barrier Injury Af...
Kan S, Zhou H, Jin C, Yang H. Int J Clin Exp Med. 2015 Mar 15;8(3):3258-70. Ecollection 2015. Effects Of Pdtc On Nf-??b Expression And Apoptosis In Rats With Severe Acute Pancreatitis-Associated Lung Injury.
Xiao X, Gang Y, Wang H, Wang J, Zhao L, Xu L, Liu Z. Biochem Biophys Res Commun. 2015 Feb 6;457(2):221-6. Doi: 10.1016/J.Bbrc.2014.12.091. Epub 2014 Dec 27. Double-Stranded Rna Transcribed From Vector-Based Oligodeoxynucleotide Acts As Transcripti...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to ebp1 antibody, nf kappa b antibody, nfkappab antibody, nfkb p50 antibody, nfkb1 antibody