|Sample Size:||30ug for $99, contact us for details|
Data & Images
|Product Name||Anti-P GlycoProtein Antibody|
|Description||Rabbit IgG polyclonal antibody for Multidrug resistance protein 1(ABCB1) detection. Tested with IHC-P, IHC-F in Human;Mouse;Rat.|
|Cite This Product||Anti-P GlycoProtein Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1034)|
|Replacement Item||This antibody may replace the following items: sc-13131|sc-1517|sc-1517-R|sc-55510|sc-71557|sc-73354|sc-8313 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human P Glycoprotein(621-650aa IYFKLVTMQTAGNEVELENAADESKSEIDA), different from the related rat sequence by twelve amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Multidrug resistance protein 1|
|Molecular Weight||141479 MW|
|Protein Function||Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.|
|Tissue Specificity||Expressed in liver, kidney, small intestine and brain.|
|Sequence Similarities||Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.|
|Subcellular Localization||Cell membrane ; Multi-pass membrane protein .|
|Alternative Names||Multidrug resistance protein 1;188.8.131.52;ATP-binding cassette sub-family B member 1;P-glycoprotein 1;CD243;ABCB1;MDR1, PGY1;|
|Research Areas|||signal transduction|metabolism|mitochondrial| metabolism|pathways and processes|mitochondrial metabolism|mitochondrial markers||
Background for Multidrug resistance protein 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-P GlycoProtein Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Mouse, Rat, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-P GlycoProtein Antibody Images
Click the images to enlarge.
IHC(F): Mouse Intestine Tissue
IHC(F): Rat Kidney Tissue
IHC(P): Human Lung Cancer Tissue
IHC(P): Mouse Kidney Tissue
IHC(P): Rat Kidney Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,