Product Info Summary
SKU: | A08603 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-NMBR/Neuromedin B R Antibody
View all Neuromedin BR/NMBR Antibodies
SKU/Catalog Number
A08603
Size
100μl
Form
Liquid
Description
Boster Bio Anti-NMBR/Neuromedin B R Antibody catalog # A08603. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NMBR/Neuromedin B R Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08603)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
2G2
Isotype
IgG
Immunogen
Synthetic Peptide
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A08603 is reactive to NMBR in Human, Mouse, Rat
Applications
A08603 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
43.435kDa
Background of Neuromedin BR/NMBR
APC11 is also known as Anaphase promoting complex subunit 11, APC11, Cyclosome subunit 11, Hepatocellular carcinoma associated RING finger protein, and HSPC214. APC11 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC11 may function to recruit the E2 ubiquitin-conjugating enzymes to the complex. APC11 interacts with the cullin domain of ANAPC2 and also interacts with UBE2D2. APC11 shows both a cytoplasmic and nuclear localization. APC11 is expressed at high levels in skeletal muscle and heart; in moderate levels in brain, kidney, and liver; and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte. APC11 is a member of the RING-type zinc finger family and is auto-ubiquitinylated.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:1000-2000
IHC, 1:200-500
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of 1) Human Brain Tissue, 2) Rat Brain Tissue using NMBR Polyclonal antibody.
Click image to see more details
Immunohistochemistry (IHC) analysis of paraffin-embedded Rat Brain Tissue using NMBR Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For NMBR (Source: Uniprot.org, NCBI)
Gene Name
NMBR
Full Name
Neuromedin-B receptor
Weight
43.435kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Neuromedin B R; neuromedin B receptor; Neuromedin BR; neuromedin-B receptor; Neuromedin-B-preferring bombesin receptor; NMBR; NMB-R NMBR BB1, BB1R, NMB-R neuromedin B receptor neuromedin-B receptor|bombesin receptor 1|epididymis secretory sperm binding protein Li 185a|epididymis tissue protein Li 185a|neuromedin-B-preferring bombesin receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NMBR, check out the NMBR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NMBR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NMBR/Neuromedin B R Antibody (A08603)
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No publications found for A08603
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-NMBR/Neuromedin B R Antibody
Question
Does anti-NMBR/Neuromedin B R antibody A08603 work on horse WB with epididymis?
R. Parker
Verified customer
Asked: 2020-05-05
Answer
Our lab technicians have not tested anti-NMBR/Neuromedin B R antibody A08603 on horse. You can run a BLAST between horse and the immunogen sequence of anti-NMBR/Neuromedin B R antibody A08603 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse epididymis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-05
Question
I would like to test anti-NMBR/Neuromedin B R antibody A08603 on mouse nucleus accumbens for research purposes, then I may be interested in using anti-NMBR/Neuromedin B R antibody A08603 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
The products we sell, including anti-NMBR/Neuromedin B R antibody A08603, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-10
Question
Will A08603 anti-NMBR/Neuromedin B R antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
As indicated on the product datasheet, A08603 anti-NMBR/Neuromedin B R antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-18
Question
We are currently using anti-NMBR/Neuromedin B R antibody A08603 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
The anti-NMBR/Neuromedin B R antibody (A08603) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-09
Question
Do you have a BSA free version of anti-NMBR/Neuromedin B R antibody A08603 available?
B. Gonzalez
Verified customer
Asked: 2019-05-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NMBR/Neuromedin B R antibody A08603 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-22
Question
Is this A08603 anti-NMBR/Neuromedin B R antibody reactive to the isotypes of NMBR?
Verified Customer
Verified customer
Asked: 2018-07-26
Answer
The immunogen of A08603 anti-NMBR/Neuromedin B R antibody is Synthetic Peptide. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-26
Question
I see that the anti-NMBR/Neuromedin B R antibody A08603 works with WB, what is the protocol used to produce the result images on the product page?
G. Wu
Verified customer
Asked: 2013-08-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-08-27