Product Info Summary
SKU: | A00341 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-NGF/NGF Beta Antibody Picoband™
SKU/Catalog Number
A00341
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NGF/NGF Beta Antibody Picoband™ catalog # A00341. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NGF/NGF Beta Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00341)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human NGF/NGF Beta recombinant protein (Position: S122-A241). Human NGF/NGF Beta shares 92.4% amino acid (aa) sequence identity with rat NGF/NGF Beta.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00341 is reactive to NGF in Human
Applications
A00341 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
13 kDa
Calculated molecular weight
26.959kDa
Background of beta-NGF
Nerve growth factor is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. The nucleotide sequence of human and mouse beta-NGF are very similar. The beta-subunits of nerve growth factor (NGFB) have been assigned to mouse chromosome 3 and human chromosome 1p22. The human gene for the beta subunit of nerve growth factor is located on the proximal short arm of chromosome 1. A mutation in the nerve growth factor beta gene (NGFB) causes loss of pain perception.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of NGF/NGF Beta using anti-NGF/NGF Beta antibody (A00341).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Recombinant Human NGF/NGF Beta Protein 0.5ng
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NGF/NGF Beta antigen affinity purified polyclonal antibody (Catalog # A00341) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NGF/NGF Beta at approximately 13KD. The expected band size for NGF/NGF Beta is at 13KD.
Protein Target Info & Infographic
Gene/Protein Information For NGF (Source: Uniprot.org, NCBI)
Gene Name
NGF
Full Name
Beta-nerve growth factor
Weight
26.959kDa
Superfamily
NGF-beta family
Alternative Names
Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428 NGF Beta-NGF, HSAN5B, NGF nerve growth factor beta-nerve growth factor|nerve growth factor (beta polypeptide)|nerve growth factor, beta subunit|pro-nerve growth factor long
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NGF, check out the NGF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NGF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NGF/NGF Beta Antibody Picoband™ (A00341)
Hello CJ!
A00341 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Transplantation of human umbilical cord blood mesenchymal stem cells to treat a rat model of traumatic brain injury
Effects of minocycline on the expression of NGF and HSP70 and its neuroprotection role following intracerebral hemorrhage in rats
Associations between proinflammatory cytokines in the synovial fluid and radiographic grading and pain-related scores in 47 consecutive patients with osteoarthritis of the knee
Huang S, Zhang X, Xu L, Li Q, Zhao Q. Exp Ther Med. 2014 Oct;8(4):1237-1240. Epub 2014 Jul 21. Expression Of Nerve Growth Factor In The Prostate Of Male Rats In Response To Chronic Stress And Sympathetic Denervation.
Luo Q, Liu W, Chen J, Wang M, Zeng W, Chen Z, Cheng A. Neural Regen Res. 2012 Feb 5;7(4):302-7. Doi: 10.3969/J.Issn.1673-5374.2012.04.011. Nerve Growth Factor And Inducible Nitric Oxide Synthase Expression In The Mesencephalon And Diencephalon, As...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
15 Customer Q&As for Anti-NGF/NGF Beta Antibody Picoband™
Question
I was wanting to use your anti-NGF/NGF Beta antibody for WB for human cartilage tissue on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cartilage tissue identification?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
You can see on the product datasheet, A00341 anti-NGF/NGF Beta antibody has been validated for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human cartilage tissue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-27
Question
We are currently using anti-NGF/NGF Beta antibody A00341 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
The anti-NGF/NGF Beta antibody (A00341) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-23
Question
Is there a BSA free version of anti-NGF/NGF Beta antibody A00341 available?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NGF/NGF Beta antibody A00341 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-25
Question
I am looking for to test anti-NGF/NGF Beta antibody A00341 on human cartilage tissue for research purposes, then I may be interested in using anti-NGF/NGF Beta antibody A00341 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
The products we sell, including anti-NGF/NGF Beta antibody A00341, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-20
Question
I see that the anti-NGF/NGF Beta antibody A00341 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-17
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cartilage tissue using anti-NGF/NGF Beta antibody A00341. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-13
Question
We have tried in the past anti-NGF/NGF Beta antibody for ELISA on brain in the past. I am using human, and We want to use the antibody for WB next. We are interested in examining brain as well as cartilage tissue in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
I viewed the website and datasheets of our anti-NGF/NGF Beta antibody and I see that A00341 has been validated on human in both ELISA and WB. Thus A00341 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-15
Question
Does anti-NGF/NGF Beta antibody A00341 work for WB with cartilage tissue?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
According to the expression profile of cartilage tissue, NGF is highly expressed in cartilage tissue. So, it is likely that anti-NGF/NGF Beta antibody A00341 will work for WB with cartilage tissue.
Boster Scientific Support
Answered: 2019-09-27
Question
Is a blocking peptide available for product anti-NGF/NGF Beta antibody (A00341)?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
We do provide the blocking peptide for product anti-NGF/NGF Beta antibody (A00341). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-03
Question
Can you help my question with product A00341, anti-NGF/NGF Beta antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00341 anti-NGF/NGF Beta antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-15
Question
We have seen staining in human leukocyte. Any tips? Is anti-NGF/NGF Beta antibody supposed to stain leukocyte positively?
Verified Customer
Verified customer
Asked: 2018-02-15
Answer
From literature leukocyte does express NGF. From Uniprot.org, NGF is expressed in cartilage tissue, brain, eye, leukocyte, among other tissues. Regarding which tissues have NGF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2374737
Eye, Pubmed ID: 15489334
Leukocyte, Pubmed ID: 2025430
Boster Scientific Support
Answered: 2018-02-15
Question
See attached the WB image, lot number and protocol we used for cartilage tissue using anti-NGF/NGF Beta antibody A00341. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-01-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-01
Question
Is this A00341 anti-NGF/NGF Beta antibody reactive to the isotypes of NGF?
C. Walker
Verified customer
Asked: 2016-11-02
Answer
The immunogen of A00341 anti-NGF/NGF Beta antibody is E. coli-derived human NGF/NGF Beta recombinant protein (Position: S122-A241). Human NGF/NGF Beta shares 92.4% amino acid (aa) sequence identity with rat NGF/NGF Beta. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-11-02
Question
We were satisfied with the WB result of your anti-NGF/NGF Beta antibody. However we have seen positive staining in brain secreted. using this antibody. Is that expected? Could you tell me where is NGF supposed to be expressed?
A. Roberts
Verified customer
Asked: 2016-10-26
Answer
From what I have seen in literature, brain does express NGF. Generally NGF expresses in secreted. Regarding which tissues have NGF expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 2374737
Eye, Pubmed ID: 15489334
Leukocyte, Pubmed ID: 2025430
Boster Scientific Support
Answered: 2016-10-26
Question
Would A00341 anti-NGF/NGF Beta antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Anderson
Verified customer
Asked: 2014-09-22
Answer
You can see on the product datasheet, A00341 anti-NGF/NGF Beta antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-09-22