Anti-NRBF-2 Antibody

NRBF2 antibody

Boster Bio Anti-NRBF-2 Antibody catalog # A09144. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A09144
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-NRBF-2 Antibody

View all NRBF2 Antibodies

SKU/Catalog Number

A09144

Size

100μl

Form

Liquid

Description

Boster Bio Anti-NRBF-2 Antibody catalog # A09144. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NRBF-2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09144)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

3D5B1

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human NRBF-2. at AA rangle: 110-190

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A09144 is reactive to NRBF2 in Human, Mouse, Rat

Applications

A09144 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

32.378kDa

Background of NRBF2

APC11 is also known as Anaphase promoting complex subunit 11, APC11, Cyclosome subunit 11, Hepatocellular carcinoma associated RING finger protein, and HSPC214. APC11 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC11 may function to recruit the E2 ubiquitin-conjugating enzymes to the complex. APC11 interacts with the cullin domain of ANAPC2 and also interacts with UBE2D2. APC11 shows both a cytoplasmic and nuclear localization. APC11 is expressed at high levels in skeletal muscle and heart; in moderate levels in brain, kidney, and liver; and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte. APC11 is a member of the RING-type zinc finger family and is auto-ubiquitinylated.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For NRBF2 (Source: Uniprot.org, NCBI)

Gene Name

NRBF2

Full Name

Nuclear receptor-binding factor 2

Weight

32.378kDa

Alternative Names

Comodulator of PPAR and RXR; COPR; COPR1; COPR2; DKFZp564C1664; FLJ30395; NRBF-2; nuclear receptor binding factor 2; nuclear receptor binding factor-2; nuclear receptor-binding factor 2 NRBF2 COPR, COPR1, COPR2, NRBF-2 nuclear receptor binding factor 2 nuclear receptor-binding factor 2|comodulator of PPAR and RXR 1|comodulator of PPAR and RXR 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NRBF2, check out the NRBF2 Infographic

NRBF2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NRBF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-NRBF-2 Antibody

Question

Can you help my question with product A09144, anti-NRBF-2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-01-02

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09144 anti-NRBF-2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-01-02

Question

Does A09144 anti-NRBF-2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-09-22

Answer

As indicated on the product datasheet, A09144 anti-NRBF-2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-09-22

Question

We are currently using anti-NRBF-2 antibody A09144 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2017-06-20

Answer

The anti-NRBF-2 antibody (A09144) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-06-20

Question

We want to test anti-NRBF-2 antibody A09144 on rat cervix carcinoma erythroleukemia for research purposes, then I may be interested in using anti-NRBF-2 antibody A09144 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

E. Kulkarni

Verified customer

Asked: 2015-11-11

Answer

The products we sell, including anti-NRBF-2 antibody A09144, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2015-11-11

Order DetailsPrice
A09144

100uL

$399

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