Anti-p16 CDKN2A Monoclonal Antibody

Boster Bio Anti-p16 CDKN2A Monoclonal Antibody catalog # A00016-2. Tested in IHC, WB applications. This antibody reacts with Human, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: A00016-2
Size: 100μl
Reactive Species: Human, Rat
Host: Mouse
Application: IHC, WB

Product Name

Anti-p16 CDKN2A Monoclonal Antibody

See all CDKN2A products

SKU/Catalog Number

A00016-2

Size

100μl

Form

Liquid

Description

Boster Bio Anti-p16 CDKN2A Monoclonal Antibody catalog # A00016-2. Tested in IHC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-p16 CDKN2A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00016-2)

Host

Mouse

Contents

Ascitic fluid containing 0.03% sodium azide, 0.5% BSA, 50% glycerol.

Clonality

Monoclonal

Clone Number

2D9A12

Isotype

IgG2b

Immunogen

Purified recombinant fragment of p16 expressed in E. Coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00016-2 is reactive to CDKN2A in Human, Rat

Applications

A00016-2 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000
IHC, 1:200-1:1000

Validation Images & Assay Conditions

Gene/Protein Information For CDKN2A (Source: Uniprot.Org, NCBI)

Uniprot ID

Q8N726

Gene ID

1029

Gene Name

CDKN2A

Full Name

Tumor suppressor ARF

Weight

13.903kDa

Alternative Names

alternative reading frame; ARF; ARF-INK4a; CDK4I; CDKN2A; Cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A (p16, inhibits CDK4); cyclin-dependent kinase inhibitor 2A; cyclin-dependent kinase inhibitor 2A, isoform 3; cyclin-dependent kinase inhibitor 2A, isoforms 1/2; cyclin-dependent kinase inhibitor protein; INK4; INK4a; Ink4a/Arf; INK4a-ARF; mitochondrial smARF'; MTS1; p14; p14ARF; P16; p16(INK4a); p16-INK4; P16INK4A; p16-INK4a; P19; p19<ARF>; p19ARF; Pctr1 CDKN2A ARF, CDK4I, CDKN2, CMM2, INK4, INK4A, MLM, MTS-1, MTS1, P14, P14ARF, P16, P16-INK4A, P16INK4, P16INK4A, P19, P19ARF, TP16 cyclin dependent kinase inhibitor 2A cyclin-dependent kinase inhibitor 2A|CDK4 inhibitor p16-INK4|alternative reading frame|cell cycle negative regulator beta|cyclin-dependent kinase 4 inhibitor A|cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)|multiple tumor suppressor 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CDKN2A, check out the CDKN2A Infographic

CDKN2A infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CDKN2A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00016-2 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Expression of p16 protein in infantile hemangioma

Construction of pETNF-P16 plasmid and its expression properties in EC9706 cell line induced by X-ray irradiation

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12 Customer Q&As for Anti-p16 CDKN2A Monoclonal Antibody

Question

Is a blocking peptide available for product anti-p16 Monoclonal antibody (A00016-2)?

Verified Customer

Verified customer

Asked: 2020-02-12

Answer

We do provide the blocking peptide for product anti-p16 Monoclonal antibody (A00016-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-02-12

Question

I see that the anti-p16 Monoclonal antibody A00016-2 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-19

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-19

Question

Will anti-p16 Monoclonal antibody A00016-2 work for WB with adenohypophysis?

Verified Customer

Verified customer

Asked: 2019-08-14

Answer

According to the expression profile of adenohypophysis, CDKN2A is highly expressed in adenohypophysis. So, it is likely that anti-p16 Monoclonal antibody A00016-2 will work for WB with adenohypophysis.

Boster Scientific Support

Answered: 2019-08-14

Question

I was wanting to use your anti-p16 Monoclonal antibody for WB for human adenohypophysis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human adenohypophysis identification?

Verified Customer

Verified customer

Asked: 2019-05-16

Answer

It shows on the product datasheet, A00016-2 anti-p16 Monoclonal antibody has been validated for IHC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human adenohypophysis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-16

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adenohypophysis using anti-p16 Monoclonal antibody A00016-2. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-05-14

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-14

Question

Do you have a BSA free version of anti-p16 Monoclonal antibody A00016-2 available?

Verified Customer

Verified customer

Asked: 2018-12-20

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-p16 Monoclonal antibody A00016-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-12-20

Question

Would A00016-2 anti-p16 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

V. Wu

Verified customer

Asked: 2018-08-08

Answer

As indicated on the product datasheet, A00016-2 anti-p16 Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-08-08

Question

We are currently using anti-p16 Monoclonal antibody A00016-2 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2018-05-02

Answer

The anti-p16 Monoclonal antibody (A00016-2) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-05-02

Question

you antibody to test anti-p16 Monoclonal antibody A00016-2 on human adenohypophysis for research purposes, then I may be interested in using anti-p16 Monoclonal antibody A00016-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

M. Banerjee

Verified customer

Asked: 2018-01-31

Answer

The products we sell, including anti-p16 Monoclonal antibody A00016-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-01-31

Question

Here is the WB image, lot number and protocol we used for adenohypophysis using anti-p16 Monoclonal antibody A00016-2. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-01-26

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-01-26

Question

Is this A00016-2 anti-p16 Monoclonal antibody reactive to the isotypes of CDKN2A?

R. Jones

Verified customer

Asked: 2014-04-18

Answer

The immunogen of A00016-2 anti-p16 Monoclonal antibody is Purified recombinant fragment of p16 expressed in E. Coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-04-18

Question

Can you help my question with product A00016-2, anti-p16 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

B. Rodriguez

Verified customer

Asked: 2013-09-17

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00016-2 anti-p16 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-09-17

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A00016-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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