Product Info Summary
SKU: | M04136-1 |
---|---|
Size: | 100ug |
Reactive Species: | Chicken, Guinea pig, Human, Mouse, Rabbit, Yeast |
Host: | Mouse |
Application: | ELISA, IP, IF, IHC, ICC, WB, Antibody Microarray |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-p23 Monoclonal Antibody
View all p23/PTGES3 Antibodies
SKU/Catalog Number
M04136-1
Size
100ug
Form
liquid
Description
Boster Bio Anti-p23 Monoclonal Antibody catalog # M04136-1. Tested in ELISA, IP, IF, IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-p23 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04136-1)
Host
Mouse
Contents
PBS, 50% glycerol, 0.09% sodium azide
Clonality
Monoclonal
Clone Number
JJ6
Isotype
IgG1
Immunogen
Recombinant human full length p23 protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~23kDa.
Reactive Species
M04136-1 is reactive to PTGES3 in Chicken, Guinea pig, Human, Mouse, Rabbit, Yeast
Applications
M04136-1 is guaranteed for ELISA, IP, IF, IHC, ICC, WB, Antibody Microarray Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
18.697kDa
Background of p23/PTGES3
p23 is a highly conserved ubiquitous protein, known to have an important function as a cochaperone for the HSP90 chaperoning system (1). Studies have revealed that p23 is a small protein (18 to 25 kDa) with a simple structure (2, 3). p23 does not have any structural homology with any other known proteins (1). p23 was first discovered as a part of the HSP90-progesterone receptor complex along with HSP70, p54 and p50 (1). p23 is a phosphor-protein, which is highly acidic and has an aspartic acid-rich c-terminal domain (1). Numerous studies have found p23 to be associated with other client proteins like Fes tyrosine kinase (4), the heme regulated kinase HRI (5), hsf1 transcription factor (4), aryl hydrocarbon receptor (4), telomerase (6), and Hepadnavirus reverse transcriptase (7). In spite of several years of study, the exact functional significance of p23 is still not clear (8). p23 is thought to be involved in the adenosine triphosphate–mediated HSP90 binding of client proteins (8). Since many HSP90 client proteins are involved in oncogenic survival signaling, a recent study has concluded p23 to be a promising target in leukemic apoptosis (9). HSP90 and its co-chaperone p23 are certainly among the emerging anti-tumor targets in oncology.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB (1:2000) IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Validation Images & Assay Conditions
Click image to see more details
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-p23 Monoclonal Antibody, Clone JJ6 (M04136-1) . Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-p23 Monoclonal Antibody (M04136-1) at 1:100 for cell-cell borders.
Click image to see more details
Figure 2. IHC analysis of PTGES3 using anti-PTGES3 antibody (M04136-1).
PTGES3 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PTGES3 Antibody (M04136-1) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
Click image to see more details
Figure 3. Western blot analysis of PTGES3 using anti-PTGES3 antibody (M04136-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGES3 antigen affinity purified polyclonal antibody (Catalog # M04136-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for PTGES3.
Click image to see more details
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-p23 Monoclonal Antibody, Clone JJ6 (M04136-1) . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-p23 Monoclonal Antibody (M04136-1) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-p23 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Protein Target Info & Infographic
Gene/Protein Information For PTGES3 (Source: Uniprot.org, NCBI)
Gene Name
PTGES3
Full Name
Prostaglandin E synthase 3
Weight
18.697kDa
Superfamily
p23/wos2 family
Alternative Names
CPGES; cPGESp23; Cytosolic prostaglandin E2 synthase; Hsp90 co-chaperone; p23; P23cytosolic prostaglandin E synthase; Progesterone receptor complex p23; prostaglandin E synthase 3 (cytosolic); prostaglandin E synthase 3; PTGES3; TEBP; TEBPEC 5.3.99.3; Telomerase-binding protein p23; unactive progesterone receptor, 23 kD PTGES3 P23, TEBP, cPGES prostaglandin E synthase 3 prostaglandin E synthase 3|Hsp90 co-chaperone|cytosolic prostaglandin E synthase|cytosolic prostaglandin E2 synthase|progesterone receptor complex p23|prostaglandin E synthase 3 (cytosolic)|telomerase-binding protein p23|unactive progesterone receptor, 23 kD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTGES3, check out the PTGES3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTGES3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p23 Monoclonal Antibody (M04136-1)
Hello CJ!
No publications found for M04136-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-p23 Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-p23 Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question