Anti-PAI1 Rabbit Monoclonal Antibody

Serpin E1/PAI-1 antibody

Boster Bio Anti-PAI1 Rabbit Monoclonal Antibody catalog # M00637. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: M00637
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IP, IF, ICC, WB

Product Name

Anti-PAI1 Rabbit Monoclonal Antibody

View all Serpin E1/PAI-1 Antibodies

SKU/Catalog Number

M00637

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-PAI1 Rabbit Monoclonal Antibody catalog # M00637. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PAI1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00637)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ABHG-19

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human PAI1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00637 is reactive to SERPINE1 in Human

Applications

M00637 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

85 kDa

Calculated molecular weight

45.06kDa

Background of Serpin E1/PAI-1

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:1000
ICC/IF 1:50-1:200
IP 1:30
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For SERPINE1 (Source: Uniprot.org, NCBI)

Gene Name

SERPINE1

Full Name

Plasminogen activator inhibitor 1

Weight

45.06kDa

Superfamily

serpin family

Alternative Names

Endothelial plasminogen activator inhibitor; Nexin; PAI1; PAI-1; PAI1PAI-1; PAISerpin E1; PLANH1; PLANH1plasminogen activator inhibitor 1; serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1; Serpin E1; serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1 SERPINE1 PAI, PAI-1, PAI1, PLANH1 serpin family E member 1 plasminogen activator inhibitor 1|endothelial plasminogen activator inhibitor|serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1|serpin E1|serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SERPINE1, check out the SERPINE1 Infographic

SERPINE1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SERPINE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for M00637

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-PAI1 Rabbit Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-PAI1 Rabbit Monoclonal Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

4 Customer Q&As for Anti-PAI1 Rabbit Monoclonal Antibody

Question

Does M00637 anti-PAI1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-06

Answer

It shows on the product datasheet, M00637 anti-PAI1 Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-06

Question

Is this M00637 anti-PAI1 Rabbit Monoclonal antibody reactive to the isotypes of SERPINE1?

Verified Customer

Verified customer

Asked: 2020-01-03

Answer

The immunogen of M00637 anti-PAI1 Rabbit Monoclonal antibody is A synthesized peptide derived from human PAI1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-01-03

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-PAI1 Rabbit Monoclonal antibody M00637. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-08-02

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-02

Question

Do you have a BSA free version of anti-PAI1 Rabbit Monoclonal antibody M00637 available?

B. Singh

Verified customer

Asked: 2019-04-22

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-PAI1 Rabbit Monoclonal antibody M00637 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-04-22

Order DetailsPrice
M00637

100μL

$370
M00637-30ul

30μL sample

$99
M00637-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
M00637
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.