Data & Images
|Product Name||Anti-Notch1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Neurogenic locus notch homolog protein 1(NOTCH1) detection. Tested with WB in Human.|
|Cite This Product||Anti-Notch1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1215)|
|Replacement Item||This antibody may replace the following items: sc-164599|sc-164600|sc-23299|sc-23301|sc-23304|sc-32745|sc-373891|sc-373944|sc-376403|sc-6014|sc-6014-R|sc-9170 from Santa Cruz Biotechnology.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human Notch1(2487-2506aa QHSYSSPVDNTPSHQLQVPE).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Neurogenic locus notch homolog protein 1(Notch 1/hN1)|
|Molecular Weight||272505 MW|
|Protein Function||Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO). .|
|Tissue Specificity||In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.|
|Sequence Similarities||Belongs to the NOTCH family.|
|Subcellular Localization||Cell membrane ; Single-pass type I membrane protein .|
|Alternative Names||Neurogenic locus notch homolog protein 1;Notch 1;hN1;Translocation-associated notch protein TAN-1;Notch 1 extracellular truncation;NEXT;Notch 1 intracellular domain;NICD;NOTCH1;TAN1;|
|Research Areas|||neuroscience|neurology process|notch pathway| epigenetics and nuclear signaling|transcription|other factors| stem cells|signaling pathways|notch|surface molecules|nuclear|neural stem cells|intracellular|hematopoietic progenitors| cardiovascular|heart|cardiogenesis|transcription factors/regulators|neural crest stem cells| developmental biology|embryogenesis|somatogenesis||
Background for Neurogenic locus notch homolog protein 1(Notch 1/hN1)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Notch1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Notch1 Antibody Images
Click the images to enlarge.
All lanes: Anti Notch1 (PA1215) at 0.5ug/ml
Lane 1: MCF-7 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 272KD
Observed bind size: 272KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,