Anti-NADPH oxidase 1 NOX1 Antibody

NOX1 antibody

Boster Bio Anti-NADPH oxidase 1 NOX1 Antibody catalog # PA1739. Tested in IHC, WB applications. This antibody reacts with Human. Cited in 3 publication(s).

Product Info Summary

SKU: PA1739
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-NADPH oxidase 1 NOX1 Antibody

View all NOX1 Antibodies

SKU/Catalog Number

PA1739

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NADPH oxidase 1 NOX1 Antibody catalog # PA1739. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NADPH oxidase 1 NOX1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1739)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human NOX1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1739 is reactive to NOX1 in Human

Applications

PA1739 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

65 kDa

Calculated molecular weight

64.871kDa

Background of NOX1

NOX1 (NADPH OXIDASE 1), also known as NOH1, MOX1 or GP91-2, is an enzyme that in humans is encoded by the NOX1 gene. It is also a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. The NOX1 gene is mapped to Xq22.1. NOX1 was expressed in colon, prostate, uterus, and vascular smooth muscle, but not in peripheral blood leukocytes. The deduced 564-amino acid NOX1 protein, which is 58% identical to CYBB, contains 6 membrane-spanning regions, conserved flavin and pyridine nucleotide-binding sites, and histidines possibly involved in heme ligation. Overexpression of MOX1 in NIH 3T3 cells increased superoxide generation and cell growth. Cells expressing MOX1 had a transformed appearance, showed anchorage-independent growth, and produced tumors in athymic mice. Disruption of either Nox1 or Nox2 significantly delayed progression of motor neuron disease in these mice. However, 50% survival rates were enhanced significantly more by Nox2 deletion than Nox1 deletion.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For NOX1 (Source: Uniprot.org, NCBI)

Gene Name

NOX1

Full Name

NADPH oxidase 1

Weight

64.871kDa

Alternative Names

EC 1.6.3; GP91-2; Mitogenic oxidase 1; MOX-1; MOX1NADH/NADPH mitogenic oxidase subunit P65-MOX; NADPH oxidase 1; NADPH oxidase homolog-1; NOH1; NOH1mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating); NOH-1NADPH oxidase 1 variant NOH-1L; NOX1; NOX-1 NOX1 GP91-2, MOX1, NOH-1, NOH1 NADPH oxidase 1 NADPH oxidase 1|NADH/NADPH mitogenic oxidase subunit P65-MOX|NADPH oxidase homolog-1|mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating)|mitogenic oxidase 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NOX1, check out the NOX1 Infographic

NOX1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NOX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1739 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Deficiency of NOX1 or NOX4 Prevents Liver Inflammation and Fibrosis in Mice through Inhibition of Hepatic Stellate Cell Activation

Gu Y, Gong Y, Zhang H, Dong X, Zhao T, Burczynski Fj, Wang G, Sun S, Zhu B, Han W, Wang H, Li P. Biochem Cell Biol. 2013 Jun;91(3):187-93. Doi: 10.1139/Bcb-2012-0087. Epub 2012 Dec 13. Regulation Of Transforming Growth Factor Beta 1 Gene Expressio...

Wang Y, Wang Y, Liu D, Wang W, Zhao H, Wang M, Yin H. Carbohydr Polym. 2015 Jul 10;125:135-45. Doi: 10.1016/J.Carbpol.2015.02.012. Epub 2015 Feb 19. Cordyceps Sinensis Polysaccharide Inhibits Pdgf-Bb-Induced Inflammation And Ros Production In Huma...

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5 Customer Q&As for Anti-NADPH oxidase 1 NOX1 Antibody

Question

Will anti-NOX1 antibody PA1739 work for WB with colon epithelium?

Verified Customer

Verified customer

Asked: 2019-06-21

Answer

According to the expression profile of colon epithelium, NOX1 is highly expressed in colon epithelium. So, it is likely that anti-NOX1 antibody PA1739 will work for WB with colon epithelium.

Boster Scientific Support

Answered: 2019-06-21

Question

Does anti-NOX1 antibody PA1739 work on goat IHC with colon?

Verified Customer

Verified customer

Asked: 2018-01-12

Answer

Our lab technicians have not validated anti-NOX1 antibody PA1739 on goat. You can run a BLAST between goat and the immunogen sequence of anti-NOX1 antibody PA1739 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat colon in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-01-12

Question

Is there a BSA free version of anti-NOX1 antibody PA1739 available?

Verified Customer

Verified customer

Asked: 2017-07-25

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-NOX1 antibody PA1739 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-07-25

Question

We are currently using anti-NOX1 antibody PA1739 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on zebrafish tissues as well?

S. Walker

Verified customer

Asked: 2016-09-27

Answer

The anti-NOX1 antibody (PA1739) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-09-27

Question

See attached the WB image, lot number and protocol we used for colon epithelium using anti-NOX1 antibody PA1739. Please let me know if you require anything else.

Z. Jones

Verified customer

Asked: 2013-05-14

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-05-14

Order DetailsPrice
PA1739

100μg

$370
PA1739-10ug

10μg sample (liquid)

$99
PA1739-Biotin

100 μg Biotin conjugated

$570
PA1739-Cy3

100 μg Cy3 conjugated

$570
PA1739-Dylight488

100 μg Dylight488 conjugated

$570
PA1739-Dylight550

100 μg Dylight550 conjugated

$570
PA1739-Dylight594

100 μg Dylight594 conjugated

$570
PA1739-FITC

100 μg FITC conjugated

$570
PA1739-HRP

100 μg HRP conjugated

$570
PA1739-APC

100 μg APC conjugated

$670
PA1739-PE

100 μg PE conjugated

$670
PA1739-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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