Data & Images
|Product Name||Anti-p27Kip1 Antibody (Monoclonal, DCS-72)|
|Description||Mouse IgG monoclonal antibody for p27Kip1, cyclin-dependent kinase inhibitor 1B (p27, Kip1) (CDKN1B) detection. Tested with WB, ICC in Human;mouse;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-p27Kip1 Antibody (Monoclonal, DCS-72) DCS-72 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1076)|
|Replacement Item||This antibody may replace the following items: sc-12939-R|sc-130603|sc-16324|sc-16324-R|sc-1641|sc-393305|sc-393380|sc-527|sc-528|sc-53871|sc-53906|sc-56338|sc-56454|sc-71813|sc-776 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||recombinant rodent p27Kip1 protein.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Cyclin-dependent kinase inhibitor 1B|
|Molecular Weight||22139 MW|
|Protein Function||CDKN1B: Important regulator of cell cycle progression. Involvedin G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2complexes. Forms a complex with cyclin type D-CDK4 complexes andis involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or anactivator of cyclin type D-CDK4 complexes depending on itsphosphorylation state and/or stoichometry.|
|Tissue Specificity||CDKN1B: Expressed in all tissues tested. Highestlevels in skeletal muscle, lowest in liver and kidney.|
|Sequence Similarities||Belongs to the CDI family.|
|Subcellular Localization||CDKN1B: Nucleus. Cytoplasm. Endosome Note: Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates tothe cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results intranslocation to the cytoplasm and cell cycle progression.Phosphorylation on Ser-10 facilitates nuclear export. Translocatesto the nucleus on phosphorylation of Tyr-88 and Tyr-89.Colocalizes at the endosome with SNX6; this leads to lysosomaldegradation (By similarity).
Cdkn1b: Nucleus. Cytoplasm. Endosome. Note: Nuclearand cytoplasmic in quiescent cells. AKT- or RSK-mediatedphosphorylation on Thr-197, binds 14-3-3, translocates to thecytoplasm and promotes cell cycle progression. Mitogen-activatedUHMK1 phosphorylation on Ser-10 also results in translocation tothe cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus onphosphorylation of Tyr-88 and Tyr-89 (By similarity). Colocalizesat the endosome with SNX6; this leads to lysosomal degradation.
|Alternative Names||Cyclin dependent kinase inhibitor ;Cyclin kinase inhibitor ;Cyclin-dependent kinase inhibitor 1B ;P27 kip1 ;P27kip1 ;Protein Cdkn1b ;Cdkn1b ;p27Kip1 ;rCG_29991 ;|
|Research Areas|||cell biology|cell cycle|cell cycle inhibitors|cip / kip| epigenetics and nuclear signaling|cip/kip| cancer||
Background for Cyclin-dependent kinase inhibitor 1B
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-p27Kip1 Antibody (Monoclonal, DCS-72) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -|
Western blot, 2-4μg/ml, Human, mouse, rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.