Anti-NOX2/gp91phox/CYBB Antibody

CYBB/NOX2 antibody

Boster Bio Anti-NOX2/gp91phox/CYBB Antibody catalog # PA1667. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: PA1667
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-NOX2/gp91phox/CYBB Antibody

View all CYBB/NOX2 Antibodies

SKU/Catalog Number

PA1667

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NOX2/gp91phox/CYBB Antibody catalog # PA1667. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NOX2/gp91phox/CYBB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1667)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human NOX2, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1667 is reactive to CYBB in Human, Mouse, Rat

Applications

PA1667 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

65 kDa

Calculated molecular weight

65.336kDa

Background of CYBB/NOX2

NOX2 (NADPH OXIDASE 2), also called CYBB (CYTOCHROME b (-245), BETA SUBUNIT), p91-PHOX or GP91-1, is a human gene encoding a glycoprotein.NOX2 is an essential component of phagocytic NADPH-oxidase, a membrane-bound enzyme complex that generates large quantities of microbicidal superoxide and other oxidants upon activation. It is mapped on Xp11.4. NOX2 is a heterodimer composed of an alpha chain of relative molecular mass 23 kD and a beta chain of 76 to 82 kD. NOX2 assembled on DC phagosomes in a gp91-phox subunit-dependent manner, and that reactive oxygen species were produced in a more sustained manner in immature DC phagosomes than in macrophage phagosomes. As a major player in innate immune responses in neutrophils, NOX2 is also involved in adaptive immunity through its activity in DCs. In heart cells, physiologic stretch rapidly activates reduced-form NOX2 to produce reactive oxygen species (ROS) in a process dependent on microtubules (X-ROS signaling).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat, By Heat

Validation Images & Assay Conditions

Gene/Protein Information For CYBB (Source: Uniprot.org, NCBI)

Gene Name

CYBB

Full Name

Cytochrome b-245 heavy chain

Weight

65.336kDa

Alternative Names

CGD; CGD91-phox; Cytochrome b(558) subunit beta; cytochrome b-245 heavy chain; cytochrome b-245, beta polypeptide; Cytochrome b558 subunit beta; EC 1.6.3; GP91-1; GP91PHOX; GP91-PHOX; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; NOX2chronic granulomatous disease; p22 phagocyte B-cytochrome; p91-PHOX; Superoxide-generating NADPH oxidase heavy chain subunit CYBB AMCBX2, CGD, GP91-1, GP91-PHOX, GP91PHOX, IMD34, NOX2, p91-PHOX cytochrome b-245 beta chain cytochrome b-245 heavy chain|CGD91-phox|NADPH oxidase 2|cytochrome b(558) subunit beta|cytochrome b-245 beta polypeptide|cytochrome b558 subunit beta|heme-binding membrane glycoprotein gp91phox|neutrophil cytochrome b 91 kDa polypeptide|p22 phagocyte B-cytochrome|superoxide-generating NADPH oxidase heavy chain subunit

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CYBB, check out the CYBB Infographic

CYBB infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CYBB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1667 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The Protective Roles of Estrogen Receptor β in Renal Calcium Oxalate Crystal Formation via Reducing the Liver Oxalate Biosynthesis and Renal Oxidative Stress-Mediated Cell Injury

Fetal e-cigarette exposure programs a neonatal brain hypoxic-ischemic sensitive phenotype via altering DNA methylation patterns and autophagy signaling pathway

Manuel R,Lima MdS,Dilly S,Daunay S,Abbe P,Pramil E,Solier S,Guillaumond F,Tubiana S-S,Escargueil A,Pêgas Henriques JA,Ferrand N,Erdelmeier I,Boucher J-L,Bertho G,Agranat I,Rocchi S,Sabbah M,Slama Schwok A.Distinction between 2′- and 3′-Phosphate Isomers of a Fluorescent NADPH Analogue Led to Strong Inhibition of Cancer Cells Migration.Antioxidants.2021; 10(5):723.https://doi.org/10.3390/antiox10050723
Species: Human,Mouse
PA1667 usage in article: APP:WB, SAMPLE:BREAST TISSUE, DILUTION:1:1000

Zhou H,Wang H,Shi N,Wu F.Potential Protective Effects of the Water-Soluble Chinese Propolis on Hypertension Induced by High-Salt Intake. Clin Transl Sci.2020 Sep;13(5):907-915.doi:10.1111/cts.12770.Epub 2020 Apr 8.PMID:32112504.
Species: Rat
PA1667 usage in article: APP:WB, SAMPLE: KIDNEY,HEART MUSCLE AND VASCULAR TISSUES, DILUTION:NA

Obligatory role for GPER in cardiovascular aging and disease^

Maternal inflammation activated ROS-p38 MAPK predisposes offspring to heart damages caused by isoproterenol via augmenting ROS generation

Graviola inhibits hypoxia-induced NADPH oxidase activity in prostate cancer cells reducing their proliferation and clonogenicity

Du Z, Yang Q, Liu L, Li S, Zhao J, Hu J, Liu C, Qian D, Gao C. Neuroscience. 2015 Feb 12;286:281-92. Doi: 10.1016/J.Neuroscience.2014.11.061. Epub 2014 Dec 8. Nadph Oxidase 2-Dependent Oxidative Stress, Mitochondrial Damage And Apoptosis In The Ve...

Du Z, Yang Q, Zhou T, Liu L, Li S, Chen S, Gao C. Mol Med Rep. 2014 Dec;10(6):2861-7. Doi: 10.3892/Mmr.2014.2653. Epub 2014 Oct 15. D?

Wang Y, Wang Y, Liu D, Wang W, Zhao H, Wang M, Yin H. Carbohydr Polym. 2015 Jul 10;125:135-45. Doi: 10.1016/J.Carbpol.2015.02.012. Epub 2015 Feb 19. Cordyceps Sinensis Polysaccharide Inhibits Pdgf-Bb-Induced Inflammation And Ros Production In Huma...

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3 Customer Q&As for Anti-NOX2/gp91phox/CYBB Antibody

Question

What is the recommended antigen retrieval method for PA1667?

Verified customer

Asked: 2019-05-14

Answer

The Anti-NOX2/Gp91phox/CYBB Antibody (PA1667) recommended antigen retrieval method; Heat-induced epitope retrieval: Immerse the slides in antigen retrieval buffer (citrate buffer, pH 6.0). Microwave the solution to boiling for three cycles of 5-10 min each. Cool the slides to room temperature. Wash the slides in PBS two times.

Boster Scientific Support

Answered: 2019-05-14

Question

We are currently using anti-NOX2/gp91phox/CYBB antibody PA1667 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

R. Williams

Verified customer

Asked: 2014-12-19

Answer

The anti-NOX2/gp91phox/CYBB antibody (PA1667) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-12-19

Question

This is Chia-Yi from GeneTex. Hope you are doing well!

Verified Customer

Verified customer

Asked: 2013-05-20

Answer

Please see the following recommended antigen retrieval method for PA1667. Heat-induced epitope

Boster Scientific Support

Answered: 2013-05-20

Order DetailsPrice
PA1667

100μg

$370
PA1667-10ug

10μg sample (liquid)

$99
PA1667-Biotin

100 μg Biotin conjugated

$570
PA1667-Cy3

100 μg Cy3 conjugated

$570
PA1667-Dylight488

100 μg Dylight488 conjugated

$570
PA1667-Dylight550

100 μg Dylight550 conjugated

$570
PA1667-Dylight594

100 μg Dylight594 conjugated

$570
PA1667-FITC

100 μg FITC conjugated

$570
PA1667-HRP

100 μg HRP conjugated

$570
PA1667-APC

100 μg APC conjugated

$670
PA1667-PE

100 μg PE conjugated

$670
PA1667-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
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