Anti-p21 CDKN1A Rabbit Monoclonal Antibody

p21/CIP1/CDKN1A antibody

Boster Bio Anti-p21 CDKN1A Rabbit Monoclonal Antibody catalog # M00145-3. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human. Cited in 4 publication(s).

Product Info Summary

SKU: M00145-3
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IP, IF, IHC, ICC, WB

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Product Name

Anti-p21 CDKN1A Rabbit Monoclonal Antibody

View all p21/CIP1/CDKN1A Antibodies

SKU/Catalog Number

M00145-3

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-p21 CDKN1A Rabbit Monoclonal Antibody catalog # M00145-3. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-p21 CDKN1A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00145-3)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ACG-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human p21

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00145-3 is reactive to CDKN1A in Human

Applications

M00145-3 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

95 kDa

Calculated molecular weight

18.119kDa

Background of p21/CIP1/CDKN1A

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For CDKN1A (Source: Uniprot.org, NCBI)

Gene Name

CDKN1A

Full Name

Cyclin-dependent kinase inhibitor 1

Weight

18.119kDa

Superfamily

CDI family

Alternative Names

CAP20cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; CDKN1A; CDKN1melanoma differentiation associated protein 6; CIP1; CIP1WAF1CDK-interaction protein 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); MDA6; MDA-6; Melanoma differentiation-associated protein 6; p21; p21CIP1; p21Cip1/Waf1; PIC1; SDI1DNA synthesis inhibitor; wild-type p53-activated fragment 1 CDKN1A CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1 cyclin dependent kinase inhibitor 1A cyclin-dependent kinase inhibitor 1|CDK-interacting protein 1|CDK-interaction protein 1|DNA synthesis inhibitor|cyclin-dependent kinase inhibitor 1A (p21, Cip1)|melanoma differentiation associated protein 6|wild-type p53-activated fragment 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CDKN1A, check out the CDKN1A Infographic

CDKN1A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CDKN1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00145-3 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Chen Q,Fu L,Hu J,Guo G,Xie A.Silencing of PSMC2 inhibits development and metastasis of prostate cancer through regulating proliferation, apoptosis and migration.Cancer Cell Int.2021 Apr 26;21(1):235.doi:10.1186/s12935-021-01934-8.PMID:33902600;PMCID:PMC8077794.
Species: Human,Mouse
M00145-3 usage in article: APP:IHC, SAMPLE:PROSTATE CANCER TISSUE, DILUTION:NA

Minicircle-oriP-IFN?: A Novel Targeted Gene Therapeutic System for EBV Positive Human Nasopharyngeal Carcinoma

Action Mechanism of Inhibin ?-Subunit on the Development of Sertoli Cells and First Wave of Spermatogenesis in Mice

Wu K, Liu Y, Lv Y, Cui L, Li W, Chen J, Liang Nc, Li L. Oncol Rep. 2013 Jun;29(6):2101-8. Doi: 10.3892/Or.2013.2375. Epub 2013 Apr 3. Ent-11??-Hydroxy-15-Oxo-Kaur-16-En-19-Oic-Acid Induces Apoptosis And Cell Cycle Arrest In Cne-2Z Nasopharyngeal C...

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1 Customer Q&As for Anti-p21 CDKN1A Rabbit Monoclonal Antibody

Question

We are currently using anti-p21 Rabbit Monoclonal antibody M00145-3 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-24

Answer

The anti-p21 Rabbit Monoclonal antibody (M00145-3) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-24

Order DetailsPrice
M00145-3

100μL

$370
M00145-3-30ul

30μL sample

$99
M00145-3-carrier-free

Carrier Free

$370

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