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SKU:MA1075
Clonality:Monoclonal
Clone Number:DCS-100
Application:IHC-P, ICC, WB
Price: $200.00
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Overview

Product Name Anti-p19INK4d Antibody (Monoclonal, DCS-100)
Description Mouse IgG monoclonal antibody for p19INK4d, cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) (CDKN2D) detection. Tested with WB, IHC-P, ICC in Human. No cross reactivity with other proteins.
Cite This Product Anti-p19INK4d Antibody (Monoclonal, DCS-100) DCS-100 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1075)
Replacement Item This antibody may replace the following items: sc-1063|sc-1063-G|sc-1076-G|sc-56334|sc-65594|sc-71807|sc-71808|sc-71809|sc-71810 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human
Application IHC-P, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Recombinant human p19INK4d.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number DCS-100
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Cdkn2d
Protein Name Cyclin-dependent kinase 4 inhibitor D
Molecular Weight 17809 MW
Protein Function Interacts strongly with CDK4 and CDK6 and inhibits them. .
Sequence Similarities Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.
Subcellular Localization Nucleus . Cytoplasm .
Uniprot ID Q60773
Alternative Names Cyclin-dependent kinase 4 inhibitor D;p19-INK4d;Cdkn2d;
Research Areas |cell biology|cell cycle|cell cycle inhibitors|ink4| epigenetics and nuclear signaling| cancer|oncoproteins/suppressors|tumor suppressors|ink4a/arf|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Cyclin-dependent kinase 4 inhibitor D

Cyclins are important in regulating the cell cycle through their formation of enzymatic complexes with various cyclin-dependent kinases. P19(INK4d) also kowns as cyclin-dependent kinase inhibitor 2D, is one of the novel members of the mouse INK4 gene family. Okuda et al.(1995) described the cloning of the human INK4d gene(CDKN2D). The predicted 166-amino acid protein is 86% identical to the mouse protein and about 45% identical to other human INK4 family members.
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FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is p19 antibody