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SKU:MA1060
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:F4
Application:IHC-P, IHC-F, ICC, WB
Price: $200.00
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Overview

Product Name Anti-P-GlycoProtein(MDR) Antibody (Monoclonal, F4)
SKU/Catalog Number MA1060
Description Mouse IgG monoclonal antibody for P-Glycoprotein (MDR), ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1 ) detection. Tested with WB, IHC-P, IHC-F, ICC in Human. No cross reactivity with other proteins.
Cite This Product Anti-P-GlycoProtein(MDR) Antibody (Monoclonal, F4) F4 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1060)
Replacement Item This antibody may replace the following items: sc-13131|sc-1517|sc-1517-R|sc-55510|sc-71557|sc-73354|sc-8313 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human
Application IHC-P, IHC-F, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Mixture of human and hamster drug-resistant whole cells and crude plasma membranes.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number F4
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name ABCB4
Protein Name Multidrug resistance protein 1
Molecular Weight 140647 MW
Protein Function Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. .
Tissue Specificity ABCB1: Expressed in liver, kidney, small intestineand brain.
Sequence Similarities Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
Subcellular Localization Cell membrane; Multi-pass membrane protein.
Uniprot ID P21447
Alternative Names Multidrug resistance protein 1A;3.6.3.44;ATP-binding cassette sub-family B member 1A;MDR1A;Multidrug resistance protein 3;P-glycoprotein 3;Abcb1a;Abcb4, Mdr1a, Mdr3, Pgy-3, Pgy3;
Research Areas signal transduction|metabolism|mitochondrial|pathways and processes|mitochondrial metabolism|mitochondrial markers|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Multidrug resistance protein 1

P-Glycoprotein,, also known as Multidrug Resistance 1(MDR1), is one of the ATP-binding cassette transporters family. P-glycoprotein-1 is involved in the transport of 3 of these protease inhibitors in vitro. MDR1 gene is mapped to the 7q21.1 by in situ hybridization. The MDR1 gene product, P-glycoprotein, mediates the transport of the cardiac glycoside, digoxin.
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Publications

Gao S, Liu Q, Wang X, Lin B, Zhang S. Med Oncol. 2010 Sep;27(3):960-7. Doi: 10.1007/S12032-009-9317-6. Epub 2009 Sep 23. Effects Of Lewis Y Antigen On The Gene Expression Of Multiple Drug Resistance-Associated Proteins In Human Ovarian Cancer Rmg-...
Wang X, Deng R, Lu Y, Xu Q, Yan M, Ye D, Chen W. Basic Clin Pharmacol Toxicol. 2013 Jan;112(1):25-33. Doi: 10.1111/J.1742-7843.2012.00921.X. Epub 2012 Aug 22. Gambogic Acid As A Non-Competitive Inhibitor Of Atp-Binding Cassette Transporter B1 Reve...

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to abcb1 antibody, mdr1 antibody, p gp antibody