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SKU:PA2304
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-TNFRSF16/NGFR Antibody
SKU/Catalog Number PA2304
Description Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 16(NGFR) detection. Tested with WB in Human;Mouse;Rat.
Cite This Product Anti-TNFRSF16/NGFR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2304)
Replacement Item This antibody may replace the following items: sc-13577|sc-25283|sc-271708|sc-53631|sc-55467|sc-5634|sc-56448|sc-58567|sc-6188|sc-6188-R|sc-6189|sc-71691|sc-71692|sc-71693|sc-71694|sc-71695|sc-8317 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human p75 NGF Receptor(397-414aa TLDALLAALRRIQRADLV), different from the related mouse and rat sequences by one amino acid.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name NGFR
Protein Name Tumor necrosis factor receptor superfamily member 16
Molecular Weight 45183 MW
Protein Function Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. .
Sequence Similarities Contains 1 death domain.
Subcellular Localization Membrane; Single-pass type I membrane protein.
Uniprot ID P08138
Alternative Names Tumor necrosis factor receptor superfamily member 16;Gp80-LNGFR;Low affinity neurotrophin receptor p75NTR;Low-affinity nerve growth factor receptor;NGF receptor;p75 ICD;CD271;NGFR;TNFRSF16;
Research Areas |neuroscience|neurology process|growth and development|neurotrophins| stem cells|neural stem cells|surface molecules|mesenchymal stem cells|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Tumor necrosis factor receptor superfamily member 16

The Low-Affinity Nerve Growth Factor Receptor(also called the NGFR or p75 neurotrophin receptor) is one of the two receptor types for the neurotrophins, a family of protein growth factors that stimulate neuronal cells to survive and differentiate. It is mapped to 17q21.33. Overexpression of NGFR was also found to activate the SM pathway. NGFR is a receptor for rabies virus, and it is also a coreceptor for the NOGO receptor for MAG signaling. This gene is a signaling partner for EPHAs and the EPHA/NGFR complex reverse signals can mediate axon repulsion required for guidance and mapping. NGFR is involved in activity-dependent synaptic plasticity. It has been shown to interact with PRKACB, FSCN1, TRAF4, Nerve growth factor, TRAF2, NDN, NGFRAP1 and MAGEH1.

Anti-TNFRSF16/NGFR Antibody Images

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Anti-TNFRSF16/NGFR Antibody
Anti-p75 NGF Receptor antibody, PA2304, All Western blotting
All lanes: Anti-NGFR(PA2304) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: SHG Whole Cell Lysate at 40ug
Lane 3: NEURO Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Predicted bind size: 45KD
Observed bind size: 45KD
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Publications

Shen Y, Qian Y, Zhang H, Zuo B, Lu Z, Fan Z, Zhang P, Zhang F, Zhou C. Cell Transplant. 2010;19(2):147-57. Doi: 10.3727/096368910X492616. Epub 2010 Mar 26. Guidance Of Olfactory Ensheathing Cell Growth And Migration On Electrospun Silk Fibroin Sca...
Fan Z, Shen Y, Zhang F, Zuo B, Lu Q, Wu P, Xie Z, Dong Q, Zhang H. Cell Transplant. 2013;22 Suppl 1:S39-50. Doi: 10.3727/096368913X672190. Epub 2013 Oct 22. Control Of Olfactory Ensheathing Cell Behaviors By Electrospun Silk Fibroin Fibers.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to nerve growth factor receptor antibody, ngfr antibody, p75ntr antibody