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Boster offers custom polyclonal antibody production for researchers who use non-mammalian models such as Zebrafish, Drophila, C. elegans and Yeast at $600. Contact us for a free consultation.
Boster offers high quality custom antibody production services, including Rabbit and Mouse monoclonal, as well as rabbit polyclonal. For Hu, Mo and Ra targets, we provide Immunoassay development service.For non-hu-mo-ra targets,take advantage of our $600 rare species custom polyclonal program.
Boster provides plate-based multiplex cytokine immunoassay service for analytes from human, mouse and rat. Contact us today and get a free consultation.
Boster offers custom recombinant protein expression service. Available expresssion systems include E. Coli, Yeast, Insect and Mamalian Cells. Get a free consultation today.
Boster offers custom DNA synthesis service for as low as $0.08 per bp. Any gene cloning into any vector, 100% accuracy, Fast turn around time. Get a free consultation today.
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Breast Cancer Regulation
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The following reagents are used to generate the images below.
ChIP assays were performed using human U2OS cells, treated with camptothecin, Anti-p53 polyclonal antibody (Catalog # CI1060) and optimized PCR primer sets for QPCR. ChIP was performed on sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 ??g per ChIP experiment was analysed. IgG (2 ??g/IP) was used as negative IP control. QPCR was performed with primers for the p21 and GAS6 genes used as positive controls, and for GAPDH gene and the Sat2
satellite repeat, used as negative controls.
ChIP was performed on sheared chromatin from 4 million U2OS cells using 1 μg of Anti-p53 polyclonal antibody (Catalog # CI1060). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the X-chromosome (fig 2A) and in 3 genomic regions of chromosome 6, 13 and 12, surrounding p21 (CDKN1A), GAS6 and MDM2, 3 known targets genes of p53 (fig 2B, C and D, respectively).
To determine the titer of the antibody, an ELISA was performed using a serial dilution of Anti-p53 polyclonal antibody (Catalog # CI1060), crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:308,000.
Western blot analysis of p53 expression in nuclear extracts from HeLa cells (40 µg). p53 was detected using Anti-p53 polyclonal antibody (Catalog # CI1060) at 1/2000 dilution.
*if product is indicated to react with multiple species, protein info is based on the human gene.
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