Anti-p38 MAPK14 Antibody

Boster Bio Anti-p38 MAPK14 Antibody catalog # A00176. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: A00176
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-p38 MAPK14 Antibody

See all p38 alpha products

SKU/Catalog Number

A00176

Size

100μl

Form

Liquid

Description

Boster Bio Anti-p38 MAPK14 Antibody catalog # A00176. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-p38 MAPK14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00176)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Immunogen

Synthesized peptide derived from human p38 around the non-phosphorylation site of Y323.

Reactive Species

A00176 is reactive to MAPK14 in Human, Mouse, Rat

Applications

A00176 is guaranteed for WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For MAPK14 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q16539

Gene ID

1432

Gene Name

MAPK14

Full Name

Mitogen-activated protein kinase 14

Weight

41.293kDa

Superfamily

protein kinase superfamily

Alternative Names

MAPK14; p38 alpha; SAPK2a MAPK14 CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA mitogen-activated protein kinase 14 mitogen-activated protein kinase 14|CSAID-binding protein|MAP kinase 14|MAP kinase Mxi2|MAP kinase p38 alpha|MAX-interacting protein 2|cytokine suppressive anti-inflammatory drug binding protein|mitogen-activated protein kinase p38 alpha|p38 MAP kinase|p38 mitogen activated protein kinase|p38alpha Exip|stress-activated protein kinase 2A

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MAPK14, check out the MAPK14 Infographic

MAPK14 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MAPK14: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00176 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ma G,Kimatu BM,Yang W,Pei F,Zhao L,Du H,Su A,Hu Q,Xiao H.Preparation of newly identified polysaccharide from Pleurotus eryngii and its anti-inflammation activities potential.J Food Sci.2020 Sep;85(9):2822-2831. doi:10.1111/1750-3841.15375.Epub 2020 Aug 14
Species: Mouse
A00176 usage in article: APP:WB, SAMPLE:RAW264.7 CELL, DILUTION:NA

Vasopressin decreases neuronal apoptosis during cardiopulmonary resuscitation

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15 Customer Q&As for Anti-p38 MAPK14 Antibody

Question

Is a blocking peptide available for product anti-p38 antibody (A00176)?

Verified Customer

Verified customer

Asked: 2020-05-01

Answer

We do provide the blocking peptide for product anti-p38 antibody (A00176). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-05-01

Question

Is this A00176 anti-p38 antibody reactive to the isotypes of MAPK14?

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

The immunogen of A00176 anti-p38 antibody is Synthesized peptide derived from human p38 around the non-phosphorylation site of Y323. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-14

Question

I have a question about product A00176, anti-p38 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-11-29

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00176 anti-p38 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-11-29

Question

Would A00176 anti-p38 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-11-19

Answer

It shows on the product datasheet, A00176 anti-p38 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-11-19

Question

Do you have a BSA free version of anti-p38 antibody A00176 available?

Verified Customer

Verified customer

Asked: 2019-09-13

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-p38 antibody A00176 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-09-13

Question

Please see the WB image, lot number and protocol we used for t-cell using anti-p38 antibody A00176. Please let me know if you require anything else.

J. Roberts

Verified customer

Asked: 2019-09-11

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-11

Question

We are currently using anti-p38 antibody A00176 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-01

Answer

The anti-p38 antibody (A00176) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-01

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-p38 antibody A00176. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-14

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-14

Question

I see that the anti-p38 antibody A00176 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-02-07

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-02-07

Question

Does anti-p38 antibody A00176 work for WB with t-cell?

Verified Customer

Verified customer

Asked: 2018-03-08

Answer

According to the expression profile of t-cell, MAPK14 is highly expressed in t-cell. So, it is likely that anti-p38 antibody A00176 will work for WB with t-cell.

Boster Scientific Support

Answered: 2018-03-08

Question

We want using your anti-p38 antibody for dscam interactions studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-11-29

Answer

Thank you for your inquiry. This A00176 anti-p38 antibody is tested on hela cells. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-11-29

Question

We have observed staining in human embryonic kidney. Do you have any suggestions? Is anti-p38 antibody supposed to stain embryonic kidney positively?

V. Singh

Verified customer

Asked: 2017-11-09

Answer

According to literature embryonic kidney does express MAPK14. According to Uniprot.org, MAPK14 is expressed in blood, peripheral blood, liver, b-cell, renal cell carcinoma, placenta, platelet, hepatoma, embryonic kidney, cervix carcinoma, leukemic t-cell, t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MAPK14 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 10727080
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Hepatoma, Pubmed ID: 10943842
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 7696354, 24275569
Peripheral blood, Pubmed ID: 7997261
Placenta, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801, 18088087
Renal cell carcinoma, Pubmed ID: 11866441
T-cell, Pubmed ID: 21586573

Boster Scientific Support

Answered: 2017-11-09

Question

Our team were satisfied with the WB result of your anti-p38 antibody. However we have observed positive staining in renal cell carcinoma cytoplasm. using this antibody. Is that expected? Could you tell me where is MAPK14 supposed to be expressed?

M. Lewis

Verified customer

Asked: 2017-07-14

Answer

Based on literature, renal cell carcinoma does express MAPK14. Generally MAPK14 expresses in cytoplasm. Regarding which tissues have MAPK14 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 10727080
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Hepatoma, Pubmed ID: 10943842
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 7696354, 24275569
Peripheral blood, Pubmed ID: 7997261
Placenta, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801, 18088087
Renal cell carcinoma, Pubmed ID: 11866441
T-cell, Pubmed ID: 21586573

Boster Scientific Support

Answered: 2017-07-14

Question

Our lab want to know about to test anti-p38 antibody A00176 on human t-cell for research purposes, then I may be interested in using anti-p38 antibody A00176 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-06-21

Answer

The products we sell, including anti-p38 antibody A00176, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-06-21

Question

I was wanting to use your anti-p38 antibody for WB for human t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human t-cell identification?

S. Roberts

Verified customer

Asked: 2013-05-15

Answer

You can see on the product datasheet, A00176 anti-p38 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-05-15

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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A00176
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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