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SKU:PA1061
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-NSE Antibody
Description Rabbit IgG polyclonal antibody for Gamma-enolase(ENO2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-NSE Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1061)
Replacement Item This antibody may replace the following items: sc-15343|sc-21737|sc-21738|sc-271384|sc-271792|sc-31857|sc-31858|sc-31859|sc-31860|sc-376375|sc-390163|sc-51880|sc-56384|sc-71046|sc-71047|sc-7455 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human NSE(420-434aa EARFAGHNFRNPSVL), identical to the related mouse and rat sequences.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name ENO2
Protein Name Gamma-enolase
Molecular Weight 47269 MW
Protein Function Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity). .
Tissue Specificity The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
Sequence Similarities Belongs to the enolase family.
Subcellular Localization Cytoplasm . Cell membrane . Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. .
Uniprot ID P09104
Alternative Names Gamma-enolase;4.2.1.11;2-phospho-D-glycerate hydro-lyase;Enolase 2;Neural enolase;Neuron-specific enolase;NSE;ENO2;
Research Areas |signal transduction|metabolism|energy metabolism| epigenetics and nuclear signaling|transcription|other factors| cancer|tumor biomarkers|enzymes|neuron specific enolase|cancer metabolism|metabolic signaling pathway|metabolism of carbohydrates| metabolism|pathways and processes|metabolic signaling pathways|carbohydrate metabolism|energy transfer pathways|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Gamma-enolase

NSE(neuron specific enolase), also known as Enolase 2(ENO2), is found in elevated concentrations in plasma in certain neoplasias. The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. ENO2 gene contains 12 exons distributed over 9,213 nucleotides. Human neurone-specific enolase is mapped to chromosome 12p13.

Anti-NSE Antibody Images

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Anti-NSE Antibody
Anti-NSE antibody, PA1061, Western blotting
WB: Rat Brain Tissue Lysate
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Publications

Li, Y., Guan, Y., Wang, Y., Yu, C., Zhai, F., & Guan, L. (2017). Neuroprotective effect of the Ginsenoside Rg1 on cerebral ischemic injury in vivo and in vitro is mediated by PPARγ-regulated anti-oxidative and anti-inflammatory pathways. Evidence-Based Complementary and Alternative Medicine. Advance online publication.
Zhou Sn, Li Cs, Liu Lq, Li Y, Wang Xf, Shen L. Histol Histopathol. 2011 May;26(5):571-9. Increased Expression Of Annexin A7 In Temporal Lobe Tissue Of Patients With Refractory Epilepsy.
He Y, Zeng Sy, Zhou Sw, Qian Gs, Peng K, Mo Zx, Zhou Jy. Fitoterapia. 2014 Oct;98:166-73. Doi: 10.1016/J.Fitote.2014.08.002. Epub 2014 Aug 7. Effects Of Rhynchophylline On Glun1 And Glun2B Expressions In Primary Cultured Hippocampal Neurons.
Huang H, Jiang L, Li S, Deng J, Li Y, Yao J, Li B, Zheng J. Biomicrofluidics. 2014 Feb 19;8(1):014108. Doi: 10.1063/1.4864235. Ecollection 2014. Using Microfluidic Chip To Form Brain-Derived Neurotrophic Factor Concentration Gradient For Studying ...
Wan J, Chai H, Yu Z, Ge W, Kang N, Xia W, Che Y. J Exp Clin Cancer Res. 2011 Aug 15;30:77. Doi: 10.1186/1756-9966-30-77. Hif-1?? Effects On Angiogenic Potential In Human Small Cell Lung Carcinoma.
Li K, Zheng S, Xiao X, Wang Q, Zhou Y, Chen L. J Pediatr Surg. 2007 Aug;42(8):1433-8. The Structural Characteristics And Expression Of Neuropeptides In The Esophagus Of Patients With Congenital Esophageal Atresia And Tracheoesophageal Fistula.
Zhang M, Chai Y, Liu T, Xu N, Yang C. Exp Ther Med. 2015 Apr;9(4):1141-1148. Epub 2015 Feb 2. Synergistic Effects Of Buyang Huanwu Decoction And Embryonic Neural Stem Cell Transplantation On The Recovery Of Neurological Function In A Rat Model Of ...
Du Y, Zou Y, Yu W, Shi R, Zhang M, Yang W, Duan J, Deng Y, Wang X, L?? Y. Brain Res. 2013 May 6;1509:79-85. Doi: 10.1016/J.Brainres.2013.03.005. Epub 2013 Mar 18. Expression Pattern Of Sorting Nexin 25 In Temporal Lobe Epilepsy: A Study On Patients...
Hu Wx, Xiang Q, Wen Z, He D, Wu Xm, Hu Gz. Mol Med Rep. 2014 Jun;9(6):2573-81. Doi: 10.3892/Mmr.2014.2105. Epub 2014 Apr 2. Neuroprotective Effect Of Atractylodes Macrocephalaon Polysaccharides In Vitro On Neuronal Apoptosis Induced By Hypoxia.
Wu J, Yang J, Liu Q, Wu S, Ma H, Cai Y. Biol Trace Elem Res. 2013 Apr;152(1):125-34. Doi: 10.1007/S12011-013-9601-3. Epub 2013 Jan 23. Lanthanum Induced Primary Neuronal Apoptosis Through Mitochondrial Dysfunction Modulated By Ca??
Zhang Z, Zhou X, Zhou X, Xu X, Liao M, Yan L, Lv R, Luo H. Neural Regen Res. 2012 May 5;7(13):971-7. Doi: 10.3969/J.Issn.1673-5374.2012.13.002. Methyl 3,4-Dihydroxybenzoate Promotes Neurite Outgrowth Of Cortical Neurons Cultured In Vitro.
Shao Jl, Wan Xh, Chen Y, Bi C, Chen Hm, Zhong Y, Heng Xh, Qian Jq. J Mol Neurosci. 2011 Mar;43(3):453-60. Doi: 10.1007/S12031-010-9464-4. Epub 2010 Oct 22. H2S Protects Hippocampal Neurons From Anoxia-Reoxygenation Through Camp-Mediated Pi3K/Akt/P...
Liu Q, Shen Y, Chen J, Gao X, Feng C, Wang L, Zhang Q, Jiang X. Pharm Res. 2012 Feb;29(2):546-58. Doi: 10.1007/S11095-011-0641-0. Epub 2011 Dec 14. Nose-To-Brain Transport Pathways Of Wheat Germ Agglutinin Conjugated Peg-Pla Nanoparticles.
Xiao F, Lin Lf, Cheng X, Gao Q, Luo Hm. Mol Med Rep. 2012 Mar;5(3):619-24. Doi: 10.3892/Mmr.2011.692. Epub 2011 Dec 1. Nogo-66 Receptor Activation Inhibits Neurite Outgrowth And Increases ??-Amyloid Protein Secretion Of Cortical Neurons.
Sun C, Shao J, Su L, Zhao J, Bi J, Yang S, Zhang S, Gao J, Miao J. Cell Transplant. 2013;22(6):961-75. Doi: 10.3727/096368912X657413. Epub 2012 Oct 1. Cholinergic Neuron-Like Cells Derived From Bone Marrow Stromal Cells Induced By Tricyclodecane-9...
Zhang X, Cai Z, Wang G, Wang H, Liu Z, Guo X, Yang C, Wang X, Wang H, Shu C, Xiao L. Behav Brain Res. 2013 Apr 15;243:165-70. Doi: 10.1016/J.Bbr.2013.01.001. Epub 2013 Jan 11. F-Actin May Play An Important Role In Il-1??-Stimulated Hippocampal Neur...
Liu X, Teng Z, Cui C, Wang R, Liu M, Zhang Y. J Mol Neurosci. 2014 Jan;52(1):124-30. Doi: 10.1007/S12031-013-0216-0. Epub 2014 Jan 10. Amyloid Beta-Derived Diffusible Ligands (Addls) Induce Abnormal Expression Of Insulin Receptors In Rat Hippocamp...
Cai Z, Zhang X, Wang G, Wang H, Liu Z, Guo X, Yang C, Wang X, Wang H, Shu C, Xiao L. Neuro Endocrinol Lett. 2014;35(1):13-9. Bdnf Attenuates Il-1??-Induced F-Actin Remodeling By Inhibiting Nf-??b Signaling In Hippocampal Neurons.
Pei H, Su X, Zhao L, Li H, Guo Y, Zhang M, Xin H. Int J Mol Sci. 2012;13(3):2551-62. Doi: 10.3390/Ijms13032551. Epub 2012 Feb 23. Primary Study For The Therapeutic Dose And Time Window Of Picroside Ii In Treating Cerebral Ischemic Injury In Rats.
Wu Y, Chen W, Guo M, He Q, Hu Y. Int J Clin Exp Pathol. 2014 Jul 15;7(8):4827-36. Ecollection 2014. Effects Of Transforming Growth Factor-??2 On Myocilin Expression And Secretion In Human Primary Cultured Trabecular Meshwork Cells.
Li F, Miao Zn, Xu Yy, Zheng Sy, Qin Md, Gu Yz, Zhang Xg. Mol Med Rep. 2012 Sep;6(3):625-30. Doi: 10.3892/Mmr.2012.968. Epub 2012 Jun 26. Transplantation Of Human Amniotic Mesenchymal Stem Cells In The Treatment Of Focal Cerebral Ischemia.
Liu Q, Cheng G, Wang Z, Zhan S, Xiong B, Zhao X. In Vitro Cell Dev Biol Anim. 2015 Mar;51(3):319-27. Doi: 10.1007/S11626-015-9875-1. Epub 2015 Mar 14. Bone Marrow-Derived Mesenchymal Stem Cells Differentiate Into Nerve-Like Cells In Vitro After Tr...
Lu Y, Yuan X, Ou Y, Cai Y, Wang S, Sun Q, Zhang W. Neural Regen Res. 2012 Jun 5;7(16):1205-12. Doi: 10.3969/J.Issn.1673-5374.2012.16.001. Autophagy And Apoptosis During Adult Adipose-Derived Stromal Cells Differentiation Into Neuron-Like Cells In ...
Wu Y, Ge C, Zeng W, Zhang C. Stem Cells Dev. 2010 Feb;19(2):195-202. Doi: 10.1089/Scd.2008.0383. Induced Multilineage Differentiation Of Chicken Embryonic Germ Cells Via Embryoid Body Formation.
Meng Xt, Chen D, Dong Zy, Liu Jm. Cell Biol Int. 2007 Jul;31(7):691-8. Epub 2007 Jan 19. Enhanced Neural Differentiation Of Neural Stem Cells And Neurite Growth By Amniotic Epithelial Cell Co-Culture.
Xu X, Qian H, Zhu W, Zhang X, Yan Y, Wang M, Xu W. J Mol Med (Berl). 2010 Nov;88(11):1181-90. Doi: 10.1007/S00109-010-0659-5. Epub 2010 Aug 10. Isolation Of Cancer Stem Cells From Transformed Human Mesenchymal Stem Cell Line F6.
Jiang R, Xu W, Zhu W, Chen M, Qian H, Qiao C, Yang H, Wang X, Chen Y. Oncol Rep. 2006 Nov;16(5):1021-8. Histological Type Of Oncogenity And Expression Of Cell Cycle Genes In Tumor Cells From Human Mesenchymal Stem Cells.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to neuron specific enolase antibody, neuron-specific enolase antibody, nse antibody