Anti-PAH Antibody Picoband™

Phenylalanine Hydroxylase antibody

Boster Bio Anti-PAH Antibody Picoband™ catalog # A00761-1. Tested in Flow Cytometry(Intracellular), IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: A00761-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB, Flow Cytometry (Intracellular)

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Product Name

Anti-PAH Antibody Picoband™

View all Phenylalanine Hydroxylase Antibodies

SKU/Catalog Number

A00761-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-PAH Antibody Picoband™ catalog # A00761-1. Tested in Flow Cytometry(Intracellular), IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PAH Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00761-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human PAH recombinant protein (Position: R71-H208). Human PAH shares 89.1% and 88.4% amino acid (aa) sequence identity with mouse and rat PAH, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00761-1 is reactive to PAH in Human, Mouse, Rat

Applications

A00761-1 is guaranteed for IHC, WB, Flow Cytometry (Intracellular) Boster Guarantee

Observed Molecular Weight

45 kDa

Calculated molecular weight

51.862kDa

Background of Phenylalanine Hydroxylase

Phenylalanine hydroxylase (PAH) is an enzyme that catalyzes the hydroxylation of the aromatic side-chain of phenylalanine to generate tyrosine. It is one of three members of the biopterin-dependent aromatic amino acid hydroxylases, a class of monooxygenase that uses tetrahydrobiopterin (BH4, a pteridine cofactor) and a non-heme iron for catalysis. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat,
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat
Flow Cytometry(Intracellular), 1-3 μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For PAH (Source: Uniprot.org, NCBI)

Gene Name

PAH

Full Name

Phenylalanine-4-hydroxylase

Weight

51.862kDa

Superfamily

biopterin-dependent aromatic amino acid hydroxylase family

Alternative Names

EC 1.14.16; EC 1.14.16.1; PH; phe-4-monooxygenase; phenylalanine 4-monooxygenase; phenylalanine hydroxylase; phenylalanine-4-hydroxylase; PKU; PKU1 PAH PH, PKU, PKU1 phenylalanine hydroxylase phenylalanine-4-hydroxylase|phe-4-monooxygenase|phenylalanine 4-monooxygenase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PAH, check out the PAH Infographic

PAH infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PAH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00761-1 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

CRISPR/Cas9 generated knockout mice lacking phenylalanine hydroxylase protein as a novel preclinical model for human phenylketonuria

Singh K,Cornell CS,Jackson R,Kabiri M,Phipps M,Desai M,Fogle R,Ying X,Anarat-Cappillino G,Geller S,Johnson J,Roberts E,Malley K,Devlin T,DeRiso M,Berthelette P,Zhang YV,Ryan S,Rao S,Thurberg BL,Bangari DS,Kyostio-Moore S.CRISPR/Cas9 generated knockout mice lacking phenylalanine hydroxylase protein as a novel preclinical model for human phenylketonuria.Sci Rep.2021 Mar 31;11(1):7254.doi:10.1038/s41598-021-86663-8.PMID:33790381.
Species: Mouse
A00761-1 usage in article: APP:IHC, SAMPLE:LIVER,KIDNEY AND BRAIN TISSUE, DILUTION:NA

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5 Customer Q&As for Anti-PAH Antibody Picoband™

Question

Will anti-PAH antibody A00761-1 work for WB with liver?

Verified Customer

Verified customer

Asked: 2020-05-05

Answer

According to the expression profile of liver, PAH is highly expressed in liver. So, it is likely that anti-PAH antibody A00761-1 will work for WB with liver.

Boster Scientific Support

Answered: 2020-05-05

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-PAH antibody A00761-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-03-27

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-27

Question

We are currently using anti-PAH antibody A00761-1 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-13

Answer

The anti-PAH antibody (A00761-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-13

Question

Will anti-PAH antibody A00761-1 work on horse IHC-F with nephron tubule?

Verified Customer

Verified customer

Asked: 2019-02-06

Answer

Our lab technicians have not validated anti-PAH antibody A00761-1 on horse. You can run a BLAST between horse and the immunogen sequence of anti-PAH antibody A00761-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse nephron tubule in IHC-F, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-02-06

Question

Is this A00761-1 anti-PAH antibody reactive to the isotypes of PAH?

L. Huang

Verified customer

Asked: 2014-05-15

Answer

The immunogen of A00761-1 anti-PAH antibody is E. coli-derived human PAH recombinant protein (Position: R71-H208). Human PAH shares 89.1% and 88.4% amino acid (aa) sequence identity with mouse and rat PAH, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-05-15

Order DetailsPrice
A00761-1

100μg

$370
A00761-1-10ug

10μg sample (liquid)

$99
A00761-1-Biotin

100 μg Biotin conjugated

$570
A00761-1-Cy3

100 μg Cy3 conjugated

$570
A00761-1-Dylight488

100 μg Dylight488 conjugated

$570
A00761-1-Dylight550

100 μg Dylight550 conjugated

$570
A00761-1-Dylight594

100 μg Dylight594 conjugated

$570
A00761-1-FITC

100 μg FITC conjugated

$570
A00761-1-HRP

100 μg HRP conjugated

$570
A00761-1-APC

100 μg APC conjugated

$670
A00761-1-PE

100 μg PE conjugated

$670
A00761-1-carrier-free

Carrier Free

$370

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A00761-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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