|Sample Size:||30ug for $99, contact us for details|
Data & Images
|Product Name||Anti-P180 Lamellar Body Protein Antibody|
|Description||Rabbit IgG polyclonal antibody for ATP-binding cassette sub-family A member 3(ABCA3) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-P180 Lamellar Body Protein Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2259)|
|Replacement Item||This antibody may replace the following items: sc-48444|sc-134560|sc-48442|sc-48441 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human P180 Lamellar Body Protein(271-288aa FTYTALTIARAVVQEKER), different from the related mouse sequence by three amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||ATP-binding cassette sub-family A member 3|
|Molecular Weight||191362 MW|
|Protein Function||Plays an important role in the formation of pulmonary surfactant, probably by transporting lipids such as cholesterol.|
|Tissue Specificity||Highly expressed in lung, followed by brain, pancreas, skeletal muscle and heart. Weakly expressed in placenta, kidney and liver. Also expressed in medullary thyroid carcinoma cells (MTC) and in C-cell carcinoma.|
|Sequence Similarities||Belongs to the ABC transporter superfamily. ABCA family.|
|Subcellular Localization||Membrane ; Multi-pass membrane protein .|
|Alternative Names||ATP-binding cassette sub-family A member 3;ABC-C transporter;ATP-binding cassette transporter 3;ATP-binding cassette 3;ABCA3;ABC3;|
|Research Areas|||signal transduction|metabolism|lipid metabolism| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|cholesterol metabolism||
Background for ATP-binding cassette sub-family A member 3
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-P180 Lamellar Body Protein Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-P180 Lamellar Body Protein Antibody Images
Click the images to enlarge.
All lanes: Anti-ABCA3(PA2259) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 169KD
Observed bind size: 191KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,