|Application:||IHC-P, IHC-F, ICC, WB|
Data & Images
|Product Name||Anti-P53 Antibody|
|Description||Mouse IgG monoclonal antibody for p53, tumor protein p53 (TP53) detection. Tested with WB, IHC-P, IHC-F, ICC in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-P53 Antibody BP53-12 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1078)|
|Replacement Item||This antibody may replace the following items: sc-100350|sc-10840|sc-10843|sc-126|sc-126-X|sc-12904-R|sc-1311-R|sc-135772|sc-135772-X|sc-135773|sc-135773-X|sc-136832|sc-137173|sc-137174|sc-137175|sc-139542|sc-139543|sc-16715-R|sc-16716-R|sc-17105-R|sc-17106-R|sc-17274-R|sc-18079-R|sc-21872-R|sc-22785|sc-244647|sc-244648|sc-249070|sc-249071|sc-376973|sc-398705|sc-48262|sc-514582|sc-514703|sc-6243-G|sc-6243-X|sc-67184|sc-71783|sc-71784|sc-71785|sc-71786|sc-71817-X|sc-7997-R|sc-7997-X|sc-81511|sc-85846|sc-85848|sc-85975|sc-98-X|sc-98384 from Santa Cruz Biotechnology.|
|Application||IHC-P, IHC-F, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Recombinant human wild-type p53 protein.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Cellular tumor antigen p53|
|Molecular Weight||43451 MW|
|Protein Function||Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA- Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2. .|
|Tissue Specificity||TP53: Ubiquitous. Isoforms are expressed in a widerange of normal tissues but in a tissue-dependent manner. Isoform2 is expressed in most normal tissues but is not detected inbrain, lung, prostate, muscle, fetal brain, spinal cord and fetalliver. Isoform 3 is expressed in most normal tissues but is notdetected in lung, spleen, testis, fetal brain, spinal cord andfetal liver. Isoform 7 is expressed in most normal tissues but isnot detected in prostate, uterus, skeletal muscle and breast.Isoform 8 is detected only in colon, bone marrow, testis, fetalbrain and intestine. Isoform 9 is expressed in most normal tissuesbut is not detected in brain, heart, lung, fetal liver, salivarygland, breast or intestine.|
|Sequence Similarities||Belongs to the p53 family.|
|Subcellular Localization||Cytoplasm . Nucleus . Endoplasmic reticulum . Mitochondrion matrix . Interaction with BANP promotes nuclear localization. Translocates to mitochondria upon oxidative stress (By similarity). .|
|Alternative Names||Cellular tumor antigen p53;Tumor suppressor p53;Tp53;P53;|
|Research Areas|||cell biology|apoptosis|intracellular|p53 pathway||
Background for Cellular tumor antigen p53
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-P53 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-2μg/ml, Human, By Heat
Immunocytochemistry , 1μg/ml, Human, -
Immunohistochemistry(Frozen Section), 0.5-2μg/ml, Human, -
Western blot, 0.5-1μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-P53 Antibody Images
Click the images to enlarge.
WB: HL-60 Cell Lysate
IHC(P): Human Mammary Cancer Tissue