Product Info Summary
SKU: | PA1667 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-NOX2/gp91phox/CYBB Antibody
SKU/Catalog Number
PA1667
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-NOX2/gp91phox/CYBB Antibody catalog # PA1667. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NOX2/gp91phox/CYBB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1667)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human NOX2, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1667 is reactive to CYBB in Human, Mouse, Rat
Applications
PA1667 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
65 kDa
Calculated molecular weight
65.336kDa
Background of CYBB/NOX2
NOX2 (NADPH OXIDASE 2), also called CYBB (CYTOCHROME b (-245), BETA SUBUNIT), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein.NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. It is mapped on Xp11.4. NOX2 is a heterodimer composed of an alpha chain of relative molecular mass 23 kD and a beta chain of 76 to 82 kD. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and that reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes. As a major player in innate immune responses in neutrophils, NOX2 is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species (ROS) in a process dependent on microtubules (X-ROS signaling).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat, By Heat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CYBB using anti-CYBB antibody (PA1667).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U937 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYBB antigen affinity purified polyclonal antibody (Catalog # PA1667) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CYBB at approximately 65 kDa. The expected band size for CYBB is at 65 kDa.
Click image to see more details
Figure 2. IHC analysis of CYBB using anti-CYBB antibody (PA1667).
CYBB was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CYBB Antibody (PA1667) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of CYBB using anti-CYBB antibody (PA1667).
CYBB was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CYBB Antibody (PA1667) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CYBB (Source: Uniprot.org, NCBI)
Gene Name
CYBB
Full Name
Cytochrome b-245 heavy chain
Weight
65.336kDa
Alternative Names
CGD; CGD91-phox; Cytochrome b(558) subunit beta; cytochrome b-245 heavy chain; cytochrome b-245, beta polypeptide; Cytochrome b558 subunit beta; EC 1.6.3; GP91-1; GP91PHOX; GP91-PHOX; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; NOX2chronic granulomatous disease; p22 phagocyte B-cytochrome; p91-PHOX; Superoxide-generating NADPH oxidase heavy chain subunit CYBB AMCBX2, CGD, GP91-1, GP91-PHOX, GP91PHOX, IMD34, NOX2, p91-PHOX cytochrome b-245 beta chain cytochrome b-245 heavy chain|CGD91-phox|NADPH oxidase 2|cytochrome b(558) subunit beta|cytochrome b-245 beta polypeptide|cytochrome b558 subunit beta|heme-binding membrane glycoprotein gp91phox|neutrophil cytochrome b 91 kDa polypeptide|p22 phagocyte B-cytochrome|superoxide-generating NADPH oxidase heavy chain subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYBB, check out the CYBB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYBB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-NOX2/gp91phox/CYBB Antibody (PA1667)
Hello CJ!
PA1667 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The Protective Roles of Estrogen Receptor β in Renal Calcium Oxalate Crystal Formation via Reducing the Liver Oxalate Biosynthesis and Renal Oxidative Stress-Mediated Cell Injury
Fetal e-cigarette exposure programs a neonatal brain hypoxic-ischemic sensitive phenotype via altering DNA methylation patterns and autophagy signaling pathway
Manuel R,Lima MdS,Dilly S,Daunay S,Abbe P,Pramil E,Solier S,Guillaumond F,Tubiana S-S,Escargueil A,Pêgas Henriques JA,Ferrand N,Erdelmeier I,Boucher J-L,Bertho G,Agranat I,Rocchi S,Sabbah M,Slama Schwok A.Distinction between 2′- and 3′-Phosphate Isomers of a Fluorescent NADPH Analogue Led to Strong Inhibition of Cancer Cells Migration.Antioxidants.2021; 10(5):723.https://doi.org/10.3390/antiox10050723
Species: Human,Mouse
PA1667 usage in article: APP:WB, SAMPLE:BREAST TISSUE, DILUTION:1:1000
Zhou H,Wang H,Shi N,Wu F.Potential Protective Effects of the Water-Soluble Chinese Propolis on Hypertension Induced by High-Salt Intake. Clin Transl Sci.2020 Sep;13(5):907-915.doi:10.1111/cts.12770.Epub 2020 Apr 8.PMID:32112504.
Species: Rat
PA1667 usage in article: APP:WB, SAMPLE: KIDNEY,HEART MUSCLE AND VASCULAR TISSUES, DILUTION:NA
Obligatory role for GPER in cardiovascular aging and disease^
Maternal inflammation activated ROS-p38 MAPK predisposes offspring to heart damages caused by isoproterenol via augmenting ROS generation
Graviola inhibits hypoxia-induced NADPH oxidase activity in prostate cancer cells reducing their proliferation and clonogenicity
Du Z, Yang Q, Liu L, Li S, Zhao J, Hu J, Liu C, Qian D, Gao C. Neuroscience. 2015 Feb 12;286:281-92. Doi: 10.1016/J.Neuroscience.2014.11.061. Epub 2014 Dec 8. Nadph Oxidase 2-Dependent Oxidative Stress, Mitochondrial Damage And Apoptosis In The Ve...
Du Z, Yang Q, Zhou T, Liu L, Li S, Chen S, Gao C. Mol Med Rep. 2014 Dec;10(6):2861-7. Doi: 10.3892/Mmr.2014.2653. Epub 2014 Oct 15. D?
Wang Y, Wang Y, Liu D, Wang W, Zhao H, Wang M, Yin H. Carbohydr Polym. 2015 Jul 10;125:135-45. Doi: 10.1016/J.Carbpol.2015.02.012. Epub 2015 Feb 19. Cordyceps Sinensis Polysaccharide Inhibits Pdgf-Bb-Induced Inflammation And Ros Production In Huma...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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3 Customer Q&As for Anti-NOX2/gp91phox/CYBB Antibody
Question
What is the recommended antigen retrieval method for PA1667?
Verified customer
Asked: 2019-05-14
Answer
The Anti-NOX2/Gp91phox/CYBB Antibody (PA1667) recommended antigen retrieval method; Heat-induced epitope retrieval: Immerse the slides in antigen retrieval buffer (citrate buffer, pH 6.0). Microwave the solution to boiling for three cycles of 5-10 min each. Cool the slides to room temperature. Wash the slides in PBS two times.
Boster Scientific Support
Answered: 2019-05-14
Question
We are currently using anti-NOX2/gp91phox/CYBB antibody PA1667 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?
R. Williams
Verified customer
Asked: 2014-12-19
Answer
The anti-NOX2/gp91phox/CYBB antibody (PA1667) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-12-19
Question
This is Chia-Yi from GeneTex. Hope you are doing well!
Verified Customer
Verified customer
Asked: 2013-05-20
Answer
Please see the following recommended antigen retrieval method for PA1667. Heat-induced epitope
Boster Scientific Support
Answered: 2013-05-20