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SKU:PA2259
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-P180 Lamellar Body Protein Antibody
Description Rabbit IgG polyclonal antibody for ATP-binding cassette sub-family A member 3(ABCA3) detection. Tested with WB in Human;Mouse;Rat.
Cite This Product Anti-P180 Lamellar Body Protein Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2259)
Replacement Item This antibody may replace the following items: sc-48444|sc-134560|sc-48442|sc-48441 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human P180 Lamellar Body Protein(271-288aa FTYTALTIARAVVQEKER), different from the related mouse sequence by three amino acids.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name ABCA3
Protein Name ATP-binding cassette sub-family A member 3
Molecular Weight 191362 MW
Protein Function Plays an important role in the formation of pulmonary surfactant, probably by transporting lipids such as cholesterol.
Tissue Specificity Highly expressed in lung, followed by brain, pancreas, skeletal muscle and heart. Weakly expressed in placenta, kidney and liver. Also expressed in medullary thyroid carcinoma cells (MTC) and in C-cell carcinoma.
Sequence Similarities Belongs to the ABC transporter superfamily. ABCA family.
Subcellular Localization Membrane ; Multi-pass membrane protein .
Uniprot ID Q99758
Alternative Names ATP-binding cassette sub-family A member 3;ABC-C transporter;ATP-binding cassette transporter 3;ATP-binding cassette 3;ABCA3;ABC3;
Research Areas |signal transduction|metabolism|lipid metabolism| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|cholesterol metabolism|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for ATP-binding cassette sub-family A member 3

ATP-binding cassette sub-family A member 3 is a protein that in humans is encoded by the ABCA3 gene. This protein is a member of the ABC1 subfamily. It is mapped to 16p13.3. ABCA3 is homologous to C. elegans protein ced7. Ced7 functions to translocate molecules that mediate homotypic adhesion between the cell surfaces of the dying and engulfing cells, and ABCA3 may be functionally similar to it. This gene is associated with Surfactant metabolism dysfunction type 3, and it plays an important role in the formation of pulmonary surfactant, probably by transporting lipids such as cholesterol. What's more, ABCA3 is also critical for the proper formation of lamellar bodies.

Anti-P180 Lamellar Body Protein Antibody Images

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Anti-P180 Lamellar Body Protein Antibody
Anti-P180 Lamellar Body Protein antibody, PA2259, All Western blotting
All lanes: Anti-ABCA3(PA2259) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 169KD
Observed bind size: 191KD
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is abca3 antibody