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SKU:MA1074
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:DCS-50
Application:IHC-F, ICC, WB
Price: $200.00
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Overview

Product Name Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50)
SKU/Catalog Number MA1074
Description Mouse IgG monoclonal antibody for p16INK4a/CDKN2, cyclin-dependent kinase inhibitor 2A (CDKN2A) detection. Tested with WB, IHC-F, ICC in Human. No cross reactivity with other proteins.
Cite This Product Anti-p16INK4a/CDKN2 Antibody (Monoclonal, DCS-50) DCS-50 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1074)
Replacement Item This antibody may replace the following items: sc-161477|sc-161478|sc-164595|sc-164596|sc-164597|sc-246240|sc-246241|sc-28260|sc-30545|sc-30547|sc-323702|sc-323703|sc-323704|sc-377412|sc-53392|sc-53639|sc-53640|sc-71797|sc-73434|sc-81841|sc-8340|sc-8613 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human
Application IHC-F, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Recombinant human p16 protein.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number DCS-50
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Cdkn2a
Protein Name Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3
Molecular Weight 17366 MW
Protein Function Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein (By similarity). .
Tissue Specificity Expressed in spleen, liver and lung. Not detected in kidney, colon, stomach or brain. .
Sequence Similarities Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.
Subcellular Localization Cytoplasm . Nucleus .
Uniprot ID Q9R0Z3
Alternative Names Cyclin-dependent kinase inhibitor 2A ;Cyclin-dependent kinase 4 inhibitor A;CDK4I;p16-INK4a;p16;p16-INK4;Cdkn2a ;P16ink4a ;
Research Areas |cell biology|cell cycle|cell cycle inhibitors|ink4| epigenetics and nuclear signaling|transcription|cancer susceptibility|tumor suppressors| cancer|oncoproteins/suppressors|p53 pathway|ink4a/arf|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3

p16(INK4A), also known as cyclin-dependent kinase inhibitor 2A(CDKN2A), or multiple tumor suppressor 1(MTS1). The p16 gene(CDKN2A) was mapped to 9p21. The p16 gene encodes a negative regulator of the cell cycle. CDKN2 plays an important role during tumorigenesis or tumor progression in a significant proportion of pancreatic adenocarcinomas. Germ-line mutations in the CDKN2A tumor-suppressor gene have been linked to the development of melanoma in some families with inherited melanoma.
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FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to p19 antibody, arf antibody, cdkn2a antibody