Anti-NGF/NGF Beta Antibody Picoband™

beta-NGF antibody

Boster Bio Anti-NGF/NGF Beta Antibody Picoband™ catalog # A00341. Tested in ELISA, WB applications. This antibody reacts with Human. Cited in 5 publication(s).

Product Info Summary

SKU: A00341
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-NGF/NGF Beta Antibody Picoband™

View all beta-NGF Antibodies

SKU/Catalog Number

A00341

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NGF/NGF Beta Antibody Picoband™ catalog # A00341. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NGF/NGF Beta Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00341)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human NGF/NGF Beta recombinant protein (Position: S122-A241). Human NGF/NGF Beta shares 92.4% amino acid (aa) sequence identity with rat NGF/NGF Beta.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00341 is reactive to NGF in Human

Applications

A00341 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

13 kDa

Calculated molecular weight

26.959kDa

Background of beta-NGF

Nerve growth factor is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. The nucleotide sequence of human and mouse beta-NGF are very similar. The beta-subunits of nerve growth factor (NGFB) have been assigned to mouse chromosome 3 and human chromosome 1p22. The human gene for the beta subunit of nerve growth factor is located on the proximal short arm of chromosome 1. A mutation in the nerve growth factor beta gene (NGFB) causes loss of pain perception.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For NGF (Source: Uniprot.org, NCBI)

Gene Name

NGF

Full Name

Beta-nerve growth factor

Weight

26.959kDa

Superfamily

NGF-beta family

Alternative Names

Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428 NGF Beta-NGF, HSAN5B, NGF nerve growth factor beta-nerve growth factor|nerve growth factor (beta polypeptide)|nerve growth factor, beta subunit|pro-nerve growth factor long

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NGF, check out the NGF Infographic

NGF infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NGF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00341 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Transplantation of human umbilical cord blood mesenchymal stem cells to treat a rat model of traumatic brain injury

Effects of minocycline on the expression of NGF and HSP70 and its neuroprotection role following intracerebral hemorrhage in rats

Associations between proinflammatory cytokines in the synovial fluid and radiographic grading and pain-related scores in 47 consecutive patients with osteoarthritis of the knee

Huang S, Zhang X, Xu L, Li Q, Zhao Q. Exp Ther Med. 2014 Oct;8(4):1237-1240. Epub 2014 Jul 21. Expression Of Nerve Growth Factor In The Prostate Of Male Rats In Response To Chronic Stress And Sympathetic Denervation.

Luo Q, Liu W, Chen J, Wang M, Zeng W, Chen Z, Cheng A. Neural Regen Res. 2012 Feb 5;7(4):302-7. Doi: 10.3969/J.Issn.1673-5374.2012.04.011. Nerve Growth Factor And Inducible Nitric Oxide Synthase Expression In The Mesencephalon And Diencephalon, As...

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15 Customer Q&As for Anti-NGF/NGF Beta Antibody Picoband™

Question

I was wanting to use your anti-NGF/NGF Beta antibody for WB for human cartilage tissue on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cartilage tissue identification?

Verified Customer

Verified customer

Asked: 2020-03-27

Answer

You can see on the product datasheet, A00341 anti-NGF/NGF Beta antibody has been validated for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cartilage tissue in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-27

Question

We are currently using anti-NGF/NGF Beta antibody A00341 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-23

Answer

The anti-NGF/NGF Beta antibody (A00341) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-23

Question

Is there a BSA free version of anti-NGF/NGF Beta antibody A00341 available?

Verified Customer

Verified customer

Asked: 2020-02-25

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NGF/NGF Beta antibody A00341 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-02-25

Question

I am looking for to test anti-NGF/NGF Beta antibody A00341 on human cartilage tissue for research purposes, then I may be interested in using anti-NGF/NGF Beta antibody A00341 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-20

Answer

The products we sell, including anti-NGF/NGF Beta antibody A00341, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-20

Question

I see that the anti-NGF/NGF Beta antibody A00341 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-01-17

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-01-17

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cartilage tissue using anti-NGF/NGF Beta antibody A00341. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-13

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-13

Question

We have tried in the past anti-NGF/NGF Beta antibody for ELISA on brain in the past. I am using human, and We want to use the antibody for WB next. We are interested in examining brain as well as cartilage tissue in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-11-15

Answer

I viewed the website and datasheets of our anti-NGF/NGF Beta antibody and I see that A00341 has been validated on human in both ELISA and WB. Thus A00341 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-11-15

Question

Does anti-NGF/NGF Beta antibody A00341 work for WB with cartilage tissue?

Verified Customer

Verified customer

Asked: 2019-09-27

Answer

According to the expression profile of cartilage tissue, NGF is highly expressed in cartilage tissue. So, it is likely that anti-NGF/NGF Beta antibody A00341 will work for WB with cartilage tissue.

Boster Scientific Support

Answered: 2019-09-27

Question

Is a blocking peptide available for product anti-NGF/NGF Beta antibody (A00341)?

Verified Customer

Verified customer

Asked: 2019-09-03

Answer

We do provide the blocking peptide for product anti-NGF/NGF Beta antibody (A00341). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-09-03

Question

Can you help my question with product A00341, anti-NGF/NGF Beta antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-05-15

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00341 anti-NGF/NGF Beta antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-05-15

Question

We have seen staining in human leukocyte. Any tips? Is anti-NGF/NGF Beta antibody supposed to stain leukocyte positively?

Verified Customer

Verified customer

Asked: 2018-02-15

Answer

From literature leukocyte does express NGF. From Uniprot.org, NGF is expressed in cartilage tissue, brain, eye, leukocyte, among other tissues. Regarding which tissues have NGF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2374737
Eye, Pubmed ID: 15489334
Leukocyte, Pubmed ID: 2025430

Boster Scientific Support

Answered: 2018-02-15

Question

See attached the WB image, lot number and protocol we used for cartilage tissue using anti-NGF/NGF Beta antibody A00341. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-01-01

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-01-01

Question

Is this A00341 anti-NGF/NGF Beta antibody reactive to the isotypes of NGF?

C. Walker

Verified customer

Asked: 2016-11-02

Answer

The immunogen of A00341 anti-NGF/NGF Beta antibody is E. coli-derived human NGF/NGF Beta recombinant protein (Position: S122-A241). Human NGF/NGF Beta shares 92.4% amino acid (aa) sequence identity with rat NGF/NGF Beta. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2016-11-02

Question

We were satisfied with the WB result of your anti-NGF/NGF Beta antibody. However we have seen positive staining in brain secreted. using this antibody. Is that expected? Could you tell me where is NGF supposed to be expressed?

A. Roberts

Verified customer

Asked: 2016-10-26

Answer

From what I have seen in literature, brain does express NGF. Generally NGF expresses in secreted. Regarding which tissues have NGF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2374737
Eye, Pubmed ID: 15489334
Leukocyte, Pubmed ID: 2025430

Boster Scientific Support

Answered: 2016-10-26

Question

Would A00341 anti-NGF/NGF Beta antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

S. Anderson

Verified customer

Asked: 2014-09-22

Answer

You can see on the product datasheet, A00341 anti-NGF/NGF Beta antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-09-22

Order DetailsPrice
A00341

100μg

$370
A00341-10ug

10μg sample (liquid)

$99
A00341-Biotin

100 μg Biotin conjugated

$570
A00341-Cy3

100 μg Cy3 conjugated

$570
A00341-Dylight488

100 μg Dylight488 conjugated

$570
A00341-Dylight550

100 μg Dylight550 conjugated

$570
A00341-Dylight594

100 μg Dylight594 conjugated

$570
A00341-FITC

100 μg FITC conjugated

$570
A00341-HRP

100 μg HRP conjugated

$570
A00341-APC

100 μg APC conjugated

$670
A00341-PE

100 μg PE conjugated

$670
A00341-carrier-free

Carrier Free

$370

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A00341
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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