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SKU:PA1828
Clonality:Polyclonal
Application:IHC-P, ICC, WB
Price: $200.00
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Overview

Product Name Anti-Nrf2 Antibody
Description Rabbit IgG polyclonal antibody for Nuclear factor erythroid 2-related factor 2(NFE2L2) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
Cite This Product Anti-Nrf2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1828)
Replacement Item This antibody may replace the following items: sc-1225|sc-7148|sc-7272|sc-56052|sc-56051|sc-65497|sc-70498|sc-56046|sc-65496|sc-70497|sc-56053|sc-56050|sc-136219|sc-1224|sc-1225|sc-1226|sc-136219|sc-166589|sc-22139|sc-22140|sc-22171-R|sc-271028|sc-271759|sc-373730|sc-56046|sc-56050|sc-56051|sc-56052|sc-56053|sc-6138|sc-65496|sc-65497|sc-70497|sc-70498|sc-7148|sc-7272|sc-98785 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Chicken

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Nrf2(580-596aa EYSLQQTRDGNVFLVPK), identical to the related mouse and rat sequences.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name NFE2L2
Protein Name Nuclear factor erythroid 2-related factor 2
Molecular Weight 67827 MW
Protein Function Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. .
Tissue Specificity Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.
Sequence Similarities Belongs to the bZIP family. CNC subfamily.
Subcellular Localization Cytoplasm, cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
Uniprot ID Q16236
Alternative Names Nuclear factor erythroid 2-related factor 2;NF-E2-related factor 2;NFE2-related factor 2;HEBP1;Nuclear factor, erythroid derived 2, like 2;NFE2L2;NRF2;
Research Areas NFE2L2|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Nuclear factor erythroid 2-related factor 2

NFE2L2(nuclear factor(erythroid-derived 2)-like 2) also known as NRF2 or NFE2-RELATED TRANSCRIPTION FACTOR 2, is a transcription factor that in humans is encoded by theNFE2L2 gene. NFE2, NFE2L1, and NFE2L2 comprise a family of human genes encoding basic leucine zipper(bZIP) transcription factors. NFE2L2 induces the expression of various genes including those that encode for several antioxidant enzymes, and it may play a physiological role in the regulation of oxidative stress. The NFE2L2 gene is located on 2q31.2. The identification of somatic mutations that disrupt the NRF2-KEAP1 interaction to stabilize NRF2 and increase the constitutive transcription of NRF2 target genes indicated that enhanced reactive oxygen species(ROS) detoxification and additional NRF2 functions may in fact be tumorigenic. Oncogene-directed increased expression of Nrf2 is a mechanism for the activation of the Nrf2 antioxidant program evident in primary cells and tissues of mice expressing KRas(G12D) and BRaf(V619E), and in human pancreatic cancer.

Anti-Nrf2 Antibody Images

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Anti-Nrf2 Antibody
Anti-Nrf2 antibody, PA1828, Western blotting
All lanes: Anti Nrf2 (PA1828) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: SMMC Whole Cell Lysate at 40ug
Lane 5: MM231Whole Cell Lysate at 40ug
Predicted bind size: 68KD
Observed bind size: 68KD
Anti-Nrf2 Antibody
Anti-Nrf2 antibody, PA1828, IHC(P)
IHC(P): Rat Kidney Tissue
Anti-Nrf2 Antibody
Anti-Nrf2 antibody, PA1828, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Anti-Nrf2 Antibody
Anti-Nrf2 antibody, PA1828, ICC
ICC: A549 Cell
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Publications

Wang Qm, Du Jl, Duan Zj, Guo Sb, Sun Xy, Liu Z. World J Gastroenterol. 2013 May 21;19(19):2921-34. Doi: 10.3748/Wjg.V19.I19.2921. Inhibiting Heme Oxygenase-1 Attenuates Rat Liver Fibrosis By Removing Iron Accumulation.
Li Y, Xie K, Chen H, Wang G, Yu Y. J Surg Res. 2015 Jun 1;196(1):136-48. Doi: 10.1016/J.Jss.2015.02.042. Epub 2015 Feb 27. Hydrogen Gas Inhibits High-Mobility Group Box 1 Release In Septic Mice By Upregulation Of Heme Oxygenase 1.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to nrf2 antibody, nfe2l2 antibody, nrf 2 antibody