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SKU:PA1930
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-NOXA1 Antibody
SKU/Catalog Number PA1930
Description Rabbit IgG polyclonal antibody for NADPH oxidase activator 1(NOXA1) detection. Tested with WB in Human.
Cite This Product Anti-NOXA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1930)
Replacement Item This antibody may replace the following items: sc-160597-R|sc-398873|sc-160596|sc-160598-R from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human NOXA1(176-195aa RQVPRGEVFRPHRWHLKHLE).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name NOXA1
Protein Name NADPH oxidase activator 1
Molecular Weight 50933 MW
Protein Function Functions as an activator of NOX1, a superoxide- producing NADPH oxidase. Functions in the production of reactive oxygen species (ROS) which participate in a variety of biological processes including host defense, hormone biosynthesis, oxygen sensing and signal transduction. May also activate CYBB/gp91phox and NOX3. .
Tissue Specificity Widely expressed. Detected in pancreas, liver, kidney, spleen, prostate, small intestine and colon. .
Sequence Similarities Belongs to the NCF2/NOXA1 family.
Subcellular Localization Cytoplasm. Cell membrane. Translocation to membranes depends on NOXO1 or NCF1 and maybe RAC1.
Uniprot ID Q86UR1
Alternative Names NADPH oxidase activator 1;NOX activator 1;Antigen NY-CO-31;NCF2-like protein;P67phox-like factor;p51-nox;NOXA1;P51NOX;
Research Areas |cardiovascular|hypoxia|associated proteins| signal transduction|protein trafficking|organelle proteins| cell biology|other antibodies|oxidative stress| metabolism|pathways and processes|redox metabolism|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for NADPH oxidase activator 1

NOXA1(NADPH oxidase activator 1), also called NOX ACTIVATOR 1 or p51-NOX, is an enzyme that in humans is encoded by the NOXA1 gene. Hartz(2007) mapped the NOXA1 gene to chromosome 9q34.3 based on an alignment of the NOXA1 sequence with the genomic sequence(build 36.1). Banfi et al.(2003) mapped the mouse Noxa1 gene to chromosome 2. Using yeast 2-hybrid assays, Takeya et al.(2003) showed that human p51-NOX interacted with constitutively active forms of RAC1 and RAC2. In vitro binding assays revealed that p51-NOX bound GTP-bound RAC1, but not GDP-bound RAC1. p51-NOX also bound p47-PHOX(NCF1) and p41-NOX(NOXO1), and trp436 within the SH3 domain of p51-NOX was required for these interactions. Human cell lines or COS-7 cells cotransfected with p51-NOX and p41-NOX and either gp91-PHOX(CYBB) or NOX1 produced superoxide. Cells individually transfected with NOX1, p41-NOX, or p51-NOX and cells transfected with only p41-NOX and p51-NOX showed no superoxide production.

Anti-NOXA1 Antibody Images

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Anti-NOXA1 Antibody
Anti-NOXA1 antibody, PA1930, Western blotting
Lane 1: U87 Cell Lysate
Lane 2: HELA Cell Lysate
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.