Anti-NKG2D/KLRK1 Antibody Picoband™

NKG2D/CD314 antibody

Boster Bio Anti-NKG2D/KLRK1 Antibody Picoband™ catalog # A00661-1. Tested in WB applications. This antibody reacts with Mouse, Rat.

Product Info Summary

SKU: A00661-1
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-NKG2D/KLRK1 Antibody Picoband™

View all NKG2D/CD314 Antibodies

SKU/Catalog Number

A00661-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NKG2D/KLRK1 Antibody Picoband™ catalog # A00661-1. Tested in WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NKG2D/KLRK1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00661-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human NKG2D, which shares 76.7% amino acid (aa) sequence identity with both mouse and rat NKG2D.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00661-1 is reactive to KLRK1 in Mouse, Rat

Applications

A00661-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

35 kDa

Calculated molecular weight

25.274kDa

Background of NKG2D/CD314

NKG2D is encoded by KLRK1 gene which is located in the NK-gene complex (NKC) situated on and chromosome 12 in humans. Natural killer (NK) cells are lymphocytes that can mediate lysis of certain tumor cells and virus-infected cells without previous activation. They can also regulate specific humoral and cell-mediated immunity. NK cells preferentially express several calcium-dependent (C-type) lectins, which have been implicated in the regulation of NK cell function. The NKG2 gene family is located within the NK complex, a region that contains several C-type lectin genes preferentially expressed in NK cells. This gene encodes a member of the NKG2 family. The encoded transmembrane protein is characterized by a type II membrane orientation (has an extracellular C terminus) and the presence of a C-type lectin domain. It binds to a diverse family of ligands that include MHC class I chain-related A and B proteins and UL-16 binding proteins, where ligand-receptor interactions can result in the activation of NK and T cells. The surface expression of these ligands is important for the recognition of stressed cells by the immune system, and thus this protein and its ligands are therapeutic targets for the treatment of immune diseases and cancers. Read-through transcription exists between this gene and the upstream KLRC4 (killer cell lectin-like receptor subfamily C, member 4) family member in the same cluster.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For KLRK1 (Source: Uniprot.org, NCBI)

Gene Name

KLRK1

Full Name

NKG2-D type II integral membrane protein

Weight

25.274kDa

Alternative Names

CD314 antigen; CD314; D12S2489E; FLJ17759; FLJ75772; Killer cell lectin-like receptor subfamily K member 1; killer cell lectin-like receptor subfamily K, member 1; KLR; KLRK1; NK cell receptor D; NKG2-D type II integral membrane protein; NKG2D; NKG2-D; NKG2-D-activating NK receptor; NKG2DDNA segment on chromosome 12 (unique) 2489 expressed sequence KLRK1 CD314, D12S2489E, KLR, NKG2-D, NKG2D killer cell lectin like receptor K1 NKG2-D type II integral membrane protein|NK cell receptor D|NKG2-D-activating NK receptor|killer cell lectin-like receptor subfamily K, member 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on KLRK1, check out the KLRK1 Infographic

KLRK1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for KLRK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-NKG2D/KLRK1 Antibody Picoband™

Question

Is a blocking peptide available for product anti-NKG2D/KLRK1 antibody (A00661-1)?

Verified Customer

Verified customer

Asked: 2019-11-29

Answer

We do provide the blocking peptide for product anti-NKG2D/KLRK1 antibody (A00661-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-11-29

Question

Is this A00661-1 anti-NKG2D/KLRK1 antibody reactive to the isotypes of KLRK1?

Verified Customer

Verified customer

Asked: 2019-05-31

Answer

The immunogen of A00661-1 anti-NKG2D/KLRK1 antibody is A synthetic peptide corresponding to a sequence of human NKG2D (YQFFDESKNWYESQASCMSQNASLLKVYSKEDQDLLKLVKSYH). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-05-31

Question

We are currently using anti-NKG2D/KLRK1 antibody A00661-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2018-11-23

Answer

The anti-NKG2D/KLRK1 antibody (A00661-1) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-11-23

Question

Would A00661-1 anti-NKG2D/KLRK1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-03-19

Answer

You can see on the product datasheet, A00661-1 anti-NKG2D/KLRK1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-03-19

Question

I see that the anti-NKG2D/KLRK1 antibody A00661-1 works with WB, what is the protocol used to produce the result images on the product page?

J. Lewis

Verified customer

Asked: 2017-01-12

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-01-12

Order DetailsPrice
A00661-1

100μg

$370
A00661-1-10ug

10μg sample (liquid)

$99
A00661-1-Biotin

100 μg Biotin conjugated

$570
A00661-1-Cy3

100 μg Cy3 conjugated

$570
A00661-1-Dylight488

100 μg Dylight488 conjugated

$570
A00661-1-Dylight550

100 μg Dylight550 conjugated

$570
A00661-1-Dylight594

100 μg Dylight594 conjugated

$570
A00661-1-FITC

100 μg FITC conjugated

$570
A00661-1-HRP

100 μg HRP conjugated

$570
A00661-1-APC

100 μg APC conjugated

$670
A00661-1-PE

100 μg PE conjugated

$670
A00661-1-carrier-free

Carrier Free

$370

More conjugates/formats

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A00661-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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