SKU PA1060
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications IHC, WB

Overview

Product Name Anti-Nogo A/RTN4 Antibody
SKU/Catalog Number PA1060
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Reticulon-4(RTN4) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-Nogo A/RTN4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1060)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Nogo A(1170-1192aa NKNVKDAMAKIQAKIPGLKRKAE), different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Images And Assay Conditions

/antibody/pa1060 1 WB anti nogo a antibody.jpg

Anti-Nogo A antibody, PA1060, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Brain Tissue Lysate

/antibody/pa1060 2 IHC anti nogo a antibody.jpg

Anti-Nogo A antibody, PA1060, IHC(P)
IHC(P): Rat Brain Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q9NQC3
Gene Name RTN4
Protein Name Reticulon-4
Tissue Specificity Isoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis- specific.
Alternative Names Reticulon-4;Foocen;Neurite outgrowth inhibitor;Nogo protein;Neuroendocrine-specific protein;NSP;Neuroendocrine-specific protein C homolog;RTN-x;Reticulon-5;RTN4;KIAA0886, NOGO;My043, SP1507;
Subcellular Localization Endoplasmic reticulum membrane; Multi-pass membrane protein. Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains.
Molecular Weight 129931 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity). Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing. .
Research Areas Neurology Process, Neuroscience

*You can search these to find other products in these research areas.
Background Human neurite outgrowth inhibitor(NOGO) cDNAs encodes 3 splice variants: NOGOA, NOGOB and NOGOC. The longest cDNA, designated NOGOA, has an open reading frame of 1192 amino acids. It is a potent inhibitor of neurite growth and an IN-1 antigen produced by oligodendrocytes, and may allow the generation of new reagents to enhance CNS regeneration and plasticity. Nogo-A, a member of the Reticulon family, is expressed by oligodendrocytes and associates primarily with the endoplasmic reticulum. The acidic amino terminus of Nogo-A is detected at the cytosolic face of cellular membranes and may contribute to inhibition of axon regeneration at sites of oligodendrocyte injury. A multivalent form of the N terminus of Nogo-A affects the morphology of both neurons and other cell types.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Order Product (PA1060)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1054 $280
100ug+Free Biotin Secondary BA1003 $280

USD $280

Ships in 7-10 business days.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

We promise all of our products perform as described in datasheets.

Write a review for PA1060

Publications

Transplantation of mesenchymal stem cells promotes the functional recovery of the central nervous system following cerebral ischemia by inhibiting myelin-associated inhibitor expression and neural apoptosis
Methylprednisolone inhibits Nogo-A protein expression after acute spinal cord injury
Expression of Nogo-A on the retina in rat model with chronic ocular hypertension
Quan-Cheng KanHui-Jun ZhangYuan ZhangXing LiYu-Ming XuRodolfo ThomeMing-Liang ZhangNan LiuYao-Juan ChuGuang-Xian ZhangEmail authorLi Zhu Neurobiol (2016). doi:10.1007/s12035-016-0333-1
Nie Qz, Liu Zl, Sha Q, Gao Dw. Int J Ophthalmol. 2010;3(2):112-3. Doi: 10.3980/J.Issn.2222-3959.2010.02.04. Epub 2010 Jun 18. Expression Of Nogo-A On The Retina In Rat Model With Chronic Ocular Hypertension.
Fu Z, Lu H, Jiang J, Jiang H, Zhang Z. Neural Regen Res. 2013 Feb 15;8(5):404-9. Doi: 10.3969/J.Issn.1673-5374.2013.05.003. Methylprednisolone Inhibits Nogo-A Protein Expression After Acute Spinal Cord Injury.
Wang B, Xiao Z, Chen B, Han J, Gao Y, Zhang J, Zhao W, Wang X, Dai J. Plos One. 2008 Mar 26;3(3):E1856. Doi: 10.1371/Journal.Pone.0001856. Nogo-66 Promotes The Differentiation Of Neural Progenitors Into Astroglial Lineage Cells Through Mtor-Stat3 ...
Wang L, Zhao H, Fan Yp, Gong Hy, Li M, Qi F, Liu Y. Chin J Integr Med. 2010 Apr;16(2):167-72. Doi: 10.1007/S11655-010-0167-Z. Epub 2010 May 16. Research On The Mechanism Of Zuogui Pill And Yougui Pill In Promoting Axonal Regeneration In Model Rats...

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to nogo a antibody, nogo antibody, nogo-a antibody