Product Info Summary
SKU: | CI1060 |
---|---|
Size: | 50μg/50μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ChIP, ChIP-seq, ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-p53 TP53 Antibody
SKU/Catalog Number
CI1060
Size
50μg/50μl
Form
Liquid
Description
Boster Bio Anti-p53 TP53 Antibody (Catalog# CI1060). Tested in ChIP, ChIP-seq, ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C. For long-term storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Cite This Product
Anti-p53 TP53 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # CI1060)
Host
Rabbit
Contents
Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.
Clonality
Polyclonal
Immunogen
This antibody is raised in rabbit against human p53 (tumor protein p53), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
CI1060 is reactive to TP53 in Human
Applications
CI1060 is guaranteed for ChIP, ChIP-seq, ELISA, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
43.653kDa
Background of p53
The transcription factor p53 is a tumour suppressor that regulates the cellular response to diverse cellular stresses. Upon activation, p53 induces several target genes which leads to cell cycle arrest and DNA repair, or alternatively, to apoptosis. In unstressed cells, p53 is kept inactive by the ubiquitin ligase MDM2 which inhibits the activity and promotes the degradation. Mutations in p53 are involved in a vast majority of human cancers.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
Click image to see more details
ChIP assays were performed using human U2OS cells, treated with camptothecin, Anti-p53 polyclonal antibody (Catalog # CI1060) and optimized PCR primer sets for QPCR. ChIP was performed on sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. QPCR was performed with primers for the p21 and GAS6 genes used as positive controls, and for GAPDH gene and the Sat2 satellite repeat, used as negative controls.
Click image to see more details
To determine the titer of the antibody, an ELISA was performed using a serial dilution of Anti-p53 polyclonal antibody (Catalog # CI1060), crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:308,000.
Click image to see more details
ChIP was performed on sheared chromatin from 4 million U2OS cells using 1 μg of Anti-p53 polyclonal antibody (Catalog # CI1060). The IP DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the X-chromosome (fig 2A) and in 3 genomic regions of chromosome 6, 13 and 12, surrounding p21 (CDKN1A), GAS6 and MDM2, 3 known targets genes of p53 (fig 2B, C and D, respectively).
Click image to see more details
Western blot analysis of p53 expression in nuclear extracts from HeLa cells (40
Protein Target Info & Infographic
Gene/Protein Information For TP53 (Source: Uniprot.org, NCBI)
Gene Name
TP53
Full Name
Cellular tumor antigen p53
Weight
43.653kDa
Superfamily
p53 family
Alternative Names
Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53 TP53 BCC7, BMFS5, LFS1, P53, TRP53 tumor protein p53 cellular tumor antigen p53|antigen NY-CO-13|mutant tumor protein 53|p53 tumor suppressor|phosphoprotein p53|transformation-related protein 53|tumor protein 53|tumor supressor p53
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TP53, check out the TP53 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TP53: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-p53 TP53 Antibody (CI1060)
Hello CJ!
No publications found for CI1060
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-p53 TP53 Antibody?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-p53 TP53 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-p53 TP53 Antibody
Question
Please see the WB image, lot number and protocol we used for glial cell glial tumor using anti-p53 antibody CI1060. Please let me know if you require anything else.
A. Mangal
Verified customer
Asked: 2020-04-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-07
Question
Is there a BSA free version of anti-p53 antibody CI1060 available?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-p53 antibody CI1060 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-17
Question
We want using your anti-p53 antibody for signal transduction by p53 class mediator resulting in transcription of p21 class mediator studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
H. Li
Verified customer
Asked: 2019-12-05
Answer
We appreciate your inquiry. This CI1060 anti-p53 antibody is tested on human u2os cells, million cells, hela cells. It is guaranteed to work for ChIP, ChIP-qPCR, ChIP-seq, Flow Cytometry, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-05
Question
My question regards to test anti-p53 antibody CI1060 on human glial cell glial tumor for research purposes, then I may be interested in using anti-p53 antibody CI1060 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
The products we sell, including anti-p53 antibody CI1060, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-22
Question
Is this CI1060 anti-p53 antibody reactive to the isotypes of TP53?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
The immunogen of CI1060 anti-p53 antibody is This antibody is raised in rabbit against human p53 (tumor protein p53), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-13
Question
Would anti-p53 antibody CI1060 work for IHC with glial cell glial tumor?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
According to the expression profile of glial cell glial tumor, TP53 is highly expressed in glial cell glial tumor. So, it is likely that anti-p53 antibody CI1060 will work for IHC with glial cell glial tumor.
Boster Scientific Support
Answered: 2019-08-27
Question
We ordered your anti-p53 antibody for ChIP on colon last year. I am using human, and We intend to use the antibody for ChIP-seq next. you antibody examining colon as well as lung carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for ChIP-seq?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
I have checked the website and datasheets of our anti-p53 antibody and I see that CI1060 has been validated on human in both ChIP and ChIP-seq. Thus CI1060 should work for your application. Our Boster satisfaction guarantee will cover this product for ChIP-seq in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ChIP-seq detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-08-19
Question
Our lab were happy with the WB result of your anti-p53 antibody. However we have been able to see positive staining in ovarian adenocarcinoma cytoplasm using this antibody. Is that expected? Could you tell me where is TP53 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-04-17
Answer
Based on literature, ovarian adenocarcinoma does express TP53. Generally TP53 expresses in cytoplasm. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794
Boster Scientific Support
Answered: 2019-04-17
Question
Would CI1060 anti-p53 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Krishna
Verified customer
Asked: 2018-12-27
Answer
As indicated on the product datasheet, CI1060 anti-p53 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-12-27
Question
We have been able to see staining in human lung carcinoma. Any tips? Is anti-p53 antibody supposed to stain lung carcinoma positively?
Verified Customer
Verified customer
Asked: 2018-10-22
Answer
From what I have seen in literature lung carcinoma does express TP53. From what I have seen in Uniprot.org, TP53 is expressed in epithelium of mammary gland, colon, kidney, lung carcinoma, blood, ovarian adenocarcinoma, glial cell glial tumor, embryonic kidney, among other tissues. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794
Boster Scientific Support
Answered: 2018-10-22
Question
I was wanting to use your anti-p53 antibody for IHC for human glial cell glial tumor on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human glial cell glial tumor identification?
Verified Customer
Verified customer
Asked: 2018-03-14
Answer
You can see on the product datasheet, CI1060 anti-p53 antibody has been tested for ChIP, ChIP-qPCR, ChIP-seq, Flow Cytometry, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human glial cell glial tumor in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-14
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for glial cell glial tumor using anti-p53 antibody CI1060. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-01-05
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-05
Question
I see that the anti-p53 antibody CI1060 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-11-29
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-11-29
Question
Can you help my question with product CI1060, anti-p53 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-13
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free CI1060 anti-p53 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-13
Question
We are currently using anti-p53 antibody CI1060 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
V. Patel
Verified customer
Asked: 2017-05-08
Answer
The anti-p53 antibody (CI1060) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-08
Question
Is a blocking peptide available for product anti-p53 antibody (CI1060)?
R. Gonzalez
Verified customer
Asked: 2016-10-04
Answer
We do provide the blocking peptide for product anti-p53 antibody (CI1060). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-10-04