Anti-PABPN1 Rabbit Monoclonal Antibody

PABPN1 antibody

Boster Bio Anti-PABPN1 Rabbit Monoclonal Antibody catalog # M02445. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: M02445
Size: 100 μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

Product Name

Anti-PABPN1 Rabbit Monoclonal Antibody

View all PABPN1 Antibodies

SKU/Catalog Number

M02445

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-PABPN1 Rabbit Monoclonal Antibody catalog # M02445. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PABPN1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02445)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AAHI-16

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human PABPN1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M02445 is reactive to PABPN1 in Human, Mouse

Applications

M02445 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

53 kDa

Calculated molecular weight

32.749kDa

Background of PABPN1

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For PABPN1 (Source: Uniprot.org, NCBI)

Gene Name

PABPN1

Full Name

Polyadenylate-binding protein 2

Weight

32.749kDa

Alternative Names

Nuclear poly(A)-binding protein 1; OPMD; PAB2poly(A) binding protein II; PABII; PABP-2; PABP2polyadenylate-binding protein 2; poly(A) binding protein 2; poly(A) binding protein, nuclear 1; Poly(A)-binding protein 2; Poly(A)-binding protein II; poly(A)-binding protein, nuclear 1; Polyadenylate-binding nuclear protein 1 PABPN1 OPMD, PAB2, PABII, PABP-2, PABP2 poly(A) binding protein nuclear 1 polyadenylate-binding protein 2|poly(A) binding protein 2|poly(A) binding protein II

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PABPN1, check out the PABPN1 Infographic

PABPN1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PABPN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-PABPN1 Rabbit Monoclonal Antibody

Question

We are currently using anti-PABPN1 Rabbit Monoclonal antibody M02445 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-13

Answer

The anti-PABPN1 Rabbit Monoclonal antibody (M02445) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-13

Question

Is this M02445 anti-PABPN1 Rabbit Monoclonal antibody reactive to the isotypes of PABPN1?

Verified Customer

Verified customer

Asked: 2020-01-16

Answer

The immunogen of M02445 anti-PABPN1 Rabbit Monoclonal antibody is A synthesized peptide derived from human PABPN1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-16

Question

Would anti-PABPN1 Rabbit Monoclonal antibody M02445 work for IF with leukemic t-cell?

Verified Customer

Verified customer

Asked: 2019-10-15

Answer

According to the expression profile of leukemic t-cell, PABPN1 is highly expressed in leukemic t-cell. So, it is likely that anti-PABPN1 Rabbit Monoclonal antibody M02445 will work for IF with leukemic t-cell.

Boster Scientific Support

Answered: 2019-10-15

Question

Here is the WB image, lot number and protocol we used for leukemic t-cell using anti-PABPN1 Rabbit Monoclonal antibody M02445. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-07-10

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-07-10

Question

Is a blocking peptide available for product anti-PABPN1 Rabbit Monoclonal antibody (M02445)?

G. Anderson

Verified customer

Asked: 2014-03-21

Answer

We do provide the blocking peptide for product anti-PABPN1 Rabbit Monoclonal antibody (M02445). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2014-03-21

Question

Can you help my question with product M02445, anti-PABPN1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

D. Jones

Verified customer

Asked: 2013-01-07

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02445 anti-PABPN1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-01-07

Order DetailsPrice
M02445

100μL

$370
M02445-30ul

30μL sample

$99
M02445-carrier-free

Carrier Free

$370

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Free Secondary Antibody

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M02445
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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