SKU PA1991
Size 100μg/vial
Reactivity Human
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications WB

Overview

Product Name Anti-NPM2 Antibody
SKU/Catalog Number PA1991
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Nucleoplasmin-2(NPM2) detection. Tested with WB in Human.
Cite This Product Anti-NPM2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1991)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human NPM2(25-43aa QERRTWTFRPQLEGKQSCR).
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

Anti-NPM2 antibody, PA1991, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: U87 Cell Lysate
Lane 3: A549 Cell Lysate
Lane 4: SMMC Cell Lysate

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q86SE8
Gene Name NPM2
Protein Name Nucleoplasmin-2
Alternative Names Nucleoplasmin-2;NPM2;
Subcellular Localization Nucleus . Found in the oocyte nucleus before nuclear membrane breakdown, after which it is redistributed to the cytoplasm. .
Molecular Weight 24152 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Core histones chaperone involved in chromatin reprogramming, specially during fertilization and early embryonic development. Probably involved in sperm DNA decondensation during fertilization. .
Research Areas Human

*You can search these to find other products in these research areas.
Background NPM2(Nucleophosmin/Nucleoplasmin Family Member 2), Burns et al.(2003) mapped the mouse Npm2 gene to chromosome 14. Burns et al.(2003) found that female Npm2-null mice showed defects in preimplantation embryo development, with abnormalities in oocyte and early embryonic nuclei.

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Polyclonal antibody for NUCLEOPLASMIN 2/NPM2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. NUCLEOPLASMIN 2/NPM2 information: Molecular Weight: 24152 MW; Subcellular Localization: Nucleus . Found in the oocyte nucleus before nuclear membrane breakdown, after which it is redistributed to the cytoplasm.
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In stock
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PA1991
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

Troubleshooting

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Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
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