Data & Images
|Product Name||Anti-NOX1 Antibody|
|Description||Rabbit IgG polyclonal antibody for NADPH oxidase 1(NOX1) detection. Tested with WB, IHC-P in Human.|
|Cite This Product||Anti-NOX1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1739)|
|Replacement Item||This antibody may replace the following items: sc-10183|sc-134389|sc-25545|sc-5819|sc-5821 from Santa Cruz Biotechnology.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human NOX1(354-374aa, HIRAAGDWTENLIRAFEQQYS).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||NADPH oxidase 1(NOX-1)|
|Molecular Weight||64871 MW|
|Protein Function||NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain.|
|Tissue Specificity||NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH- 1S is detected only in colon and colon carcinoma cells.|
|Sequence Similarities||Contains 1 FAD-binding FR-type domain.|
|Subcellular Localization||Cell projection, invadopodium membrane ; Multi-pass membrane protein .|
|Alternative Names||NADPH oxidase 1;NOX-1;1.-.-.-;Mitogenic oxidase 1;MOX-1;NADH/NADPH mitogenic oxidase subunit P65-MOX;NOH-1;NOX1;MOX1, NOH1;|
|Research Areas|||immunology|adaptive immunity|t cells|cytotoxic cells| epigenetics and nuclear signaling|dna / rna|dna damage & repair|dna damage response| signal transduction|metabolism|plasma membrane|channels| cell biology|other antibodies|oxidative stress| metabolism|pathways and processes|metabolic signaling pathways|nucleotide metabolism|redox metabolism|types of disease|cancer||
Background for NADPH oxidase 1(NOX-1)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-NOX1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-NOX1 Antibody Images
Click the images to enlarge.
IHC(P): Human Lung Cancer Tissue
All lanes: Anti NOX1 (PA1739) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 65KD
Observed bind size: 65KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,