Anti-Phospho-p53 (Ser392) TP53 Antibody

p53 antibody

Boster Bio Anti-Phospho-p53 (Ser392) TP53 Antibody (Catalog # P00001). Tested in WB applications. This antibody reacts with Human, Rat. Cited in 4 publication(s).

Product Info Summary

SKU: P00001
Size: 100 µl
Reactive Species: Human, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Phospho-p53 (Ser392) TP53 Antibody

View all p53 Antibodies

SKU/Catalog Number

P00001

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-p53 (Ser392) TP53 Antibody (Catalog # P00001). Tested in WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-p53 (Ser392) TP53 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00001)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Clone Number

JE44-80

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser392 of human p53, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P00001 is reactive to TP53 in Human, Rat

Applications

P00001 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

43.653kDa

Background of p53

p53 has a well established role in blocking the proliferative action of damaged cells and acting in essence as an anticancer agent (Sharpless and DePinho, 2002; Yin et al., 1992). It has been called the guardian of the genome (Lane, 1992). Phosphorylation of Ser-392 in p53 is associated with formation of human tumors (Saito et al., 2003; Pise-Masison et al., 1998; Kim et al., 2004). In addition, p53 has been linked to effects of aging and oxidative stress (Sharpless and DePinho, 2002). An increase in p53 has also been linked to deficits in LTP and learning and memory (Jiang et al., 1998)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

Gene/Protein Information For TP53 (Source: Uniprot.org, NCBI)

Gene Name

TP53

Full Name

Cellular tumor antigen p53

Weight

43.653kDa

Superfamily

p53 family

Alternative Names

Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53 TP53 BCC7, BMFS5, LFS1, P53, TRP53 tumor protein p53 cellular tumor antigen p53|antigen NY-CO-13|mutant tumor protein 53|p53 tumor suppressor|phosphoprotein p53|transformation-related protein 53|tumor protein 53|tumor supressor p53

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TP53, check out the TP53 Infographic

TP53 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TP53: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

P00001 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

P-glycoprotein Mediates Postoperative Peritoneal Adhesion Formation by Enhancing Phosphorylation of the Chloride Channel-3

Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats.

Effect of ligustilide on Ang II-induced hypertrophy in cardiomyocytes and the potential mechanisms

Zhang D, Fu M, Song C, Wang C, Lin X, Liu Y. Neural Regen Res. 2012 Oct 25;7(30):2347-53. Doi: 10.3969/J.Issn.1673-5374.2012.30.004. Expressions Of Apoptosis-Related Proteins In Rats With Focal Cerebral Ischemia After Angong Niuhuang Sticker Point...

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3 Customer Q&As for Anti-Phospho-p53 (Ser392) TP53 Antibody

Question

My colleagues were happy with the WB result of your anti-Phospho-p53 (Ser392) antibody. However we have been able to see positive staining in glial cell glial tumor cytoplasm using this antibody. Is that expected? Could you tell me where is TP53 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-08-31

Answer

From what I have seen in literature, glial cell glial tumor does express TP53. Generally TP53 expresses in cytoplasm. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794

Boster Scientific Support

Answered: 2017-08-31

Question

We are currently using anti-Phospho-p53 (Ser392) antibody P00001 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says rat. Is it likely that the antibody can work on canine tissues as well?

P. Jackson

Verified customer

Asked: 2014-09-04

Answer

The anti-Phospho-p53 (Ser392) antibody (P00001) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-09-04

Question

We have seen staining in rat ovarian adenocarcinoma. Are there any suggestions? Is anti-Phospho-p53 (Ser392) antibody supposed to stain ovarian adenocarcinoma positively?

J. Roberts

Verified customer

Asked: 2014-03-12

Answer

According to literature ovarian adenocarcinoma does express TP53. According to Uniprot.org, TP53 is expressed in epithelium of mammary gland, colon, kidney, lung carcinoma, blood, ovarian adenocarcinoma, glial cell glial tumor, embryonic kidney, among other tissues. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794

Boster Scientific Support

Answered: 2014-03-12

Order DetailsPrice
P00001

100uL

$465

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