Anti-NKCC1/SLC12A2 Antibody

NKCC1/SLC12A2 antibody

Boster Bio Anti-NKCC1/SLC12A2 Antibody catalog # PA2169. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA2169
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, WB

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Product Name

Anti-NKCC1/SLC12A2 Antibody

View all NKCC1/SLC12A2 Antibodies

SKU/Catalog Number

PA2169

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NKCC1/SLC12A2 Antibody catalog # PA2169. Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NKCC1/SLC12A2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2169)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human NKCC1, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2169 is reactive to SLC12A2 in Human, Mouse, Rat

Applications

PA2169 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

200 kDa

Calculated molecular weight

131.447kDa

Background of NKCC1/SLC12A2

Solute carrier family 12 (sodium/potassium/chloride transporters), member 2, also known as NKCC1, is widely distributed throughout the body, especially in organs that secrete fluids, called exocrine glands. By fluorescence in situ hybridization, this gene is mapped to chromosome 5q23.3. The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For SLC12A2 (Source: Uniprot.org, NCBI)

Gene Name

SLC12A2

Full Name

Solute carrier family 12 member 2

Weight

131.447kDa

Superfamily

SLC12A transporter family

Alternative Names

Basolateral Na-K-Cl symporter; BSC; BSC2; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1; NKCC1; NKCC1MGC104233; SLC12A2; solute carrier family 12 (sodium/potassium/chloride transporters), member 2; solute carrier family 12 member 2 SLC12A2 BSC, BSC2, KILQS, NKCC1, PPP1R141 solute carrier family 12 member 2 solute carrier family 12 member 2|basolateral Na-K-Cl symporter|bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1|bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2|protein phosphatase 1, regulatory subunit 141|solute carrier family 12 (sodium/potassium/chloride transporter), member 2|solute carrier family 12 (sodium/potassium/chloride transporters), member 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SLC12A2, check out the SLC12A2 Infographic

SLC12A2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SLC12A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-NKCC1/SLC12A2 Antibody

Question

Is this PA2169 anti-NKCC1/SLC12A2 antibody reactive to the isotypes of SLC12A2?

Verified Customer

Verified customer

Asked: 2019-06-21

Answer

The immunogen of PA2169 anti-NKCC1/SLC12A2 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human NKCC1(223-241aa RIDHYRHTAAQLGEKLLRP), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-06-21

Question

Does anti-NKCC1/SLC12A2 antibody PA2169 work on monkey WB with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2019-05-27

Answer

Our lab technicians have not validated anti-NKCC1/SLC12A2 antibody PA2169 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-NKCC1/SLC12A2 antibody PA2169 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cervix carcinoma in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-27

Question

We are currently using anti-NKCC1/SLC12A2 antibody PA2169 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?

P. Dhar

Verified customer

Asked: 2018-02-28

Answer

The anti-NKCC1/SLC12A2 antibody (PA2169) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-02-28

Question

See attached the WB image, lot number and protocol we used for cervix carcinoma using anti-NKCC1/SLC12A2 antibody PA2169. Please let me know if you require anything else.

C. Wu

Verified customer

Asked: 2016-11-30

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-11-30

Question

My question regarding product PA2169, anti-NKCC1/SLC12A2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

M. Zhang

Verified customer

Asked: 2014-11-18

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2169 anti-NKCC1/SLC12A2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-11-18

Order DetailsPrice
PA2169

100μg

$370
PA2169-10ug

10μg sample (liquid)

$99
PA2169-Biotin

100 μg Biotin conjugated

$570
PA2169-Cy3

100 μg Cy3 conjugated

$570
PA2169-Dylight488

100 μg Dylight488 conjugated

$570
PA2169-Dylight550

100 μg Dylight550 conjugated

$570
PA2169-Dylight594

100 μg Dylight594 conjugated

$570
PA2169-FITC

100 μg FITC conjugated

$570
PA2169-HRP

100 μg HRP conjugated

$570
PA2169-APC

100 μg APC conjugated

$670
PA2169-PE

100 μg PE conjugated

$670
PA2169-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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