Anti-NG2 CSPG4 Rabbit Monoclonal Antibody

NG2/MCSP antibody

Boster Bio Anti-NG2 CSPG4 Rabbit Monoclonal Antibody catalog # M03394. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: M03394
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IHC, WB

Customers Who Bought This Also Bought

Product Name

Anti-NG2 CSPG4 Rabbit Monoclonal Antibody

View all NG2/MCSP Antibodies

SKU/Catalog Number

M03394

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-NG2 CSPG4 Rabbit Monoclonal Antibody catalog # M03394. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NG2 CSPG4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03394)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AOFG-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human NG2

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M03394 is reactive to CSPG4 in Human

Applications

M03394 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

50 kDa

Calculated molecular weight

250.537kDa

Background of NG2/MCSP

Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For CSPG4 (Source: Uniprot.org, NCBI)

Gene Name

CSPG4

Full Name

Chondroitin sulfate proteoglycan 4

Weight

250.537kDa

Alternative Names

AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8 CSPG4 CSPG4A, HMW-MAA, MCSP, MCSPG, MEL-CSPG, MSK16, NG2 chondroitin sulfate proteoglycan 4 chondroitin sulfate proteoglycan 4|chondroitin sulfate proteoglycan 4 (melanoma-associated)|chondroitin sulfate proteoglycan NG2|melanoma chondroitin sulfate proteoglycan|melanoma-associated chondroitin sulfate proteoglycan

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CSPG4, check out the CSPG4 Infographic

CSPG4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CSPG4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for M03394

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-NG2 CSPG4 Rabbit Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-NG2 CSPG4 Rabbit Monoclonal Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

3 Customer Q&As for Anti-NG2 CSPG4 Rabbit Monoclonal Antibody

Question

We are currently using anti-NG2 Rabbit Monoclonal antibody M03394 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?

L. Yang

Verified customer

Asked: 2020-01-15

Answer

The anti-NG2 Rabbit Monoclonal antibody (M03394) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-15

Question

Will anti-NG2 Rabbit Monoclonal antibody M03394 work on dog WB with tendon?

Verified Customer

Verified customer

Asked: 2018-05-25

Answer

Our lab technicians have not validated anti-NG2 Rabbit Monoclonal antibody M03394 on dog. You can run a BLAST between dog and the immunogen sequence of anti-NG2 Rabbit Monoclonal antibody M03394 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog tendon in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-05-25

Question

I was wanting to use your anti-NG2 Rabbit Monoclonal antibody for WB for human melanoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human melanoma identification?

Verified Customer

Verified customer

Asked: 2017-08-23

Answer

As indicated on the product datasheet, M03394 anti-NG2 Rabbit Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human melanoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-08-23

Order DetailsPrice
M03394

100μL

$370
M03394-30ul

30μL sample

$99
M03394-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
M03394
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.