SKU CI1060
Size 50μg/50μl
Reactivity Human
Clonality Polyclonal
Host Rabbit
Applications ChIP, ChIP-qPCR, ChIP-seq, Flow Cytometry, IHC, WB

Overview

Product Name Anti-p53 Antibody
SKU/Catalog Number CI1060
Storage & Handling At -20°C for one year, at 4°C for one month. Avoid repeated freezing and thawing.
Size 50μg/50μl
Description Rabbit IgG polyclonal antibody for human p53 (tumor protein p53).tested for ChIP, ChIP-qPCR, ChIP-seq, Flow Cytometry, IHC, WB in Human.
Cite This Product Anti-p53 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # CI1060)
Host Rabbit
Contents/Buffer Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.
Form Liquid
Immunogen This antibody is raised in rabbit against human p53 (tumor protein p53), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.
Reactivity Human

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
User need to optimize the dilution ratio for this antibody

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. Boster recommends high sensitivity ChIP-seq Kit (CK1001 & CK1002) for Chromatin Immunoprecipitation.

Images And Assay Conditions

/antibody/ci1060_chip_qpcr anti p53 antibody.jpg

ChIP assays were performed using human U2OS cells, treated with camptothecin, Anti-p53 polyclonal antibody (Catalog # CI1060) and optimized PCR primer sets for QPCR. ChIP was performed on sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2, 5, and 10 ??g per ChIP experiment was analysed. IgG (2 ??g/IP) was used as negative IP control. QPCR was performed with primers for the p21 and GAS6 genes used as positive controls, and for GAPDH gene and the Sat2 satellite repeat, used as negative controls.

/antibody/ci1060_chip_seq anti p53 antibody.jpg

ChIP was performed on sheared chromatin from 4 million U2OS cells using 1 μg of Anti-p53 polyclonal antibody (Catalog # CI1060). The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the X-chromosome (fig 2A) and in 3 genomic regions of chromosome 6, 13 and 12, surrounding p21 (CDKN1A), GAS6 and MDM2, 3 known targets genes of p53 (fig 2B, C and D, respectively).

/antibody/ci1060_elisa anti p53 antibody.jpg

To determine the titer of the antibody, an ELISA was performed using a serial dilution of Anti-p53 polyclonal antibody (Catalog # CI1060), crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:308,000.

/antibody/ci1060_wb anti p53 antibody.jpg

Western blot analysis of p53 expression in nuclear extracts from HeLa cells (40 µg). p53 was detected using Anti-p53 polyclonal antibody (Catalog # CI1060) at 1/2000 dilution.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P04637
Gene Name TP53
Protein Name Cellular tumor antigen p53
Tissue Specificity Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine. .
Alternative Names Cellular tumor antigen p53;Antigen NY-CO-13;Phosphoprotein p53;Tumor suppressor p53;TP53;P53;
Subcellular Localization Cytoplasm. Nucleus. Nucleus, PML body. Endoplasmic reticulum. Mitochondrion matrix. Interaction with BANP promotes nuclear localization. Recruited into PML bodies together with CHEK2. Translocates to mitochondria upon oxidative stress.
Molecular Weight 43653 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA- Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1- mediated transcriptional activation of PER2 (PubMed:24051492). .
Research Areas Apoptosis, Cell Biology, Intracellular, P53 Pathway

*You can search these to find other products in these research areas.
Background The transcription factor p53 is a tumour suppressor that regulates the cellular response to diverse cellular stresses. Upon activation, p53 induces several target genes which leads to cell cycle arrest and DNA repair, or alternatively, to apoptosis. In unstressed cells, p53 is kept inactive by the ubiquitin ligase MDM2 which inhibits the activity and promotes the degradation. Mutations in p53 are involved in a vast majority of human cancers.

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